Datasheet

Biological Description

Specificity	Mast Cell Tryptase Mouse mAb recognizes endogenous levels of total Mast Cell Tryptase protein.
Background	Mast cell tryptase is a highly conserved, tetrameric serine protease predominantly produced and stored in large quantities within the secretory granules of mast cells, which play a central role in allergic reactions, inflammation, and tissue defense. It exists mainly as a stable tetramer formed by four identical subunits (each ~30–36 kDa), with each monomer encompassing a classic serine protease catalytic triad (serine, histidine, aspartate), multiple cysteine-rich motifs forming disulfide bonds for structural stability, and distinct heparin-binding sites that stabilize the active enzyme and localize it to the acidic, heparin-rich granule core. The arrangement of tryptase tetramers, with active sites facing an internal cavity, protects the enzyme from endogenous inhibitors and regulates substrate specificity. Synthesized as an inactive proenzyme, tryptase is processed to its active form before being released extracellularly during mast cell degranulation, which occurs in response to allergic triggers or tissue injury. It is a pivotal mediator in both innate and adaptive immunity, cleaving extracellular matrix proteins to promote tissue remodelling. It activates protease-activated receptor 2 (PAR-2) on various cell types, thereby modulating vascular tone and neurogenic inflammation. Additionally, it stimulates cytokine secretion, such as IL-6 and TNF-α, which amplify inflammatory responses. Tryptase is critically involved in processes like bronchoconstriction, increased vascular permeability, leukocyte recruitment, smooth muscle proliferation, and angiogenesis. Elevated serum tryptase is a sensitive clinical biomarker of mast cell activation and is used in diagnosing and monitoring anaphylaxis, systemic mastocytosis, chronic urticaria, and related mast cell disorders; baseline or persistently high levels may indicate disease severity or progression.

Specificity

Mast Cell Tryptase Mouse mAb recognizes endogenous levels of total Mast Cell Tryptase protein.

Background

Mast cell tryptase is a highly conserved, tetrameric serine protease predominantly produced and stored in large quantities within the secretory granules of mast cells, which play a central role in allergic reactions, inflammation, and tissue defense. It exists mainly as a stable tetramer formed by four identical subunits (each ~30–36 kDa), with each monomer encompassing a classic serine protease catalytic triad (serine, histidine, aspartate), multiple cysteine-rich motifs forming disulfide bonds for structural stability, and distinct heparin-binding sites that stabilize the active enzyme and localize it to the acidic, heparin-rich granule core. The arrangement of tryptase tetramers, with active sites facing an internal cavity, protects the enzyme from endogenous inhibitors and regulates substrate specificity. Synthesized as an inactive proenzyme, tryptase is processed to its active form before being released extracellularly during mast cell degranulation, which occurs in response to allergic triggers or tissue injury. It is a pivotal mediator in both innate and adaptive immunity, cleaving extracellular matrix proteins to promote tissue remodelling. It activates protease-activated receptor 2 (PAR-2) on various cell types, thereby modulating vascular tone and neurogenic inflammation. Additionally, it stimulates cytokine secretion, such as IL-6 and TNF-α, which amplify inflammatory responses. Tryptase is critically involved in processes like bronchoconstriction, increased vascular permeability, leukocyte recruitment, smooth muscle proliferation, and angiogenesis. Elevated serum tryptase is a sensitive clinical biomarker of mast cell activation and is used in diagnosing and monitoring anaphylaxis, systemic mastocytosis, chronic urticaria, and related mast cell disorders; baseline or persistently high levels may indicate disease severity or progression.

Usage Information

Application

WB, IHC, IF, ELISA

Dilution

IHC

1:10,000

Reactivity

Dog, Monkey, Cat, Rat

Source

Mouse

MW

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/15200544/
https://pubmed.ncbi.nlm.nih.gov/24793463/

Mast Cell Tryptase Mouse mAb

Biological Description

Usage Information

References

Application Data