LIMP-2/SCARB2 Rabbit mAb

Catalog No.: F4239

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:50 IF1:200 - 1:800

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
54 kDa, 80 kDa

Datasheet & SDS

Biological Description

Specificity
LIMP-2/SCARB2 Rabbit mAb detects endogenous levels of total LIMP-2/SCARB2 protein.

Clone
C11F4

Synonym(s)
Lysosome membrane protein 2, 85 kDa lysosomal membrane sialoglycoprotein (LGP85), Lysosome membrane protein II (LIMP II), Scavenger receptor class B member 2, Scarb2

Background
LIMP-2 (lysosomal integral membrane protein type 2)/SCARB2, is a lysosomal membrane glycoprotein and the only endomembrane member of the CD36 scavenger receptor family, which also includes SR-B1 and CD36. Structurally, it contains two transmembrane domains, short cytosolic tails, and a large luminal ectodomain with a hydrophobic tunnel-like cavity that binds and transports cholesterol. Highly expressed in lysosomes, LIMP-2 serves multiple functions: it delivers the lysosomal enzyme β-glucocerebrosidase, acts as a receptor for certain enteroviruses, and mediates lysosomal cholesterol export in parallel with NPC1/2. By shuttling cholesterol (and potentially other lipids) from the lysosomal lumen to the limiting membrane and lipid droplets, LIMP-2 regulates cholesterol homeostasis, LDL receptor expression, and SREBP-2 signaling, linking it to lipid metabolism and lysosomal storage disorders.

Background

LIMP-2 (lysosomal integral membrane protein type 2)/SCARB2, is a lysosomal membrane glycoprotein and the only endomembrane member of the CD36 scavenger receptor family, which also includes SR-B1 and CD36. Structurally, it contains two transmembrane domains, short cytosolic tails, and a large luminal ectodomain with a hydrophobic tunnel-like cavity that binds and transports cholesterol. Highly expressed in lysosomes, LIMP-2 serves multiple functions: it delivers the lysosomal enzyme β-glucocerebrosidase, acts as a receptor for certain enteroviruses, and mediates lysosomal cholesterol export in parallel with NPC1/2. By shuttling cholesterol (and potentially other lipids) from the lysosomal lumen to the limiting membrane and lipid droplets, LIMP-2 regulates cholesterol homeostasis, LDL receptor expression, and SREBP-2 signaling, linking it to lipid metabolism and lysosomal storage disorders.

References
https://pubmed.ncbi.nlm.nih.gov/31387993/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%