LB-100

For research use only.

Catalog No.S7537

14 publications

LB-100 Chemical Structure

Molecular Weight(MW): 268.31

LB-100 is a water soluble protein phosphatase 2A (PP2A) inhibitor with IC50s of 0.85 μM and 3.87 μM in BxPc-3 and Panc-1 cells.

Size Price Stock Quantity  
USD 147 In stock
USD 477 In stock
USD 997 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's LB-100 has been cited by 14 publications

2 Customer Reviews

  • LB-100 induced HDAC2 S394 phosphorylation.

    Exp Mol Med, 2018, 50(7):83. LB-100 purchased from Selleck.

  • LB-100 (a protein phosphatase-2A inhibitor) induces a significant increase for hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF)-C expression by enhancing phosphatidylinositol 3-kinase (PI3K)p85/AKT/ mechanistic target of rapamycin (mTOR) signaling. Western blot analysis results for phosphorylated and total PI3Kp85/AKT/mTOR, HIF-1α, and VEGF-C with or without monocyte chemoattractant protein (MCP)-1 or LB-100 treatment. Bar graphs show quantitative results by densitometric analysis. n = 3 or more independent replications. ∗P < 0.05, ∗∗P < 0.01. Gapdh, glyceraldehyde-3-phosphate dehydrogenase; p, phosphorylated.

    Am J Pathol, 2017, 187(8):1736-1749. LB-100 purchased from Selleck.

Purity & Quality Control

Choose Selective phosphatase Inhibitors

Biological Activity

Description LB-100 is a water soluble protein phosphatase 2A (PP2A) inhibitor with IC50s of 0.85 μM and 3.87 μM in BxPc-3 and Panc-1 cells.
Targets
PP2A [1]
In vitro

LB-100 inhibits the cell growth with IC50 of 2.3 μM (in BxPc-3) or 1.7 μM (in Panc-1 cell). In BxPc-3, Panc-1, and SW1990 cells, LB-100 reduces the PP2A activity by 30-50%. LB-100 increases concentration of doxorubicin within cells (2.5 fold to control) and sensitizes tumor cells to the cytotoxicity of doxorubicin. LB-100 increaseds VEGF secretion, and thus enhances HIF-1α-VEGF mediated angiogenesis.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CH-157 cells MlTDSpVv[3Srb36gZZN{[Xl? NEj1SpgzNjVizszN MnTTN{Bpd3W{cx?= NIHtfHVNSi1zMECgd4lodmmoaXPhcpRtgSCnbnjhcoNm\CC2aHWgdJJweG:{dHnvckBw\iClZXzsd{BqdiCJMj;NMi=> MV[yPVE6QTByNh?=
DAOY cells M1f1[mNmdGxidnnhZoltcXS7IHHzd4F6 M4HGb2lEPTB;Mj65JO69VSxidYPpcochYFSWIHHzd4F6ew>? NHTxT28zPjd7OU[3NC=>
D341 cells M4K1cmNmdGxidnnhZoltcXS7IHHzd4F6 Mn:4TWM2OD1zLkmg{txONCC3c3nu[{BZXFRiYYPzZZl{ MVGyOlc6QTZ5MB?=
D283 cells Mof0R4VtdCC4aXHibYxqfHliYYPzZZk> MnnZTWM2OD1yLkmg{txONCC3c3nu[{BZXFRiYYPzZZl{ M{f2RlI3Pzl7Nkew
U251 cells NWn1XnpCTnWwY4Tpc44h[XO|YYm= MnHsNkDPxE1? NYHRTZl5OyCqb4Xyd{BidmRiNjDoc5Vzew>? M4XXRpRz\WG2bXXueEB4cXSqIEKg{txOKEyEMUCwJJJm\HWlZXSgVHAzSSCjY4Tpeol1gSC2bzC2NUUhd2ZiY3;ueJJwdCClZXzsd{Bi\nSncjDic5RpKHSqcnXlJIFv\CC|aYigbI92enNib3[gUGIyODBiZYjwc5N2emVw NXLDclFsOjV7M{m3OlI>
SKOV-3 M{LrVmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkjvO|IhcA>? M2XkfGlEPTBiPTCxNE4yKMLzIEGuPEDPxE1? NFzheGwzPTN5Nk[wPC=>
OVCAR-8 NX7IVmlXS3m2b4TvfIlkcXS7IHHzd4F6 MXG3NkBp MX\JR|UxKD1iNT61JOKyKDBwNTFOwG0> MnrBNlU{PzZ4MEi=
PEO-4 NGPjRodEgXSxdH;4bYNqfHliYYPzZZk> NUPDVW5bPzJiaB?= MoCzTWM2OCB;IEWgxtEhOC54IN88US=> M4fnWlI2Ozd4NkC4
PEO-6 NYXYOXpyS3m2b4TvfIlkcXS7IHHzd4F6 M1jx[|czKGh? NV3wd|VHUUN3MDC9JFUvOSEEsTCwMlIh|ryP MXmyOVM4PjZyOB?=
PEO1-Brca2 Missense MnPsR5l1d3SxeHnjbZR6KGG|c3H5 M4niXFczKGh? MoP6TWM2OCB;IE[uNkDDuSBzLkWg{txO MnHTNlU{PzZ4MEi=
PEO1-Brca2 STOP NI\WRXFEgXSxdH;4bYNqfHliYYPzZZk> M2K1XFczKGh? MWjJR|UxKD1iNj6yJOKyKDFwMDFOwG0> NVLoeFVCOjV|N{[2NFg>
Huh-7 MXPQVFJCKGGldHn2bZR6KGG|c3H5dy=> Mn[5OUDPxG2xbD;M MYGyJIg> MX7y[YR2[2WmIITo[UBi[3Srdnn0fUBw\iCSUELBJJRwKGGkb4X0JFcxLQ>? MUOyOFg3PzJ2OR?=
HepG2 NG\vNZZRWDKDIHHjeIl3cXS7IHHzd4F6ew>? MYq1JO69dW:uL1y= M1X5clIhcA>? M4DhRpJm\HWlZXSgeIhmKGGldHn2bZR6KG:oIGDQNmEhfG9iYXLveZQhPzBn MnvoNlQ5Pjd{NEm=
HL-7702 NGnhcnhRWDKDIHHjeIl3cXS7IHHzd4F6ew>? NGnWNoY2KM7:bX;sM2w> M3nPdlIhcA>? M{\jPZJm\HWlZXSgeIhmKGGldHn2bZR6KG:oIGDQNmEhfG9iYXLveZQhPzBn MlnzNlQ5Pjd{NEm=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AMPKα1 / AMPKα1 / p-ACC / ACC / p-p70S6K1 / p-4EBP1 ; 

PubMed: 29221169     


HCT-116 cells were treated with LB-100 at 10 μM, cells were further cultured for the designated time. Expressions of listed proteins were tested by Western blotting assay.  

Cyclin D1 / c-myc / mcl-1 / Hsp90 ; 

PubMed: 29199006     


LB-100 down-regulates expression of STAT3 target genes. Western blot analysis of IOMM-LEE, GAR and CH-157 cells treated with increasing LB100 concentration demonstrates dose-dependent decrease in STAT3 target genes, including mcl-1, c-myc, and cyclin D.

29221169 29199006
Growth inhibition assay
Cell division ; 

PubMed: 29844427     


In vitro proliferation of CD4+ T cells in the presence of LB-100 dose titration, measured by dilution of the cytosolic CFSE. Percentage of cells divided was plotted against concentration of LB-100.

Cell viability ; 

PubMed: 29199006     


Three meningioma cell lines IOMM-LEE, GAR and CH-157 were used in vitro. XTT assays were performed after 48 hours of treatment. Increasing concentrations of LB-100 reduced cell viability, but cytotoxic effect was seen only at high drug concentration with 䲧疝Ỵ疞㧀

29844427 29199006
In vivo In a mouse pancreatic cancer xenograft model, LB-100 (2 mg/kg, i.p.) enhances chemotherapy of doxorubicin. LB-100 causes higher density of microvessel in tumors and rapid blood flow at the surface of tumors. [1]

Protocol

Kinase Assay:

[1]

- Collapse

PP2A activity assays:

Cultured pancreatic cancer cells are treated with IC50 of LB-100 for each cell line or equal volume of vehicle for 2 hours, and PP2A activity assays are then performed using Ser/Thr phosphatase assay kit. Cells are lysed with an ultrasonic cell disruptor, and the PP2A concentration is measured using a Ser/Thr phosphatase assay kit according to the instructions. Assays for each cell line are performed in triplicate.
Cell Research:

[1]

- Collapse
  • Cell lines: BxPc-3, and Panc-1 cell lines
  • Concentrations: ~ 10 μM
  • Incubation Time: 48 h
  • Method:

    Cytotoxicity is conducted by using a Cell Counting Kit-8. Cells are seeded in 96-well plates with a density of 3000 cells per well and are assessed after treatments following the CCK-8 protocol. Relative cytotoxicity is expressed as a percentage of specific controls.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: BALB/c nude mice bearing Panc-1 xenograft
  • Dosages: 2 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 53 mg/mL (197.53 mM)
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 268.31
Formula

C13H20N2O4

CAS No. 1026680-07-8
Storage powder
in solvent
Synonyms N/A
Smiles CN1CCN(CC1)C(=O)C2C3CCC(O3)C2C(O)=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy LB-100|LB-100 ic50|LB-100 price|LB-100 cost|LB-100 solubility dmso|LB-100 purchase|LB-100 manufacturer|LB-100 research buy|LB-100 order|LB-100 mouse|LB-100 chemical structure|LB-100 mw|LB-100 molecular weight|LB-100 datasheet|LB-100 supplier|LB-100 in vitro|LB-100 cell line|LB-100 concentration|LB-100 nmr|LB-100 in vivo|LB-100 clinical trial|LB-100 inhibitor|LB-100 Others inhibitor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID