Catalog No.S7537

For research use only.

LB-100 is a water soluble protein phosphatase 2A (PP2A) inhibitor with IC50s of 0.85 μM and 3.87 μM in BxPc-3 and Panc-1 cells.

LB-100 Chemical Structure

CAS No. 1026680-07-8

Selleck's LB-100 has been cited by 32 publications

Purity & Quality Control

Choose Selective PP2A Inhibitors

Biological Activity

Description LB-100 is a water soluble protein phosphatase 2A (PP2A) inhibitor with IC50s of 0.85 μM and 3.87 μM in BxPc-3 and Panc-1 cells.
PP2A [1]
In vitro

LB-100 inhibits the cell growth with IC50 of 2.3 μM (in BxPc-3) or 1.7 μM (in Panc-1 cell). In BxPc-3, Panc-1, and SW1990 cells, LB-100 reduces the PP2A activity by 30-50%. LB-100 increases concentration of doxorubicin within cells (2.5 fold to control) and sensitizes tumor cells to the cytotoxicity of doxorubicin. LB-100 increaseds VEGF secretion, and thus enhances HIF-1α-VEGF mediated angiogenesis.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U251 cells MorBSpVv[3Srb36gZZN{[Xl? MmPnNkDPxE1? NGLaeZg{KGixdYLzJIFv\CB4IHjveZJ{ MnHheJJm[XSvZX70JJdqfGhiMjFOwG0hVEJzMECgdoVlfWOnZDDQVFJCKGGldHn2bZR6KHSxIE[xKUBw\iClb370do9tKGOnbHzzJIFnfGW{IHLveIghfGi{ZXWgZY5lKHOreDDoc5VzeyCxZjDMRlExOCCneIDvd5Vz\S5? M4GyUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUO5O|YzLz5{NUmzPVc3OjxxYU6=
D283 cells MV\D[YxtKH[rYXLpcIl1gSCjc4PhfS=> M2HWVGlEPTB;MD65JO69VSxidYPpcochYFSWIHHzd4F6ew>? M2LvRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{m5OlcxLz5{Nke5PVY4ODxxYU6=
DAOY cells MVHD[YxtKH[rYXLpcIl1gSCjc4PhfS=> M2\XT2lEPTB;Mj65JO69VSxidYPpcochYFSWIHHzd4F6ew>? M1y1[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{m5OlcxLz5{Nke5PVY4ODxxYU6=
D341 cells M4DUWmNmdGxidnnhZoltcXS7IHHzd4F6 NUjGb2k2UUN3ME2xMlkh|ryPLDD1d4lv\yC[VGSgZZN{[Xm| MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd7OU[3NEc,OjZ5OUm2O|A9N2F-
CH-157 cells NFnEUXdHfW6ldHnvckBie3OjeR?= NFnhPWMzNjVizszN M1vJ[lMhcG:3coO= NHraPVJNSi1zMECgd4lodmmoaXPhcpRtgSCnbnjhcoNm\CC2aHWgdJJweG:{dHnvckBw\iClZXzsd{BqdiCJMj;NMi=> M4joUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm5NFA3Lz5{OUG5PVAxPjxxYU6=
SKOV-3 M2G0PWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2jTV|czKGh? NUizRlZTUUN3MDC9JFExNjFiwsGgNU45KM7:TR?= NUTERWhvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO|Y3ODhpPkK1N|c3PjB6PD;hQi=>
OVCAR-8 NVTtT2RsS3m2b4TvfIlkcXS7IHHzd4F6 M2rrOFczKGh? MWPJR|UxKD1iNT61JOKyKDBwNTFOwG0> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5Nk[wPEc,OjV|N{[2NFg9N2F-
PEO-4 NGPvTmhEgXSxdH;4bYNqfHliYYPzZZk> MmTjO|IhcA>? NVu3PYRXUUN3MDC9JFUhyrFiMD62JO69VQ>? NILie5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO3OlYxQCd-MkWzO|Y3ODh:L3G+
PEO-6 NFPRe5VEgXSxdH;4bYNqfHliYYPzZZk> NFe0XXo4OiCq M1rTdWlEPTBiPTC1MlEhyrFiMD6yJO69VQ>? NUi5cpBGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO|Y3ODhpPkK1N|c3PjB6PD;hQi=>
PEO1-Brca2 Missense NV\iN5luS3m2b4TvfIlkcXS7IHHzd4F6 NHPIUI04OiCq MojqTWM2OCB;IE[uNkDDuSBzLkWg{txO Ml;vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|N{[2NFgoRjJ3M{e2OlA5RC:jPh?=
PEO1-Brca2 STOP MVfDfZRwfG:6aXPpeJkh[XO|YYm= NFLWWnI4OiCq NWK3WWF7UUN3MDC9JFYvOiEEsTCxMlAh|ryP NFe2[Yk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO3OlYxQCd-MkWzO|Y3ODh:L3G+
Huh-7 NI\0OoVRWDKDIHHjeIl3cXS7IHHzd4F6ew>? NWfzPWxpPSEQvH3vcE9N M2r1UVIhcA>? Ml\YdoVlfWOnZDD0bIUh[WO2aY\peJkhd2ZiUGCyRUB1dyCjYn;1eEA4OCV? Ml[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NkeyOFkoRjJ2OE[3NlQ6RC:jPh?=
HepG2 M1G0R3BROkFiYXP0bZZqfHliYYPzZZl{ NIjselQ2KM7:bX;sM2w> MkfyNkBp NWnLW4YyemWmdXPl[EB1cGViYXP0bZZqfHlib3[gVHAzSSC2bzDhZo92fCB5MDW= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh4N{K0PUc,OjR6NkeyOFk9N2F-
HL-7702 NIG0[JhRWDKDIHHjeIl3cXS7IHHzd4F6ew>? MnrPOUDPxG2xbD;M NWfKWYI3OiCq NYfJWWJLemWmdXPl[EB1cGViYXP0bZZqfHlib3[gVHAzSSC2bzDhZo92fCB5MDW= NGK1WHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi2O|I1QSd-MkS4OlczPDl:L3G+
Methods Test Index PMID
Western blot Cyclin D1 / c-myc / mcl-1 / Hsp90 29199006
Growth inhibition assay Cell division ; Cell viability 29844427 29199006
In vivo In a mouse pancreatic cancer xenograft model, LB-100 (2 mg/kg, i.p.) enhances chemotherapy of doxorubicin. LB-100 causes higher density of microvessel in tumors and rapid blood flow at the surface of tumors. [1]

Protocol (from reference)

Kinase Assay:


  • PP2A activity assays:

    Cultured pancreatic cancer cells are treated with IC50 of LB-100 for each cell line or equal volume of vehicle for 2 hours, and PP2A activity assays are then performed using Ser/Thr phosphatase assay kit. Cells are lysed with an ultrasonic cell disruptor, and the PP2A concentration is measured using a Ser/Thr phosphatase assay kit according to the instructions. Assays for each cell line are performed in triplicate.

Cell Research:


  • Cell lines: BxPc-3, and Panc-1 cell lines
  • Concentrations: ~ 10 μM
  • Incubation Time: 48 h
  • Method:

    Cytotoxicity is conducted by using a Cell Counting Kit-8. Cells are seeded in 96-well plates with a density of 3000 cells per well and are assessed after treatments following the CCK-8 protocol. Relative cytotoxicity is expressed as a percentage of specific controls.

Animal Research:


  • Animal Models: BALB/c nude mice bearing Panc-1 xenograft
  • Dosages: 2 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 268.31


CAS No. 1026680-07-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN1CCN(CC1)C(=O)C2C3CCC(C2C(=O)O)O3

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03886662 Unknown status Drug: LB-100 Myelodysplastic Syndromes Lixte Biotechnology Holdings Inc. April 2019 Phase 1|Phase 2
NCT03027388 Enrolling by invitation Drug: LB-100 Astrocytoma Grades II III and IV|Glioblastoma Multiforme|Giant Cell Glioblastoma|Glioma|Oligodendrogliomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 9 2019 Phase 2
NCT01837667 Completed Drug: LB-100 for Injection|Drug: Docetaxel Tumors|Neoplasms|Cancer Lixte Biotechnology Holdings Inc. February 2013 Phase 1

(data from, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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