ID-8

Catalog No.S7327

For research use only.

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

ID-8 Chemical Structure

CAS No. 147591-46-6

Purity & Quality Control

Choose Selective DYRK Inhibitors

Biological Activity

Description ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.
Targets
DYRK [1]
In vitro

ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. [1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. [2]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: Human embryonic stem cells
  • Concentrations: ~10 μM
  • Incubation Time: 7 days
  • Method: Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

Solubility (25°C)

In vitro

DMSO 60 mg/mL
(201.14 mM)
Ethanol 2 mg/mL
(6.7 mM)
Water Insoluble

Chemical Information

Molecular Weight 298.29
Formula

C16H14N2O4

CAS No. 147591-46-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C2=C(N1C3=CC=C(C=C3)OC)C=C(C=C2)O)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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