GSK650394

Catalog No.S7209

For research use only.

GSK650394 is a serum- and glucocorticoid-regulated kinase-1 inhibitor with IC50 of 62 nM and 103 nM for SGK1 and SGK2, respectively.

GSK650394 Chemical Structure

CAS No. 890842-28-1

Selleck's GSK650394 has been cited by 19 publications

Purity & Quality Control

Choose Selective SGK Inhibitors

Biological Activity

Description GSK650394 is a serum- and glucocorticoid-regulated kinase-1 inhibitor with IC50 of 62 nM and 103 nM for SGK1 and SGK2, respectively.
Targets
SGK1 [1]
(Cell-free assay)
SGK2 [1]
(Cell-free assay)
62 nM 103 nM
In vitro

GSK650394 inhibits SGK1-mediated epithelial transport with an IC50 of 0.6 μM in the SCC assay. In LNCaP cells, GSK650394 inhibits the androgen-mediated enhancement of Nedd4-2 phosphorylation and androgen-mediated cell growth. [2] GSK650394 counteracts the Cortisol-induced decrease in neurogenesis, changes in Hedgehog signaling, and GR nuclear translocation. [2] GSK 650394 inhibits replication of influenza virus by impairing the export of influenza vRNPs into the cytoplasm of A549 cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human M-1 cells MX;GeY5kfGmxbjDhd5NigQ>? NGSzOFJKdmirYnn0bY9vKG:oIGPHT|EudWWmaXH0[YQh\XCrdHjlcIlidCC|b3TpeY0h[2ijbn7lcEBi[3Srdnn0fUBqdiCqdX3hckBONTFiY3XscJMh[XO|ZYPz[YQh[XNic3jvdpQh[2m{Y4XpeEBkfXK{ZX70JIJ6KHeqb3zlJINmdGxiZXzlZ5Rzd3CqeYPpc4xw\2mlYXygeJJidnOncHn0bIVtcWGuIHX4dIVzcW2nboSsJGlEPTB;MD61PEDPxE1? NUPSV4tFOTl2OUe3OFU>
human MV-4-11 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknETY5pcWKrdHnvckBw\iCqdX3hckBOXi12LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPjR7IN88US=> MYfTRW5ITVJ?
human Daoy cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXfJcohq[mm2aX;uJI9nKGi3bXHuJGRid3liY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkWxOVMzKM7:TR?= M4rVbHNCVkeHUh?=
human BFTC-905 cell NEmyb|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLTTXRRUW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwN{i0OVUh|ryP NW\2WFY4W0GQR1XS
human KM12 cell NVnPOFhjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXvJcohq[mm2aX;uJI9nKGi3bXHuJGtOOTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki1NFY6KM7:TR?= NWjJR5d2W0GQR1XS
human GDM-1 cell MkTUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVrJcohq[mm2aX;uJI9nKGi3bXHuJGdFVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64O|k{PyEQvF2= MnP4V2FPT0WU
human RT-112 cell NEGxfmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mme1TY5pcWKrdHnvckBw\iCqdX3hckBTXC1zMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlAzPzh{IN88US=> MYrTRW5ITVJ?
human MONO-MAC-6 cell M{HucGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXjJcohq[mm2aX;uJI9nKGi3bXHuJG1QVk9vTVHDMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF6MEm3JO69VQ>? MUTTRW5ITVJ?
human HUTU-80 cell NHLtb|dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlvDTY5pcWKrdHnvckBw\iCqdX3hckBJXVSXLUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODF5MjFOwG0> NF7ld5VUSU6JRWK=
human HGC-27 cell NYXrNIU3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFXzVWtKdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzB6MkGg{txO MWTTRW5ITVJ?
human LCLC-97TM1 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mkm4TY5pcWKrdHnvckBw\iCqdX3hckBNS0yFLUm3WG0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5|Nke2PEDPxE1? M2\M[XNCVkeHUh?=
human LXF-289 cell NIn1OnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF;nXmVKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjRyMUWyJO69VQ>? NW\ERXVIW0GQR1XS
human HLE cell NEPhN25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWPJcohq[mm2aX;uJI9nKGi3bXHuJGhNTSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwNEK2PFMh|ryP NX2xZldDW0GQR1XS
human RKO cell NHTibHpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWf0[lAxUW6qaXLpeIlwdiCxZjDoeY1idiCUS1:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlU4QDZ7IN88US=> NVLCOlBwW0GQR1XS
human SNG-M cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1vnTWlvcGmkaYTpc44hd2ZiaIXtZY4hW06JLV2gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlc6QDV|IN88US=> NXTDVGhVW0GQR1XS
human MCF7 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWfD[mpkUW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45OTd6IN88US=> NUH0T5luW0GQR1XS
human A431 cell NHfzbVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFvNOWJKdmirYnn0bY9vKG:oIHj1cYFvKEF2M{GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlg2QTB{IN88US=> MoPZV2FPT0WU
human BC-3 cell NFfRR|RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHvCbIxKdmirYnn0bY9vKG:oIHj1cYFvKEKFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlg4ODl2IN88US=> NGXsOGtUSU6JRWK=
human A427 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4Lid2lvcGmkaYTpc44hd2ZiaIXtZY4hSTR{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPFc3OTFizszN M{TtO3NCVkeHUh?=
human SW620 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHfkRZJKdmirYnn0bY9vKG:oIHj1cYFvKFOZNkKwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU46OjhzNjFOwG0> M17KTHNCVkeHUh?=
human HOS cell NUm0blVHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NE\QOndKdmirYnn0bY9vKG:oIHj1cYFvKEiRUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPVc{OTJizszN M3K1W3NCVkeHUh?=
human MFH-ino cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnHETY5pcWKrdHnvckBw\iCqdX3hckBOTkhvaX7vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU46QDZ3ODFOwG0> MkLoV2FPT0WU
human TE-12 cell NHPNVYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWDJbmozUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQTlzMkig{txO NUC2NVkzW0GQR1XS
human KU812 cell NWPrbG5uT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{LaWWlvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlE5PzFizszN NIXYOXdUSU6JRWK=
human NCI-H2342 cell MoDwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlnRTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKzOFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjF7MEWzJO69VQ>? NYDaboF4W0GQR1XS
human BC-1 cell NFrJ[VBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4jXcmlvcGmkaYTpc44hd2ZiaIXtZY4hSkNvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuOVE3PTFizszN MmnwV2FPT0WU
human TE-15 cell NXHSdJA5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M37xXmlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlU6OThzIN88US=> MoraV2FPT0WU
human T84 cell NHTzPZNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NE\BS2NKdmirYnn0bY9vKG:oIHj1cYFvKFR6NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuOlA1PDZizszN NX3sSINFW0GQR1XS
human SW1710 cell NXTEPHNtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFTHdJlKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvPzByOEKg{txO M3v0enNCVkeHUh?=
human CAL-12T cell M1S5[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NECzc2FKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2xNnQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjdzMUW4JO69VQ>? NVexempCW0GQR1XS
human NKM-1 cell NGnD[YpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX;qdFMxUW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvPzZ5M{Gg{txO NHr5ToZUSU6JRWK=
human OCUB-M cell NHL5O45Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3\LbGlvcGmkaYTpc44hd2ZiaIXtZY4hV0OXQj3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk44QDh4MTFOwG0> M3PJXnNCVkeHUh?=
human 786-0 cell NIOyNnpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWjJcohq[mm2aX;uJI9nKGi3bXHuJFc5Pi1yIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj65OFgzPSEQvF2= M{Cwc3NCVkeHUh?=
human EW-13 cell NV3ldIRnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGVYNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6wNVU2QCEQvF2= NIT1TpBUSU6JRWK=
human 5637 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnzhTY5pcWKrdHnvckBw\iCqdX3hckA2PjN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yNFY3QSEQvF2= NFLhTIpUSU6JRWK=
human PA-1 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4LuO2lvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuN|MxQDNizszN NFfjfWJUSU6JRWK=
human NCI-H2122 cell NXLOWGkyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPlOWtPUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIyOjJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkOzN{DPxE1? NGjr[JNUSU6JRWK=
human NCI-H460 cell NWDPTZdKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVvJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNE[wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41ODRzIN88US=> M4i1XXNCVkeHUh?=
human CHL-1 cell MmP4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4HyNWlvcGmkaYTpc44hd2ZiaIXtZY4hS0iOLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlQ6PjZ3IN88US=> NYLYVXB2W0GQR1XS
human MOLT-16 cell NHnGOWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIr6S2NKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjVzNUW1JO69VQ>? MYXTRW5ITVJ?
human HCT-15 cell M1LRWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYrJcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOlI5PDJizszN MWXTRW5ITVJ?
human G-361 cell NU\nS3hKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2\QfmlvcGmkaYTpc44hd2ZiaIXtZY4hTy1|NkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlcyPzR3IN88US=> NVHyWIRrW0GQR1XS
human SK-LU-1 cell MoW4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWLJcohq[mm2aX;uJI9nKGi3bXHuJHNMNUyXLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlk4ODF2IN88US=> MoLtV2FPT0WU
human ES4 cell NGf3T|ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIXJdVVKdmirYnn0bY9vKG:oIHj1cYFvKEWVNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNFYxPjlizszN NVvmbYE6W0GQR1XS
human TE-8 cell NWe1bmU4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnPKTY5pcWKrdHnvckBw\iCqdX3hckBVTS16IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xNVU5OiEQvF2= MVXTRW5ITVJ?
human IGR-1 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnzMTY5pcWKrdHnvckBw\iCqdX3hckBKT1JvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNlM{KM7:TR?= Mn3sV2FPT0WU
human SW954 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF7N[o1KdmirYnn0bY9vKG:oIHj1cYFvKFOZOUW0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE41PjF{NDFOwG0> NIO4bnFUSU6JRWK=
human A375 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4\XNmlvcGmkaYTpc44hd2ZiaIXtZY4hSTN5NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNlU1QTJizszN NEXUW4NUSU6JRWK=
human CAL-27 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlfvTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM{OjJzIN88US=> MUTTRW5ITVJ?
human CAL-51 cell NHPqR5RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYPJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|U2QDNizszN MoLSV2FPT0WU
human SK-LMS-1 cell M1y3cmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYnzW|lpUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwM{iyOVUh|ryP M3nHdHNCVkeHUh?=
human HT-1080 cell NUm1Tmh4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmLJTY5pcWKrdHnvckBw\iCqdX3hckBJXC1zMEiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU41QTN2NzFOwG0> Ml2zV2FPT0WU
human NCI-H1299 cell NFrpPJVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml3TTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEGyPVkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl6NUW3JO69VQ>? NUPwd4loW0GQR1XS
human HT-29 cell M{fYTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3Hwc2lvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlI3QDR|IN88US=> MV3TRW5ITVJ?
human A388 cell MmXjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVfEVXdTUW6qaXLpeIlwdiCxZjDoeY1idiCDM{i4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{42QTl4NTFOwG0> NYfibJF{W0GQR1XS
human SK-MEL-30 cell NYC1dZpCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUGxZoFFUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOzBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15Lkm3NlE2KM7:TR?= MXjTRW5ITVJ?
human CMK cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKGi3bXHuJGNOUyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwMkWzO|ch|ryP MoT5V2FPT0WU
human HuP-T4 cell NVvKPJdST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmrtTY5pcWKrdHnvckBw\iCqdX3hckBJfVBvVESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlUxOjN2IN88US=> MmW4V2FPT0WU
human SF126 cell NUe3cJFxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJHNHOTJ4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD64NFIxQCEQvF2= NEm0d2FUSU6JRWK=
human MKN1 cell NIHidWpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHTIZolKdmirYnn0bY9vKG:oIHj1cYFvKE2NTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlE5PTZ3IN88US=> NIf2RXBUSU6JRWK=
human LoVo cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmXZTY5pcWKrdHnvckBw\iCqdX3hckBNd1[xIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT65NVI6OiEQvF2= M161PXNCVkeHUh?=
human HEL cell NWXSfYg{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M332UGlvcGmkaYTpc44hd2ZiaIXtZY4hUEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOlgh|ryP NYL1PXB3W0GQR1XS
human SW872 cell NXHzdZcxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmLyTY5pcWKrdHnvckBw\iCqdX3hckBUXzh5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlY5QTZizszN M{n6SnNCVkeHUh?=
human SW982 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mnr4TY5pcWKrdHnvckBw\iCqdX3hckBUXzl6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlc6OTZizszN M3j5UHNCVkeHUh?=
human HuH-7 cell MofLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{DDbWlvcGmkaYTpc44hd2ZiaIXtZY4hUHWKLUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE4zPzh|IN88US=> M3f3O3NCVkeHUh?=
human DU-145 cell MojHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHLxe4NKdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjR7NUWg{txO MmS5V2FPT0WU
human MC116 cell NWn2PJJVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrNb|hTUW6qaXLpeIlwdiCxZjDoeY1idiCPQ{GxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjJ4N{[g{txO NYDrS2pDW0GQR1XS
human H4 cell MmLqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE[wWXBKdmirYnn0bY9vKG:oIHj1cYFvKEh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkGuOFI1PCEQvF2= M1fXfHNCVkeHUh?=
human SK-MEL-3 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn\3TY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjB{Mkig{txO NFjERVdUSU6JRWK=
Assay
Methods Test Index PMID
Western blot p21 / LC3 / p62 / PARP / Cleaved PARP ; c-Jun / NRF2 ; p-SGK1 / SGK1 ; p-SGK2 / SGK2 29017179 31136958 30873046 26392083
Immunofluorescence LC3 29017179
Growth inhibition assay Cell viability 29017179

Protocol (from reference)

Kinase Assay:[1]
  • Scintillation proximity assay (SPA):

    SGK1 S422D (60–431 aa; 0.275 μg/mL final concentration) or SGK2 (0.875 μg/mL final concentration) are activated by PDK1 (1.1 μg/mL final concentration) in a buffer consisting of 50 mM Tris (pH 7.5), 0.1 mM EGTA, 0.1 mM EDTA, 10 mM MgCl2, 0.1% β-mercaptoethanol, 1 mg/mL BSA, and ATP (final concentration of 0.15 mM) and incubated for 30 min at 30°C. SGK2 is prepared exactly as described for SGK1, except it corresponded to the full-length protein. A solution containing biotinylated CROSStide peptide at a final concentration of 75 μM and γ32P-ATP corresponding to 2×106 cpm is prepared in the reaction buffer. In a 96-well plate, 5 μL of GSK650394 is added to 25 μL of the activated enzyme mixture. To this, 20 μL of the CROSStide mixture is added and incubated for 1 h at room temperature. Next, 50 μL of a 25 mg/mL slurry of streptavidin-coated SPA beads in PBS with 0.1 M EDTA, pH 8.0 is added. The plate is then sealed and centrifuged for 8 min at 2000 rpm, and the signal is detected by measuring for 30 sec/well in a Packard TopCount NXT Scintillation Counter. The IC50 values of the inhibition of SGK1 and SGK2 activities by GSK650394 are calculated from these data using GraphPad Prism 3 Software.

Cell Research:[1]
  • Cell lines: LNCaP cells
  • Concentrations: ~10 μM
  • Incubation Time: 7 days
  • Method: LNCaP cells are plated at a density of 5,000 cells per well in 96-well plates in 100 μL PRF-RPMI 1640, supplemented with 8% CS-FBS, 0.1 mM NEAA, and 1 mM NaPyr. At day three, cells are treated with hormone with or without GSK650394 by removing 50 μL of the media and replacing this with 50 μL of PRF-RPMI 1640 with 8% CS-FBS, NEAA, NaPyr containing a 2X concentration of the appropriate hormone/inhibitor treatment. At days 5 and 7, the treatment is repeated. On the tenth day, the media is removed and the relative cell number is measured using the FluoReporter Blue assay according to the manufacturer’s instructions.

Solubility (25°C)

In vitro

DMSO 76 mg/mL
(198.71 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 382.45
Formula

C25H22N2O2

CAS No. 890842-28-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CCC(C1)C2=C(C=CC(=C2)C3=CNC4=C3C=C(C=N4)C5=CC=CC=C5)C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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