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GSK650394

For research use only.

Catalog No.S7209

18 publications

GSK650394 Chemical Structure

CAS No. 890842-28-1

GSK650394 is a serum- and glucocorticoid-regulated kinase-1 inhibitor with IC50 of 62 nM and 103 nM for SGK1 and SGK2, respectively.

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Selleck's GSK650394 has been cited by 18 publications

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  • BV-2 cells were incubated in the absence or presence of gsk650394 (1 μM) for 30 min, followed by LPS (500 ng/ml) administration for 30 min, and then fixed. NF-κB p65 protein was visualized by indirect immunofluorescence staining using an antibody for NF-κB p65. For nuclear staining, the cells were stained with DAPI. Bar = 20 μm.

    Biochem Biophys Res Commun, 2016, 478(1):53-59. GSK650394 purchased from Selleck.

  • GSK650394 treatment reduces steady state levels of BoHV-1 proteins. Cells were grown prior to infection. Three hours prior to infection 25 nM of GSK650394 was added to the designated cultures. CRIB cells were infected withBoHV-1 (moi=2) and then stripped FBS added. At the designated times after infection (hours), whole cell lysate (50 ug protein) was prepared and Western Blots performedusing the designated antibodies directed against a viral protein. Arrows denote the position of bICP0 (97 kD), bICP4 (178 kD), bICP22 (45 kD), VP16 (65 kD), and the bracketdenotes various gE forms. Tubulin was used as a loading control. The lane marked M are molecular weight markers: numbers to left are the approximate size of the respectivebands. These results are representative of 4 independent experiments. The relative levels of the respective BoHV-1 proteins were estimated by comparing the intensity ofthe bands to tubulin using a BioRad ChemiDoc MP and PMI System. The value of the respective viral protein that was first detected after infection was set at 10 and the othervalues normalized to this value. ND refers to those lanes in which the viral protein was not detected. These results are representative of three independent experiments.

    Virus Res, 2016, 222:106-112.. GSK650394 purchased from Selleck.

Purity & Quality Control

Choose Selective SGK Inhibitors

Biological Activity

Description GSK650394 is a serum- and glucocorticoid-regulated kinase-1 inhibitor with IC50 of 62 nM and 103 nM for SGK1 and SGK2, respectively.
Targets
SGK1 [1]
(Cell-free assay)
SGK2 [1]
(Cell-free assay)
62 nM 103 nM
In vitro

GSK650394 inhibits SGK1-mediated epithelial transport with an IC50 of 0.6 μM in the SCC assay. In LNCaP cells, GSK650394 inhibits the androgen-mediated enhancement of Nedd4-2 phosphorylation and androgen-mediated cell growth. [2] GSK650394 counteracts the Cortisol-induced decrease in neurogenesis, changes in Hedgehog signaling, and GR nuclear translocation. [2] GSK 650394 inhibits replication of influenza virus by impairing the export of influenza vRNPs into the cytoplasm of A549 cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human M-1 cells Mm\hSpVv[3Srb36gZZN{[Xl? M3f2VGlvcGmkaYTpc44hd2ZiU1fLNU1u\WSrYYTl[EBmeGm2aHXsbYFtKHOxZHn1cUBkcGGwbnXsJIFkfGm4aYT5JIlvKGi3bXHuJG0uOSClZXzsd{Bie3Onc4Pl[EBieyC|aH;yeEBkcXKldXn0JIN2enKnboSgZpkhf2ixbHWgZ4VtdCCnbHXjeJJweGi7c3nvcI9ocWOjbDD0doFve2WyaYTo[Yxq[WxiZYjw[ZJqdWWwdDygTWM2OD1yLkW4JO69VQ>? NVjtTVdFOTl2OUe3OFU>
human MV-4-11 cell MoTRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUfJcohq[mm2aX;uJI9nKGi3bXHuJG1XNTRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE3PDlizszN M4r2bnNCVkeHUh?=
human Daoy cell NWTYTlVCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkHkTY5pcWKrdHnvckBw\iCqdX3hckBF[W:7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD61NVU{OiEQvF2= MX;TRW5ITVJ?
human BFTC-905 cell M2HRRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYnHPYhwUW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwN{i0OVUh|ryP MYnTRW5ITVJ?
human KM12 cell NIn0eJdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWrJcohq[mm2aX;uJI9nKGi3bXHuJGtOOTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki1NFY6KM7:TR?= MmPUV2FPT0WU
human GDM-1 cell NUHDUJF3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGdFVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64O|k{PyEQvF2= M17BR3NCVkeHUh?=
human RT-112 cell NUW1NXA3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH\UdolKdmirYnn0bY9vKG:oIHj1cYFvKFKWLUGxNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODJ5OEKg{txO MoDJV2FPT0WU
human MONO-MAC-6 cell M{PqNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWXJcohq[mm2aX;uJI9nKGi3bXHuJG1QVk9vTVHDMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF6MEm3JO69VQ>? NHnEPFVUSU6JRWK=
human HUTU-80 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlXxTY5pcWKrdHnvckBw\iCqdX3hckBJXVSXLUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODF5MjFOwG0> NXnZVVNKW0GQR1XS
human HGC-27 cell NF;sN4NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUjKW4xjUW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjNyOEKxJO69VQ>? M37JT3NCVkeHUh?=
human LCLC-97TM1 cell MkS0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2LsWmlvcGmkaYTpc44hd2ZiaIXtZY4hVEOOQz25O3ROOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwM{[3Olgh|ryP MnHhV2FPT0WU
human LXF-289 cell NVPo[lltT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4PSNWlvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDBzNUKg{txO NUfROmVqW0GQR1XS
human HLE cell MnfRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkTUTY5pcWKrdHnvckBw\iCqdX3hckBJVEViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkSyOlg{KM7:TR?= M{jMZnNCVkeHUh?=
human RKO cell M{fJUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWTkOFlzUW6qaXLpeIlwdiCxZjDoeY1idiCUS1:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlU4QDZ7IN88US=> MWnTRW5ITVJ?
human SNG-M cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXzJcohq[mm2aX;uJI9nKGi3bXHuJHNPTy2PIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63PVg2OyEQvF2= NHfJZmNUSU6JRWK=
human MCF7 cell MnPBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnrtTY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT64NVc5KM7:TR?= NGDmUnNUSU6JRWK=
human A431 cell NI\Fe4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYTCSYdNUW6qaXLpeIlwdiCxZjDoeY1idiCDNEOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45PTlyMjFOwG0> MYrTRW5ITVJ?
human BC-3 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlvRTY5pcWKrdHnvckBw\iCqdX3hckBDSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT64O|A6PCEQvF2= MVfTRW5ITVJ?
human A427 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnfDTY5pcWKrdHnvckBw\iCqdX3hckBCPDJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT64O|YyOSEQvF2= M{H2PXNCVkeHUh?=
human SW620 cell NF\U[5lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnHuTY5pcWKrdHnvckBw\iCqdX3hckBUXzZ{MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPVI5OTZizszN Mo[2V2FPT0WU
human HOS cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHTvOmZKdmirYnn0bY9vKG:oIHj1cYFvKEiRUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPVc{OTJizszN NHewdZlUSU6JRWK=
human MFH-ino cell NWTwOXd7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn;jTY5pcWKrdHnvckBw\iCqdX3hckBOTkhvaX7vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU46QDZ3ODFOwG0> NGLxZnNUSU6JRWK=
human TE-12 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoT3TY5pcWKrdHnvckBw\iCqdX3hckBVTS1zMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPVkyOjhizszN MnW3V2FPT0WU
human KU812 cell NXf6Wo5XT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4rtbWlvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlE5PzFizszN Ml;YV2FPT0WU
human NCI-H2342 cell NIXRWIxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnHyTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKzOFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjF7MEWzJO69VQ>? MorFV2FPT0WU
human BC-1 cell M2TGPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NES1NYxKdmirYnn0bY9vKG:oIHj1cYFvKEKFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlUyPjVzIN88US=> MnLwV2FPT0WU
human TE-15 cell MkXzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{jTN2lvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlU6OThzIN88US=> MXrTRW5ITVJ?
human T84 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1zXT2lvcGmkaYTpc44hd2ZiaIXtZY4hXDh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj62NFQ1PiEQvF2= M2rre3NCVkeHUh?=
human SW1710 cell NHmwR4hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1S2c2lvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk44ODB6MjFOwG0> NVPhOmYzW0GQR1XS
human CAL-12T cell MmPUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYTXcHlEUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNVJVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi55MUG1PEDPxE1? NHTodFVUSU6JRWK=
human NKM-1 cell Ml\aS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnznTY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuO|Y4OzFizszN M1P5cHNCVkeHUh?=
human OCUB-M cell MnfHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1[wb2lvcGmkaYTpc44hd2ZiaIXtZY4hV0OXQj3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk44QDh4MTFOwG0> M{LJUHNCVkeHUh?=
human 786-0 cell NFr3bYVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVq2bIdiUW6qaXLpeIlwdiCxZjDoeY1idiB5OE[tNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTR6MkWg{txO M2m2N3NCVkeHUh?=
human EW-13 cell NITnT3ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2LJ[WlvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlAyPTV6IN88US=> NVLLb5A4W0GQR1XS
human 5637 cell MlvaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MojPTY5pcWKrdHnvckBw\iCqdX3hckA2PjN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yNFY3QSEQvF2= M4jRUHNCVkeHUh?=
human PA-1 cell M2TIcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmGyTY5pcWKrdHnvckBw\iCqdX3hckBRSS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6zN|A5OyEQvF2= NGftWXNUSU6JRWK=
human NCI-H2122 cell M{PVU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHjDRpZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlEzOiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwM{OzJO69VQ>? MX7TRW5ITVJ?
human NCI-H460 cell Mnr6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEXET|dKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFYxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy52MESxJO69VQ>? MVrTRW5ITVJ?
human CHL-1 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHHpU4hKdmirYnn0bY9vKG:oIHj1cYFvKEOKTD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41QTZ4NTFOwG0> MYLTRW5ITVJ?
human MOLT-16 cell NGn4N4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmraTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{42OTV3NTFOwG0> Mn\GV2FPT0WU
human HCT-15 cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOlI5PDJizszN NI\tWWZUSU6JRWK=
human G-361 cell NGjaWJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mmq3TY5pcWKrdHnvckBw\iCqdX3hckBINTN4MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuO|E4PDVizszN M4fLNnNCVkeHUh?=
human SK-LU-1 cell MlnRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHrtV4hKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzVMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Njl5MEG0JO69VQ>? MXTTRW5ITVJ?
human ES4 cell Ml64S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFfhcHNKdmirYnn0bY9vKG:oIHj1cYFvKEWVNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNFYxPjlizszN Mn76V2FPT0WU
human TE-8 cell Mle2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MljVTY5pcWKrdHnvckBw\iCqdX3hckBVTS16IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xNVU5OiEQvF2= NFPzPIJUSU6JRWK=
human IGR-1 cell MkTVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NELwTHJKdmirYnn0bY9vKG:oIHj1cYFvKEmJUj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOzNizszN NVntRmpQW0GQR1XS
human SW954 cell M2jNeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF25W|BKdmirYnn0bY9vKG:oIHj1cYFvKFOZOUW0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE41PjF{NDFOwG0> M2LxR3NCVkeHUh?=
human A375 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGTLdVlKdmirYnn0bY9vKG:oIHj1cYFvKEF|N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlI2PDl{IN88US=> NEHoSZFUSU6JRWK=
human CAL-27 cell NUL6RndZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIDDbHBKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOzN{MkGg{txO NFTNfIVUSU6JRWK=
human CAL-51 cell M1vOfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkLwTY5pcWKrdHnvckBw\iCqdX3hckBESUxvNUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM2PTh|IN88US=> NYHGNItmW0GQR1XS
human SK-LMS-1 cell MnH4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYe0PWgxUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwM{iyOVUh|ryP MoLrV2FPT0WU
human HT-1080 cell NVSxRo1yT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYf5VY1KUW6qaXLpeIlwdiCxZjDoeY1idiCKVD2xNFgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS52OUO0O{DPxE1? MlfzV2FPT0WU
human NCI-H1299 cell MnGxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH\mbGZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVI6QSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUi1OVch|ryP M1PPZnNCVkeHUh?=
human HT-29 cell NHmyTlZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NED4TmVKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUK5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4zPjh2MzFOwG0> NY\w[4lPW0GQR1XS
human A388 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX3PVpNmUW6qaXLpeIlwdiCxZjDoeY1idiCDM{i4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{42QTl4NTFOwG0> MYPTRW5ITVJ?
human SK-MEL-30 cell NFXScY9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoTFTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytN|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04Njl5MkG1JO69VQ>? NEDRTVNUSU6JRWK=
human CMK cell M1LNWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGjnfVRKdmirYnn0bY9vKG:oIHj1cYFvKEOPSzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlU{PzdizszN MonBV2FPT0WU
human HuP-T4 cell M3ztU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn:xTY5pcWKrdHnvckBw\iCqdX3hckBJfVBvVESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlUxOjN2IN88US=> MV3TRW5ITVJ?
human SF126 cell M2C3W2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXHq[YtRUW6qaXLpeIlwdiCxZjDoeY1idiCVRkGyOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvQDB{MEig{txO NEfWWZhUSU6JRWK=
human MKN1 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYjJcohq[mm2aX;uJI9nKGi3bXHuJG1MVjFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkG4OVY2KM7:TR?= MmnWV2FPT0WU
human LoVo cell M{LsfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVHJcohq[mm2aX;uJI9nKGi3bXHuJGxwXm9iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkmxNlkzKM7:TR?= NFLtbohUSU6JRWK=
human HEL cell M1rGZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUjJcohq[mm2aX;uJI9nKGi3bXHuJGhGVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyLk[4JO69VQ>? M2\LNnNCVkeHUh?=
human SW872 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4XnSGlvcGmkaYTpc44hd2ZiaIXtZY4hW1d6N{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{43QDl4IN88US=> NYrFXo43W0GQR1XS
human SW982 cell MoTPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NETLb29KdmirYnn0bY9vKG:oIHj1cYFvKFOZOUiyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPzlzNjFOwG0> M2\GVXNCVkeHUh?=
human HuH-7 cell NHns[2xIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXXJcohq[mm2aX;uJI9nKGi3bXHuJGh2UC15IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUiuNlc5OyEQvF2= MX\TRW5ITVJ?
human DU-145 cell M{TUd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;2SlNKdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjR7NUWg{txO NVvjR2V2W0GQR1XS
human MC116 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGTlbYJKdmirYnn0bY9vKG:oIHj1cYFvKE2FMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOjZ5NjFOwG0> MmTTV2FPT0WU
human H4 cell NEfSS3dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjFwNEK0OEDPxE1? NXT2RZllW0GQR1XS
human SK-MEL-3 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFvTNmZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzBwMEKyPEDPxE1? NUnkRmxXW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p21 / LC3 / p62 / PARP / Cleaved PARP ; 

PubMed: 29017179     


PC3 cells were treated as in b; LNCaP cells were treated with DMSO or 80 μm GSK650394 (GSK) for various time points (6, 24 and 48 h). Total protein lysates were collected for western blot against p21, LC3B, p62, PARP and cleaved (CL) PARP; GAPDH was used as a loading control.

c-Jun / NRF2 ; 

PubMed: 31136958     


Western blot analysis of c-JUN and NRF2 proteins in cervical cancer cells treated with or without GSK650394. Vinculin was used as a loading control. 

p-SGK1 / SGK1 ; 

PubMed: 30873046     


Podocytes were exposed to 1, 2.5, 5, 10, or 20 μM GSK650394 for 24 h and then analyzed by immunoblot.

p-SGK2 / SGK2 ; 

PubMed: 26392083     


Western blot analysis of immunoprecipitated p-SGK2 T193 and FLAG-SGK2 from whole cell lysates in pcDNA/FLAG/SGK2-transfected ShP51 cells treated with GSK650394 (10 μM) for 30 and 60 min.

29017179 31136958 30873046 26392083
Immunofluorescence
LC3; 

PubMed: 29017179     


Immunofluorescence staining for LC3 puncta.

29017179
Growth inhibition assay
Cell viability; 

PubMed: 29017179     


Cell viability analysis using a CCK8 assay of prostate cancer cell lines after treatment with various concentrations of GSK650394 or vehicle alone for the indicated time periods. Data are the means of triplicate experiments. The results are expressed as the percentage of viable control cells treated with DMSO alone (vehicle). 

29017179

Protocol

Kinase Assay:[1]
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Scintillation proximity assay (SPA):

SGK1 S422D (60–431 aa; 0.275 μg/mL final concentration) or SGK2 (0.875 μg/mL final concentration) are activated by PDK1 (1.1 μg/mL final concentration) in a buffer consisting of 50 mM Tris (pH 7.5), 0.1 mM EGTA, 0.1 mM EDTA, 10 mM MgCl2, 0.1% β-mercaptoethanol, 1 mg/mL BSA, and ATP (final concentration of 0.15 mM) and incubated for 30 min at 30°C. SGK2 is prepared exactly as described for SGK1, except it corresponded to the full-length protein. A solution containing biotinylated CROSStide peptide at a final concentration of 75 μM and γ32P-ATP corresponding to 2×106 cpm is prepared in the reaction buffer. In a 96-well plate, 5 μL of GSK650394 is added to 25 μL of the activated enzyme mixture. To this, 20 μL of the CROSStide mixture is added and incubated for 1 h at room temperature. Next, 50 μL of a 25 mg/mL slurry of streptavidin-coated SPA beads in PBS with 0.1 M EDTA, pH 8.0 is added. The plate is then sealed and centrifuged for 8 min at 2000 rpm, and the signal is detected by measuring for 30 sec/well in a Packard TopCount NXT Scintillation Counter. The IC50 values of the inhibition of SGK1 and SGK2 activities by GSK650394 are calculated from these data using GraphPad Prism 3 Software.
Cell Research:[1]
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  • Cell lines: LNCaP cells
  • Concentrations: ~10 μM
  • Incubation Time: 7 days
  • Method: LNCaP cells are plated at a density of 5,000 cells per well in 96-well plates in 100 μL PRF-RPMI 1640, supplemented with 8% CS-FBS, 0.1 mM NEAA, and 1 mM NaPyr. At day three, cells are treated with hormone with or without GSK650394 by removing 50 μL of the media and replacing this with 50 μL of PRF-RPMI 1640 with 8% CS-FBS, NEAA, NaPyr containing a 2X concentration of the appropriate hormone/inhibitor treatment. At days 5 and 7, the treatment is repeated. On the tenth day, the media is removed and the relative cell number is measured using the FluoReporter Blue assay according to the manufacturer’s instructions.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 76 mg/mL (198.71 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 382.45
Formula

C25H22N2O2

CAS No. 890842-28-1
Storage powder
in solvent
Synonyms N/A
Smiles C1CCC(C1)C2=C(C=CC(=C2)C3=CNC4=C3C=C(C=N4)C5=CC=CC=C5)C(=O)O

In vivo Formulation Calculator (Clear solution)

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID