GSK650394

For research use only.

Catalog No.S7209

19 publications

GSK650394 Chemical Structure

CAS No. 890842-28-1

GSK650394 is a serum- and glucocorticoid-regulated kinase-1 inhibitor with IC50 of 62 nM and 103 nM for SGK1 and SGK2, respectively.

Selleck's GSK650394 has been cited by 19 publications

Purity & Quality Control

Choose Selective SGK Inhibitors

Biological Activity

Description GSK650394 is a serum- and glucocorticoid-regulated kinase-1 inhibitor with IC50 of 62 nM and 103 nM for SGK1 and SGK2, respectively.
Targets
SGK1 [1]
(Cell-free assay)
SGK2 [1]
(Cell-free assay)
62 nM 103 nM
In vitro

GSK650394 inhibits SGK1-mediated epithelial transport with an IC50 of 0.6 μM in the SCC assay. In LNCaP cells, GSK650394 inhibits the androgen-mediated enhancement of Nedd4-2 phosphorylation and androgen-mediated cell growth. [2] GSK650394 counteracts the Cortisol-induced decrease in neurogenesis, changes in Hedgehog signaling, and GR nuclear translocation. [2] GSK 650394 inhibits replication of influenza virus by impairing the export of influenza vRNPs into the cytoplasm of A549 cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human M-1 cells MnzISpVv[3Srb36gZZN{[Xl? NVq0SVl7UW6qaXLpeIlwdiCxZjDTS2syNW2nZHnheIVlKGWyaYTo[Yxq[Wxic3;kbZVuKGOqYX7u[Ywh[WO2aY\peJkhcW5iaIXtZY4hVS1zIHPlcIx{KGG|c3Xzd4VlKGG|IIPoc5J1KGOrcnP1bZQh[3W{cnXueEBjgSC5aH;s[UBk\WyuIHXs[YN1em:yaInzbY9td2erY3HsJJRz[W6|ZYDpeIhmdGmjbDDlfJBmemmvZX70MEBKSzVyPUCuOVgh|ryP MYixPVQ6Pzd2NR?=
human MV-4-11 cell NFS3cHNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2H1fWlvcGmkaYTpc44hd2ZiaIXtZY4hVVZvND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTZ2OTFOwG0> M1i2dnNCVkeHUh?=
human Daoy cell NX[1NVNsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYfIS2tGUW6qaXLpeIlwdiCxZjDoeY1idiCGYX;5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42OTV|MjFOwG0> NGHuWZVUSU6JRWK=
human BFTC-905 cell NYjOU|JiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWPJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44QDR3NTFOwG0> MVnTRW5ITVJ?
human KM12 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGtOOTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki1NFY6KM7:TR?= NW\O[5pVW0GQR1XS
human GDM-1 cell MlTwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI[yRnNKdmirYnn0bY9vKG:oIHj1cYFvKEeGTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45Pzl|NzFOwG0> MlfwV2FPT0WU
human RT-112 cell NVmxPIZGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYnJcohq[mm2aX;uJI9nKGi3bXHuJHJVNTFzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNFI4QDJizszN NInrUGZUSU6JRWK=
human MONO-MAC-6 cell M3fIS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{W3cWlvcGmkaYTpc44hd2ZiaIXtZY4hVU:QTz3NRWMuPiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUiwPVch|ryP NE\WRXNUSU6JRWK=
human HUTU-80 cell NHrqT2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3;mXmlvcGmkaYTpc44hd2ZiaIXtZY4hUFWWVT24NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzBzN{Kg{txO M3nLSnNCVkeHUh?=
human HGC-27 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGhISy1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|A5OjFizszN MoDHV2FPT0WU
human LCLC-97TM1 cell NGPuRnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYDJcohq[mm2aX;uJI9nKGi3bXHuJGxEVENvOUfUUVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjN4N{[4JO69VQ>? MnPqV2FPT0WU
human LXF-289 cell MnjuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{L6XmlvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDBzNUKg{txO MlSwV2FPT0WU
human HLE cell NFPTfYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVPJcohq[mm2aX;uJI9nKGi3bXHuJGhNTSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwNEK2PFMh|ryP MkSzV2FPT0WU
human RKO cell M4rwemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2OzUGlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT61O|g3QSEQvF2= NXXGPYtuW0GQR1XS
human SNG-M cell NGXlW49Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVSxWHd7UW6qaXLpeIlwdiCxZjDoeY1idiCVTletUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPzl6NUOg{txO M3jRenNCVkeHUh?=
human MCF7 cell NYrFdG5HT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkixO|gh|ryP NHnIdWtUSU6JRWK=
human A431 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1[1cGlvcGmkaYTpc44hd2ZiaIXtZY4hSTR|MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPFU6ODJizszN MnSxV2FPT0WU
human BC-3 cell NFzYVIpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWTJcohq[mm2aX;uJI9nKGi3bXHuJGJENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLki3NFk1KM7:TR?= NVfKRWNWW0GQR1XS
human A427 cell M2KzUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NETPSZhKdmirYnn0bY9vKG:oIHj1cYFvKEF2MkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlg4PjFzIN88US=> MYrTRW5ITVJ?
human SW620 cell MmOxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3HNXGlvcGmkaYTpc44hd2ZiaIXtZY4hW1d4MkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlkzQDF4IN88US=> MoXwV2FPT0WU
human HOS cell NFfiUo1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MorSTY5pcWKrdHnvckBw\iCqdX3hckBJV1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkm3N|EzKM7:TR?= MWnTRW5ITVJ?
human MFH-ino cell M2S5b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXT4WGRYUW6qaXLpeIlwdiCxZjDoeY1idiCPRlitbY5wKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS57OE[1PEDPxE1? M1yxfXNCVkeHUh?=
human TE-12 cell NHe5SFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFXPNnBKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU46QTF{ODFOwG0> NF2wSVFUSU6JRWK=
human KU812 cell Mn7FS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVrpNGZPUW6qaXLpeIlwdiCxZjDoeY1idiCNVUixNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOTh5MTFOwG0> M{K5TnNCVkeHUh?=
human NCI-H2342 cell MonES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXTLe21lUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI{PDJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{LkG5NFU{KM7:TR?= MWDTRW5ITVJ?
human BC-1 cell NYO4TXpYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXu5N3BPUW6qaXLpeIlwdiCxZjDoeY1idiCEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk42OTZ3MTFOwG0> MYHTRW5ITVJ?
human TE-15 cell M2jsfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXLJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj61PVE5OSEQvF2= MV3TRW5ITVJ?
human T84 cell MlTOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NU\KcIlvUW6qaXLpeIlwdiCxZjDoeY1idiCWOESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlYxPDR4IN88US=> M2\3enNCVkeHUh?=
human SW1710 cell M1zzTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfCb5VTUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjdyMEiyJO69VQ>? NWi5c5pRW0GQR1XS
human CAL-12T cell NUjVd|VWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkjBTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk44OTF3ODFOwG0> M1LhNXNCVkeHUh?=
human NKM-1 cell NYDMW4dwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3zjNWlvcGmkaYTpc44hd2ZiaIXtZY4hVkuPLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlc3PzNzIN88US=> MVvTRW5ITVJ?
human OCUB-M cell NGnO[GhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1TCV2lvcGmkaYTpc44hd2ZiaIXtZY4hV0OXQj3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk44QDh4MTFOwG0> NV7vb3dIW0GQR1XS
human 786-0 cell NWr4eFhWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVPSbYV5UW6qaXLpeIlwdiCxZjDoeY1idiB5OE[tNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTR6MkWg{txO MnzpV2FPT0WU
human EW-13 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGVYNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6wNVU2QCEQvF2= MmDVV2FPT0WU
human 5637 cell M3;C[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXfJcohq[mm2aX;uJI9nKGi3bXHuJFU3OzdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkKwOlY6KM7:TR?= M3PsV3NCVkeHUh?=
human PA-1 cell NYn4R3VVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJHBCNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkOzNFg{KM7:TR?= M2LzTHNCVkeHUh?=
human NCI-H2122 cell M4fOXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{jZTGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNVIzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5|M{Og{txO NF7QV29USU6JRWK=
human NCI-H460 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFLUdZVKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFYxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy52MESxJO69VQ>? M4jYbXNCVkeHUh?=
human CHL-1 cell NFv4eGZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2Poe2lvcGmkaYTpc44hd2ZiaIXtZY4hS0iOLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlQ6PjZ3IN88US=> Mn;RV2FPT0WU
human MOLT-16 cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFfQRpRKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjVzNUW1JO69VQ>? Mn\HV2FPT0WU
human HCT-15 cell MoD1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{L5[WlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUG1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{43Ojh2MjFOwG0> M1X6WnNCVkeHUh?=
human G-361 cell NG\ROYdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHviPYlKdmirYnn0bY9vKG:oIHj1cYFvKEdvM{[xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{44OTd2NTFOwG0> Moe3V2FPT0WU
human SK-LU-1 cell NGTNbmZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3rsXGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTGWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvQTdyMUSg{txO M{TKbHNCVkeHUh?=
human ES4 cell M2LH[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYPI[II{UW6qaXLpeIlwdiCxZjDoeY1idiCHU{SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlA3ODZ7IN88US=> NH24NlZUSU6JRWK=
human TE-8 cell NIHjR2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2nVS2lvcGmkaYTpc44hd2ZiaIXtZY4hXEVvODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNVE2QDJizszN MYDTRW5ITVJ?
human IGR-1 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mom2TY5pcWKrdHnvckBw\iCqdX3hckBKT1JvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNlM{KM7:TR?= NECx[JJUSU6JRWK=
human SW954 cell NHXido1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17uT2lvcGmkaYTpc44hd2ZiaIXtZY4hW1d7NUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlQ3OTJ2IN88US=> M1vaNHNCVkeHUh?=
human A375 cell MnjzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1j5cmlvcGmkaYTpc44hd2ZiaIXtZY4hSTN5NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNlU1QTJizszN NHPheWxUSU6JRWK=
human CAL-27 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXLJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|MzOjFizszN Moi1V2FPT0WU
human CAL-51 cell NHHOZopIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUHJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|U2QDNizszN MmPtV2FPT0WU
human SK-LMS-1 cell NV62Xmw3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXTJcohq[mm2aX;uJI9nKGi3bXHuJHNMNUyPUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{QDJ3NTFOwG0> MkL2V2FPT0WU
human HT-1080 cell NVf3eXFPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrLfFB2UW6qaXLpeIlwdiCxZjDoeY1idiCKVD2xNFgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS52OUO0O{DPxE1? NEnPUHdUSU6JRWK=
human NCI-H1299 cell NUHveWFTT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXvJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUK5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTh3NUeg{txO MmXCV2FPT0WU
human HT-29 cell NFTocW9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NY\MdIg6UW6qaXLpeIlwdiCxZjDoeY1idiCKVD2yPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOjZ6NEOg{txO M4T5VHNCVkeHUh?=
human A388 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGE{QDhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15LkW5PVY2KM7:TR?= NW[welBUW0GQR1XS
human SK-MEL-30 cell M1PXbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3fJcmlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVMxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py57N{KxOUDPxE1? MWnTRW5ITVJ?
human CMK cell M2fz[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH\vRmZKdmirYnn0bY9vKG:oIHj1cYFvKEOPSzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlU{PzdizszN NVnjboZHW0GQR1XS
human HuP-T4 cell MkfvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NETEOlNKdmirYnn0bY9vKG:oIHj1cYFvKEi3UD3UOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPTB{M{Sg{txO NGHQT29USU6JRWK=
human SF126 cell NXPK[XlZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NESyN45KdmirYnn0bY9vKG:oIHj1cYFvKFOIMUK2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE45ODJyODFOwG0> NGDBWXJUSU6JRWK=
human MKN1 cell NWK3cIJxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJG1MVjFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkG4OVY2KM7:TR?= MoLTV2FPT0WU
human LoVo cell MmDCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUiyR3hkUW6qaXLpeIlwdiCxZjDoeY1idiCOb2\vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU46OTJ7MjFOwG0> MWLTRW5ITVJ?
human HEL cell NGO2dZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NW\iWpBrUW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE43QCEQvF2= NX;LPVdRW0GQR1XS
human SW872 cell MoXNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mm\QTY5pcWKrdHnvckBw\iCqdX3hckBUXzh5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlY5QTZizszN NEf5dFZUSU6JRWK=
human SW982 cell M16z[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWmyV457UW6qaXLpeIlwdiCxZjDoeY1idiCVV{m4NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njd7MU[g{txO NF;x[XlUSU6JRWK=
human HuH-7 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MojITY5pcWKrdHnvckBw\iCqdX3hckBJfUhvNzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlI4QDNizszN MYHTRW5ITVJ?
human DU-145 cell M2rVbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MofaTY5pcWKrdHnvckBw\iCqdX3hckBFXS1zNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNE41QTV3IN88US=> NGnP[IhUSU6JRWK=
human MC116 cell NWP4O2pwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX23fpU3UW6qaXLpeIlwdiCxZjDoeY1idiCPQ{GxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjJ4N{[g{txO MYHTRW5ITVJ?
human H4 cell NVXwcWtDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MljCTY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJzLkSyOFQh|ryP M3LCNnNCVkeHUh?=
human SK-MEL-3 cell NVvkTZp4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGjMRYxKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzBwMEKyPEDPxE1? M2rkfXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p21 / LC3 / p62 / PARP / Cleaved PARP ; 

PubMed: 29017179     


PC3 cells were treated as in b; LNCaP cells were treated with DMSO or 80 μm GSK650394 (GSK) for various time points (6, 24 and 48 h). Total protein lysates were collected for western blot against p21, LC3B, p62, PARP and cleaved (CL) PARP; GAPDH was used as a loading control.

c-Jun / NRF2 ; 

PubMed: 31136958     


Western blot analysis of c-JUN and NRF2 proteins in cervical cancer cells treated with or without GSK650394. Vinculin was used as a loading control. 

p-SGK1 / SGK1 ; 

PubMed: 30873046     


Podocytes were exposed to 1, 2.5, 5, 10, or 20 μM GSK650394 for 24 h and then analyzed by immunoblot.

p-SGK2 / SGK2 ; 

PubMed: 26392083     


Western blot analysis of immunoprecipitated p-SGK2 T193 and FLAG-SGK2 from whole cell lysates in pcDNA/FLAG/SGK2-transfected ShP51 cells treated with GSK650394 (10 μM) for 30 and 60 min.

29017179 31136958 30873046 26392083
Immunofluorescence
LC3; 

PubMed: 29017179     


Immunofluorescence staining for LC3 puncta.

29017179
Growth inhibition assay
Cell viability; 

PubMed: 29017179     


Cell viability analysis using a CCK8 assay of prostate cancer cell lines after treatment with various concentrations of GSK650394 or vehicle alone for the indicated time periods. Data are the means of triplicate experiments. The results are expressed as the percentage of viable control cells treated with DMSO alone (vehicle). 

29017179

Protocol

Kinase Assay:[1]
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Scintillation proximity assay (SPA):

SGK1 S422D (60–431 aa; 0.275 μg/mL final concentration) or SGK2 (0.875 μg/mL final concentration) are activated by PDK1 (1.1 μg/mL final concentration) in a buffer consisting of 50 mM Tris (pH 7.5), 0.1 mM EGTA, 0.1 mM EDTA, 10 mM MgCl2, 0.1% β-mercaptoethanol, 1 mg/mL BSA, and ATP (final concentration of 0.15 mM) and incubated for 30 min at 30°C. SGK2 is prepared exactly as described for SGK1, except it corresponded to the full-length protein. A solution containing biotinylated CROSStide peptide at a final concentration of 75 μM and γ32P-ATP corresponding to 2×106 cpm is prepared in the reaction buffer. In a 96-well plate, 5 μL of GSK650394 is added to 25 μL of the activated enzyme mixture. To this, 20 μL of the CROSStide mixture is added and incubated for 1 h at room temperature. Next, 50 μL of a 25 mg/mL slurry of streptavidin-coated SPA beads in PBS with 0.1 M EDTA, pH 8.0 is added. The plate is then sealed and centrifuged for 8 min at 2000 rpm, and the signal is detected by measuring for 30 sec/well in a Packard TopCount NXT Scintillation Counter. The IC50 values of the inhibition of SGK1 and SGK2 activities by GSK650394 are calculated from these data using GraphPad Prism 3 Software.
Cell Research:[1]
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  • Cell lines: LNCaP cells
  • Concentrations: ~10 μM
  • Incubation Time: 7 days
  • Method: LNCaP cells are plated at a density of 5,000 cells per well in 96-well plates in 100 μL PRF-RPMI 1640, supplemented with 8% CS-FBS, 0.1 mM NEAA, and 1 mM NaPyr. At day three, cells are treated with hormone with or without GSK650394 by removing 50 μL of the media and replacing this with 50 μL of PRF-RPMI 1640 with 8% CS-FBS, NEAA, NaPyr containing a 2X concentration of the appropriate hormone/inhibitor treatment. At days 5 and 7, the treatment is repeated. On the tenth day, the media is removed and the relative cell number is measured using the FluoReporter Blue assay according to the manufacturer’s instructions.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 76 mg/mL (198.71 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 382.45
Formula

C25H22N2O2

CAS No. 890842-28-1
Storage powder
in solvent
Synonyms N/A
Smiles C1CCC(C1)C2=C(C=CC(=C2)C3=CNC4=C3C=C(C=N4)C5=CC=CC=C5)C(=O)O

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID