Glucose Transporter GLUT2 Rabbit mAb

Catalog No.: F2776

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000-1:10000

Application
WB

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
57 kDa 57 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Glucose Transporter GLUT2 Rabbit mAb detects endogenous levels of total Glut2 protein.

Clone
E14F15

Synonym(s)
GLUT2, SLC2A2, GLUT-2

Background
Glucose Transporter GLUT2, encoded by the SLC2A2 gene on chromosome 3q26.2, is a low-affinity, high-capacity facilitative glucose transporter belonging to the glucose transporter (GLUT) family. Structurally, it is a membrane glycoprotein with 14 transmembrane domains that mediates bidirectional, passive glucose transport to equilibrate glucose concentrations across membranes. GLUT2 is primarily expressed in the liver, pancreatic β cells, small intestine, kidney, and certain central nervous system regions. Functionally, it plays a pivotal role in glucose homeostasis by enabling glucose uptake for metabolism, sensing blood glucose levels (particularly in β cells and the CNS), mediating intestinal glucose/fructose absorption, hepatic glucose/fructose clearance, and renal glucose reabsorption. It suits for handling postprandial glucose surges, and variations in GLUT2 are linked to metabolic disorders such as type 2 diabetes, Fanconi–Bickel syndrome, and altered sugar preference.

Background

Glucose Transporter GLUT2, encoded by the SLC2A2 gene on chromosome 3q26.2, is a low-affinity, high-capacity facilitative glucose transporter belonging to the glucose transporter (GLUT) family. Structurally, it is a membrane glycoprotein with 14 transmembrane domains that mediates bidirectional, passive glucose transport to equilibrate glucose concentrations across membranes. GLUT2 is primarily expressed in the liver, pancreatic β cells, small intestine, kidney, and certain central nervous system regions. Functionally, it plays a pivotal role in glucose homeostasis by enabling glucose uptake for metabolism, sensing blood glucose levels (particularly in β cells and the CNS), mediating intestinal glucose/fructose absorption, hepatic glucose/fructose clearance, and renal glucose reabsorption. It suits for handling postprandial glucose surges, and variations in GLUT2 are linked to metabolic disorders such as type 2 diabetes, Fanconi–Bickel syndrome, and altered sugar preference.

References
https://pubmed.ncbi.nlm.nih.gov/37358764/ https://pubmed.ncbi.nlm.nih.gov/32591905/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%