Galectin 8/Gal-8 Rabbit mAb

Catalog No.: F2772

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100 IHC1:100 - 1:250 IF1:50 - 1:100 FCM1:10 - 1:100

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
40 kDa 36 kDa, 40 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Galectin 8/Gal-8 Rabbit mAb detects endogenous levels of total Galectin 8/Gal-8 protein.

Clone
G6M19

Synonym(s)
Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8

Background
Galectin 8/Gal-8 is a tandem-repeat-type β-galactoside-binding lectin widely expressed in human tissues and involved in diverse physiological and pathological processes. It contains two distinct carbohydrate recognition domains (N-CRD and C-CRD) connected by a linker peptide of variable length (33 aa in Gal-8S, 75 aa in Gal-8L), with the N-CRD uniquely showing high affinity for α2–3-sialylated or 3′-sulfated β-galactosides, and the C-CRD preferentially binding branched N-glycans. Alternative mRNA splicing generates multiple isoforms, that differs in CRD specificity and potential interdomain interactions. Functionally, Gal-8 acts as a matricellular protein regulating cell adhesion, migration, and immune responses, displaying both immunosuppressive and immunostimulatory effects. It plays roles in tumor biology, autoimmune and inflammatory diseases, osteoclastogenesis, and tissue morphogenesis, with expression patterns in various cancers suggesting potential diagnostic and prognostic applications.

Background

Galectin 8/Gal-8 is a tandem-repeat-type β-galactoside-binding lectin widely expressed in human tissues and involved in diverse physiological and pathological processes. It contains two distinct carbohydrate recognition domains (N-CRD and C-CRD) connected by a linker peptide of variable length (33 aa in Gal-8S, 75 aa in Gal-8L), with the N-CRD uniquely showing high affinity for α2–3-sialylated or 3′-sulfated β-galactosides, and the C-CRD preferentially binding branched N-glycans. Alternative mRNA splicing generates multiple isoforms, that differs in CRD specificity and potential interdomain interactions. Functionally, Gal-8 acts as a matricellular protein regulating cell adhesion, migration, and immune responses, displaying both immunosuppressive and immunostimulatory effects. It plays roles in tumor biology, autoimmune and inflammatory diseases, osteoclastogenesis, and tissue morphogenesis, with expression patterns in various cancers suggesting potential diagnostic and prognostic applications.

References
https://pubmed.ncbi.nlm.nih.gov/29934665/ https://pubmed.ncbi.nlm.nih.gov/24696431/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%