FoxC2 Rabbit mAb

Catalog No.: F1331

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
60 kDa

Datasheet & SDS

Biological Description

Specificity
FoxC2 Rabbit mAb recognizes endogenous levels of total FoxC2 protein.

Clone
C7N1

Synonym(s)
Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1 (MFH-1 protein), Transcription factor FKH-14, FOXC2, FKHL14, MFH1

Background
FOXC2 is a member of the forkhead box (FOX) family of transcription factors that plays an essential role in the regulation of vascular and lymphatic development, as well as in processes like epithelial-mesenchymal transition (EMT), which is critical for cancer metastasis. FOXC2 contains a conserved winged-helix DNA-binding domain (DBD) responsible for its sequence-specific binding to DNA, primarily recognizing the motif (T/C)AAACN through helix 3 interactions with the DNA major groove, supported by auxiliary contacts from the N-terminus and wing domains that stabilize the complex. Key residues including Asn118, Arg121, His122, and Ser125 within helix 3, mediate these specific DNA contacts, while the C-terminal region and wing domains mainly interact with the phosphate backbone to stabilize binding. FOXC2 regulates transcriptional programs involved in angiogenesis, lymphangiogenesis, cell proliferation, and EMT, thereby influencing vascular remodeling, tumor progression, and metastasis. Mutations or dysregulation of FOXC2 are linked to developmental disorders such as lymphedema-distichiasis syndrome, as well as to cancer aggressiveness through enhancement of invasive phenotypes.

Background

FOXC2 is a member of the forkhead box (FOX) family of transcription factors that plays an essential role in the regulation of vascular and lymphatic development, as well as in processes like epithelial-mesenchymal transition (EMT), which is critical for cancer metastasis. FOXC2 contains a conserved winged-helix DNA-binding domain (DBD) responsible for its sequence-specific binding to DNA, primarily recognizing the motif (T/C)AAACN through helix 3 interactions with the DNA major groove, supported by auxiliary contacts from the N-terminus and wing domains that stabilize the complex. Key residues including Asn118, Arg121, His122, and Ser125 within helix 3, mediate these specific DNA contacts, while the C-terminal region and wing domains mainly interact with the phosphate backbone to stabilize binding. FOXC2 regulates transcriptional programs involved in angiogenesis, lymphangiogenesis, cell proliferation, and EMT, thereby influencing vascular remodeling, tumor progression, and metastasis. Mutations or dysregulation of FOXC2 are linked to developmental disorders such as lymphedema-distichiasis syndrome, as well as to cancer aggressiveness through enhancement of invasive phenotypes.

References
https://pubmed.ncbi.nlm.nih.gov/36740986/ https://pubmed.ncbi.nlm.nih.gov/31460188/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%