Datasheet

Biological Description

Specificity	FoxC2 Rabbit mAb recognizes endogenous levels of total FoxC2 protein.
Background	FOXC2 is a member of the forkhead box (FOX) family of transcription factors that plays an essential role in the regulation of vascular and lymphatic development, as well as in processes like epithelial-mesenchymal transition (EMT), which is critical for cancer metastasis. FOXC2 contains a conserved winged-helix DNA-binding domain (DBD) responsible for its sequence-specific binding to DNA, primarily recognizing the motif (T/C)AAACN through helix 3 interactions with the DNA major groove, supported by auxiliary contacts from the N-terminus and wing domains that stabilize the complex. Key residues including Asn118, Arg121, His122, and Ser125 within helix 3, mediate these specific DNA contacts, while the C-terminal region and wing domains mainly interact with the phosphate backbone to stabilize binding. FOXC2 regulates transcriptional programs involved in angiogenesis, lymphangiogenesis, cell proliferation, and EMT, thereby influencing vascular remodeling, tumor progression, and metastasis. Mutations or dysregulation of FOXC2 are linked to developmental disorders such as lymphedema-distichiasis syndrome, as well as to cancer aggressiveness through enhancement of invasive phenotypes.

Specificity

FoxC2 Rabbit mAb recognizes endogenous levels of total FoxC2 protein.

Background

FOXC2 is a member of the forkhead box (FOX) family of transcription factors that plays an essential role in the regulation of vascular and lymphatic development, as well as in processes like epithelial-mesenchymal transition (EMT), which is critical for cancer metastasis. FOXC2 contains a conserved winged-helix DNA-binding domain (DBD) responsible for its sequence-specific binding to DNA, primarily recognizing the motif (T/C)AAACN through helix 3 interactions with the DNA major groove, supported by auxiliary contacts from the N-terminus and wing domains that stabilize the complex. Key residues including Asn118, Arg121, His122, and Ser125 within helix 3, mediate these specific DNA contacts, while the C-terminal region and wing domains mainly interact with the phosphate backbone to stabilize binding. FOXC2 regulates transcriptional programs involved in angiogenesis, lymphangiogenesis, cell proliferation, and EMT, thereby influencing vascular remodeling, tumor progression, and metastasis. Mutations or dysregulation of FOXC2 are linked to developmental disorders such as lymphedema-distichiasis syndrome, as well as to cancer aggressiveness through enhancement of invasive phenotypes.

Usage Information

Application

WB

Dilution

WB

1:1000

Reactivity

Human

Source

Rabbit

MW

60 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/36740986/
https://pubmed.ncbi.nlm.nih.gov/31460188/

FoxC2 Rabbit mAb

Biological Description

Usage Information

References

Application Data