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Flotillin-1 (J14F12) Rabbit mAb

Catalog No.: F3788

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:10000 - 1:50000 IP1:10 - 1:100 IHC1:100 - 1:250 IF1:500 FCM1:50

Application
WB, IP, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
47 kDa 50 kDa, 48 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Flotillin-1 (J14F12) Rabbit mAb detects endogenous levels of total Flotillin-1 protein.

Clone
J14F12

Synonym(s)
Flotillin-1, FLOT1

Background
Flotillin 1 is a conserved membrane-associated protein of the SPFH (Stomatin, Prohibitin, Flotillin, HflK/C) superfamily that functions as a core scaffolding component of cholesterol-rich lipid rafts, where it forms hetero‑oligomeric complexes with Flotillin 2 to organize membrane architecture and signaling platforms. It contains an N‑terminal SPFH domain that anchors to the inner plasma membrane and mediates oligomerization, and a C‑terminal flotillin domain that stabilizes higher‑order structures through coiled‑coil and β‑strand interactions. Flotillin 1 regulates clathrin‑independent endocytosis, receptor clustering, insulin and immune receptor signaling, cytoskeletal organization, vesicle trafficking, and lateral membrane compartmentalization, also localizing to endosomes, exosomes, lysosomes, and sometimes the nucleus. By inducing membrane curvature and providing stable nanodomains, it coordinates pathways governing cell adhesion, motility, and signal transduction. Dysregulated Flotillin 1 is associated with cancer progression by promoting metastasis through modulation of oncogenic signaling, and with neurodegenerative and metabolic disorders through disturbances in membrane organization and intracellular trafficking.

Background

Flotillin 1 is a conserved membrane-associated protein of the SPFH (Stomatin, Prohibitin, Flotillin, HflK/C) superfamily that functions as a core scaffolding component of cholesterol-rich lipid rafts, where it forms hetero‑oligomeric complexes with Flotillin 2 to organize membrane architecture and signaling platforms. It contains an N‑terminal SPFH domain that anchors to the inner plasma membrane and mediates oligomerization, and a C‑terminal flotillin domain that stabilizes higher‑order structures through coiled‑coil and β‑strand interactions. Flotillin 1 regulates clathrin‑independent endocytosis, receptor clustering, insulin and immune receptor signaling, cytoskeletal organization, vesicle trafficking, and lateral membrane compartmentalization, also localizing to endosomes, exosomes, lysosomes, and sometimes the nucleus. By inducing membrane curvature and providing stable nanodomains, it coordinates pathways governing cell adhesion, motility, and signal transduction. Dysregulated Flotillin 1 is associated with cancer progression by promoting metastasis through modulation of oncogenic signaling, and with neurodegenerative and metabolic disorders through disturbances in membrane organization and intracellular trafficking.

References
https://pubmed.ncbi.nlm.nih.gov/38985763/ https://pubmed.ncbi.nlm.nih.gov/34285255/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%