Name: Corticosterone
Brand: Selleck Chemicals
SKU: S4752
Availability: InStock
Rating: 5 (8 reviews)

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Quality Control

Batch: Purity: 99.95%

99.95

Related Targets	Adrenergic Receptor Estrogen/progestogen Receptor GPR Androgen Receptor ACE RAAS Progesterone Receptor Opioid Receptor PGES THR
Other Glucocorticoid Receptor Products	AL082D06 (20S)-Protopanaxatriol Glucocorticoid Receptor Antibody [D9N22] Cortodoxone

Solubility

In vitro
Batch:

DMSO : 69 mg/mL (199.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 69 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (14.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.44mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	346.46	Formula	C₂₁H₃₀O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	50-22-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Mechanism of Action

Targets/IC₅₀/Ki	Glucocorticoid receptor
In vitro	Corticosterone, via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane. It enhances AMPAR-mediated miniature excitatory postsynaptic current (mEPSC) amplitude and surface expression of GluR1 and GluR2 subunits in hippocampal neurons, increases the surface mobility and synaptic content of AMPAR GluR2 subunits, and enhances L-type calcium currents in CA1 pyramidal neurons. This compound operates through mineralocorticoid receptors and glucocorticoid receptors, both of which belong to the family of nuclear receptors that bind to response elements in the DNA, thus modifying the activity of responsive genes.
In vivo	Corticosterone plays an important role in regulating neuronal functions of the limbic system. After stress, the level of stress hormones such as this compound is markedly increased. It exerts a time- and region-specific action on cellular physiology of limbic neurons.
References	[1] https://pubmed.ncbi.nlm.nih.gov/20051515/

Applications

Methods	Biomarkers	Images	PMID
Growth inhibition assay	Cell viability		30037364

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05903716	Recruiting	Acne Vulgaris	Marmara University	September 15 2022	--
NCT04930471	Unknown status	Autistic Disorder	Insel Gruppe AG University Hospital Bern\|Lindenhofstiftung	June 2021	--
NCT02826278	Unknown status	Newborn Genitalia	Hospices Civils de Lyon	October 2014	--
NCT01666314	Completed	Prostate Cancer	Millennium Pharmaceuticals Inc.\|Takeda	August 20 2012	Phase 1\|Phase 2
NCT01186484	Completed	Prostatic Neoplasms	Janssen Pharmaceutical K.K.	June 1 2010	Phase 1

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Corticosterone Glucocorticoid Receptor agonist

Chemical Structure

Molecular Weight: 346.46