Corticosterone (NSC-9705)

Catalog No.S4752 Synonyms: 17-deoxycortisol, 11β,21-dihydroxyprogesterone

For research use only.

Corticosterone (NSC-9705, 17-deoxycortisol, 11β,21-dihydroxyprogesterone), the major stress hormone, is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid.

Corticosterone (NSC-9705) Chemical Structure

CAS No. 50-22-6

Selleck's Corticosterone (NSC-9705) has been cited by 3 Publications

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Biological Activity

Description Corticosterone (NSC-9705, 17-deoxycortisol, 11β,21-dihydroxyprogesterone), the major stress hormone, is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid.
Targets
Glucocorticoid receptor [2]
()
In vitro

Corticosterone, via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane. It enhances AMPAR-mediated miniature excitatory postsynaptic current (mEPSC) amplitude and surface expression of GluR1 and GluR2 subunits in hippocampal neurons, increases the surface mobility and synaptic content of AMPAR GluR2 subunits, and enhances L-type calcium currents in CA1 pyramidal neurons. Corticosterone operates through mineralocorticoid receptors and glucocorticoid receptors, both of which belong to the family of nuclear receptors that bind to response elements in the DNA, thus modifying the activity of responsive genes[1].

Assay
Methods Test Index PMID
Growth inhibition assay Cell viability 30037364
In vivo Corticosterone plays an important role in regulating neuronal functions of the limbic system. After stress, the level of stress hormones such as corticosterone is markedly increased. Corticosterone exerts a time- and region-specific action on cellular physiology of limbic neurons[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HEK293 cells
  • Concentrations: 100 nM
  • Incubation Time: 30 min
  • Method: HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent, the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis, the following approach is used. HEK293 cells are transfected with or without SGK1 small interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min. The supernatants (40 μl, ∼50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 69 mg/mL
(199.15 mM)
Ethanol 69 mg/mL warmed
(199.15 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 346.46
Formula

C21H30O4

CAS No. 50-22-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4C(=O)CO)C)O

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04930471 Not yet recruiting Drug: Metformin Autistic Disorder University Hospital Inselspital Berne|Lindenhofstiftung June 2021 --
NCT02826278 Unknown status Other: External genitalia measurements in female newborns Newborn Genitalia Hospices Civils de Lyon October 2014 --
NCT01666314 Completed Drug: Orteronel|Drug: Orteronel Placebo|Drug: Prednisone Prostate Cancer Millennium Pharmaceuticals Inc.|Takeda August 20 2012 Phase 1|Phase 2
NCT01186484 Completed Drug: JNJ-212082 Prostatic Neoplasms Janssen Pharmaceutical K.K. June 1 2010 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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