Home PI3K/Akt/mTOR PTEN inhibitor bpV (HOpic)

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bpV (HOpic) PTEN inhibitor

Cat.No.S8651

bpV (HOpic) (Bisperoxovanadium (HOpic)) is a potent inhibitor of PTEN with an IC50 of 14 nM. The IC50s for PTP-β and PTP-1B are about 350- and 1800-fold higher than that for PTEN, respectively.
bpV (HOpic) PTEN inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 347.24

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Quality Control

Batch: S865101 Water]69 mg/mL]false]DMSO]Insoluble]false]Ethanol]Insoluble]false Purity: 99.53%
99.53

Chemical Information, Storage & Stability

Molecular Weight 347.24 Formula

C6H4NO8V.2K

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 722494-26-0 -- Storage of Stock Solutions

Synonyms Bisperoxovanadium (HOpic) Smiles [K+].[K+].OC1=CC=C2N(=C1)|[V+3]|3|4(|[O-][O-]|3)(|[O-][O-]|4)(|[O-]C2=O)=O

Solubility

In vitro
Batch:

Water : 69 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Mechanism of Action

Targets/IC50/Ki
PTEN [1]
(Cell-free assay)
14 nM
In vitro
Treatment with 1 μM BpV(HOpic) is capable of increasing the in vitro migration of C2C12 myoblasts without significantly reducing their ability to differentiate and fuse into multinucleated myotubes. This compound enhances AKT and ERK1/2 signaling, which is in conjunction with its effects on myoblast migration[2].
In vivo
Compared with vehicle-treated mice with IRI (renal ischemia/reperfusion injury), pharmacological inhibition of PTEN with bpV (HOpic) exacerbates renal dysfunction and promotes tubular damage. PTEN inhibition with this compound enhances tubular cell apoptosis in kidneys with IRI, which is associated with excessive caspase-3 activation. Furthermore, it expands the infiltration of neutrophils and macrophages into kidneys with IRI, which is accompanied by increased expression of the proinflammatory molecules[3].
References

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