bpV (HOpic)

Catalog No.S8651 Synonyms: Bisperoxovanadium (HOpic)

For research use only.

bpV (HOpic) (Bisperoxovanadium (HOpic)) is a potent inhibitor of PTEN with an IC50 of 14 nM. The IC50s for PTP-β and PTP-1B are about 350- and 1800-fold higher than the IC50 for PTEN, respectively.

bpV (HOpic) Chemical Structure

CAS No. 722494-26-0

Selleck's bpV (HOpic) has been cited by 9 Publications

Purity & Quality Control

Choose Selective PTEN Inhibitors

Biological Activity

Description bpV (HOpic) (Bisperoxovanadium (HOpic)) is a potent inhibitor of PTEN with an IC50 of 14 nM. The IC50s for PTP-β and PTP-1B are about 350- and 1800-fold higher than the IC50 for PTEN, respectively.
Targets
PTEN [1]
(Cell-free assay)
14 nM
In vitro

1 μM BpV(Hopic) treatment is capable of increasing the in vitro migration of C2C12 myoblasts, without significantly reducing their ability to differentiate and fuse into multinucleated myotubes. The effects on myoblast migration is in conjuction with enhanced AKT and ERK1/2 signaling[2].

In vivo Pharmacological inhibition of PTEN with bpV(HOpic) exacerbates renal dysfunction and promotes tubular damage in mice with IRI(renal ischemia/reperfusion injury) compared with vehicle-treated mice with IRI. PTEN inhibition enhances tubular cell apoptosis in kidneys with IRI, which is associated with excessive caspase-3 activation. Furthermore, PTEN inhibition expands the infiltration of neutrophils and macrophages into kidneys with IRI, which is accompanied by increased expression of the proinflammatory molecules[3].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: C2C12 myoblasts
  • Concentrations: 1 μM or 10 μM
  • Incubation Time: 72 h
  • Method:

    C2C12 myoblasts are grown to high confluency and triggered to differentiate in the presence of DM, supplemented with 1 μM or 10 μM BpV(Hopic).

Animal Research:

[3]

  • Animal Models: mouse model of ischemia-reperfusion injury (C57BL/6 mice, 8-12 weeks old)
  • Dosages: 200 μg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Water 69 mg/mL
(198.7 mM)
DMSO Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 347.24
Formula

C6H4NO8V.2K

CAS No. 722494-26-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles [K+].[K+].OC1=CC=C2N(=C1)|[V+3]|3|4(|[O-][O-]|3)(|[O-][O-]|4)(|[O-]C2=O)=O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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