ARPC5/p16 ARC Rabbit mAb

Catalog No.: F2810

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000-1:10000 IP1:20 - 1:70 IHC1:50 IF1:50-1:100

Application
WB, IP, IHC, IF

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
16 kDa 16 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
ARPC5/p16 ARC Rabbit mAb detects endogenous levels of total ARPC5/p16 ARC protein.

Clone
G16G3

Synonym(s)
ARC16, ARPC5, Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC

Background
ARPC5/p16 ARC is a protein that serves as one of the seven subunits of the ubiquitously expressed actin-related protein 2/3 (Arp2/3) complex, a key actin nucleator responsible for generating branched actin filament networks essential for diverse cellular processes such as cell migration, vesicle trafficking, receptor endocytosis, and immune synapse formation. Structurally, ARPC5/p16 ARC is a small, predominantly α-helical subunit that helps stabilize the Arp2/3 complex and modulates its actin-nucleating efficiency, with mammals expressing two paralogous isoforms—ARPC5 and ARPC5L. ARPC5/p16 ARC is ubiquitously expressed, with detectable levels in immune cells, muscle, and neural tissues, and is differentially required for specific actin-dependent processes, such as organizing transverse triads in muscle and regulating immune cell activation. Functionally, ARPC5/p16 ARC contributes to branched actin assembly, cell polarity, and intracellular trafficking, and its loss impairs cytoskeletal organization, cytokine signaling (notably IL-6), and immune responses. Pathogenic biallelic ARPC5/p16 ARC mutations cause severe developmental defects, prenatal lethality in mice, and syndromic early-onset immunodeficiency in humans, while aberrant overexpression is linked to cancer progression, invasion, and poor prognosis in tumors such as gliomas and hepatocellular carcinoma.

Background

ARPC5/p16 ARC is a protein that serves as one of the seven subunits of the ubiquitously expressed actin-related protein 2/3 (Arp2/3) complex, a key actin nucleator responsible for generating branched actin filament networks essential for diverse cellular processes such as cell migration, vesicle trafficking, receptor endocytosis, and immune synapse formation. Structurally, ARPC5/p16 ARC is a small, predominantly α-helical subunit that helps stabilize the Arp2/3 complex and modulates its actin-nucleating efficiency, with mammals expressing two paralogous isoforms—ARPC5 and ARPC5L. ARPC5/p16 ARC is ubiquitously expressed, with detectable levels in immune cells, muscle, and neural tissues, and is differentially required for specific actin-dependent processes, such as organizing transverse triads in muscle and regulating immune cell activation. Functionally, ARPC5/p16 ARC contributes to branched actin assembly, cell polarity, and intracellular trafficking, and its loss impairs cytoskeletal organization, cytokine signaling (notably IL-6), and immune responses. Pathogenic biallelic ARPC5/p16 ARC mutations cause severe developmental defects, prenatal lethality in mice, and syndromic early-onset immunodeficiency in humans, while aberrant overexpression is linked to cancer progression, invasion, and poor prognosis in tumors such as gliomas and hepatocellular carcinoma.

References
[1] Ming Y, et al. BMC Cancer. 2023 Oct 3;23(1):937. Sindram E, Caballero-Oteyza A, Kogata N, Chor Mei Huang S, Alizadeh Z, Gámez-Díaz L, Fazlollhi MR, Peng X, Grimbacher B, Way M, Proietti M. ARPC5 deficiency leads to severe early-onset systemic inflammation and mortality. Dis Model Mech. 2023 Jul 1;16(7):dmm050145.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%