ARPC5/p16 ARC Rabbit mAb

Catalog No.: F2810

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Biological Description

Specificity ARPC5/p16 ARC Rabbit mAb detects endogenous levels of total ARPC5/p16 ARC protein.
Background ARPC5/p16 ARC is a protein that serves as one of the seven subunits of the ubiquitously expressed actin-related protein 2/3 (Arp2/3) complex, a key actin nucleator responsible for generating branched actin filament networks essential for diverse cellular processes such as cell migration, vesicle trafficking, receptor endocytosis, and immune synapse formation. Structurally, ARPC5/p16 ARC is a small, predominantly α-helical subunit that helps stabilize the Arp2/3 complex and modulates its actin-nucleating efficiency, with mammals expressing two paralogous isoforms—ARPC5 and ARPC5L. ARPC5/p16 ARC is ubiquitously expressed, with detectable levels in immune cells, muscle, and neural tissues, and is differentially required for specific actin-dependent processes, such as organizing transverse triads in muscle and regulating immune cell activation. Functionally, ARPC5/p16 ARC contributes to branched actin assembly, cell polarity, and intracellular trafficking, and its loss impairs cytoskeletal organization, cytokine signaling (notably IL-6), and immune responses. Pathogenic biallelic ARPC5/p16 ARC mutations cause severe developmental defects, prenatal lethality in mice, and syndromic early-onset immunodeficiency in humans, while aberrant overexpression is linked to cancer progression, invasion, and poor prognosis in tumors such as gliomas and hepatocellular carcinoma.

Usage Information

Application WB, IP, IHC, IF Dilution
WB IP IHC IF
1:1000-1:10000 1:20 - 1:70 1:50 1:50-1:100
Reactivity Human, Mouse, Rat
Source Rabbit MW 16 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data