Aromatase Rabbit mAb

Catalog No.: F1389

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:50 IHC1:4200

Application
WB, IP, IHC

Source
Rabbit

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50 kDa

Datasheet & SDS

Biological Description

Specificity
Aromatase Rabbit mAb recognizes endogenous levels of total aromatase protein.

Clone
H2G4

Synonym(s)
Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1 1, Estrogen synthase, CYP19A1 , ARO1, CYAR, CYP19

Background
Aromatase is an essential enzyme responsible for converting androgens, such as testosterone and androstenedione, into estrogens like 17β-estradiol (E2) and estrone (E1) through a series of three hydroxylation reactions. As a member of the cytochrome P450 superfamily, aromatase contains a heme group and functions within the endoplasmic reticulum of estrogen-producing cells. For its catalytic activity, it requires NADPH-cytochrome P450 reductase (CPR) as an electron donor. Aromatase plays a critical role in estrogen biosynthesis and is a key therapeutic target in the treatment of hormone receptor-positive breast cancer. However, in endocrine-resistant cancer cells, its activity can be upregulated through signaling pathways such as PI3K/Akt and insulin-like growth factor receptor (IGFR). Additionally, phosphorylation has been shown to influence aromatase activity, indicating another layer of regulatory control. In humans, only a single aromatase protein exists, but its expression is tightly regulated and tissue-specific. It is predominantly expressed in the ovaries of premenopausal women, the placenta during pregnancy, and in adipose fibroblasts in postmenopausal women. In breast tissue, aromatase expression has been specifically identified in mesenchymal stromal cells (preadipocytes) as well as epithelial cells, highlighting its diverse role in local estrogen production.

Background

Aromatase is an essential enzyme responsible for converting androgens, such as testosterone and androstenedione, into estrogens like 17β-estradiol (E2) and estrone (E1) through a series of three hydroxylation reactions. As a member of the cytochrome P450 superfamily, aromatase contains a heme group and functions within the endoplasmic reticulum of estrogen-producing cells. For its catalytic activity, it requires NADPH-cytochrome P450 reductase (CPR) as an electron donor. Aromatase plays a critical role in estrogen biosynthesis and is a key therapeutic target in the treatment of hormone receptor-positive breast cancer. However, in endocrine-resistant cancer cells, its activity can be upregulated through signaling pathways such as PI3K/Akt and insulin-like growth factor receptor (IGFR). Additionally, phosphorylation has been shown to influence aromatase activity, indicating another layer of regulatory control. In humans, only a single aromatase protein exists, but its expression is tightly regulated and tissue-specific. It is predominantly expressed in the ovaries of premenopausal women, the placenta during pregnancy, and in adipose fibroblasts in postmenopausal women. In breast tissue, aromatase expression has been specifically identified in mesenchymal stromal cells (preadipocytes) as well as epithelial cells, highlighting its diverse role in local estrogen production.

References
[1] Chan HJ, et al. J Steroid Biochem Mol Biol. 2016 Jul:161:73-83.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%