Aromatase Rabbit mAb

Catalog No.: F1389

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Biological Description

Specificity Aromatase Rabbit mAb recognizes endogenous levels of total aromatase protein.
Background Aromatase is an essential enzyme responsible for converting androgens, such as testosterone and androstenedione, into estrogens like 17β-estradiol (E2) and estrone (E1) through a series of three hydroxylation reactions. As a member of the cytochrome P450 superfamily, aromatase contains a heme group and functions within the endoplasmic reticulum of estrogen-producing cells. For its catalytic activity, it requires NADPH-cytochrome P450 reductase (CPR) as an electron donor. Aromatase plays a critical role in estrogen biosynthesis and is a key therapeutic target in the treatment of hormone receptor-positive breast cancer. However, in endocrine-resistant cancer cells, its activity can be upregulated through signaling pathways such as PI3K/Akt and insulin-like growth factor receptor (IGFR). Additionally, phosphorylation has been shown to influence aromatase activity, indicating another layer of regulatory control. In humans, only a single aromatase protein exists, but its expression is tightly regulated and tissue-specific. It is predominantly expressed in the ovaries of premenopausal women, the placenta during pregnancy, and in adipose fibroblasts in postmenopausal women. In breast tissue, aromatase expression has been specifically identified in mesenchymal stromal cells (preadipocytes) as well as epithelial cells, highlighting its diverse role in local estrogen production.

Usage Information

Application WB, IP, IHC Dilution
WB IP IHC
1:1000 1:50 1:4200
Reactivity Human
Source Rabbit MW 50 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data