Catalog No.S8487 Synonyms: B/B Homodimerizer

For research use only.

AP20187 (B/B Homodimerizer) is a chemical inducer of dimerization that activates FKBP-Casp8.

AP20187 Chemical Structure

CAS No. 195514-80-8

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Biological Activity

Description AP20187 (B/B Homodimerizer) is a chemical inducer of dimerization that activates FKBP-Casp8.
FKBP [1] Casp8 [1]
In vitro

In oligodendrocytes, AP20187 treatment increases the expression of PERK-responsive genes encoding CAATT enhancer binding protein homologous protein (CHOP) and growth arrest and DNA damage 34 (GADD34) in a dose-dependent manner. The activity of PERK signaling in oligodendrocytes can be temporally manipulated by the administration of AP20187.[2]

In vivo

AP20187 treatment significantly reduces the number of degenerating axons and increases the density of axons in the demyelinating lesions in the lumbar spinal cord of PLP/Fv2E-PERK mice. AP20187 is also sufficient to activate Fv2E-PERK in oligodendrocytes in the CNS of PLP/Fv2E-PERK mice.[2]

Protocol (from reference)

Cell Research:


  • Cell lines: Oligodendrocytes
  • Concentrations: 1 nM
  • Incubation Time: 4 h
  • Method:

    After 3 d of differentiation, the cells are treated with 1 nM AP20187 or vehicle for 4 h and are then fixed with cold 4% paraformaldehyde in PBS. The cells are blocked with PBS containing 10% goat serum and 0.1% Triton X-100 and incubate overnight with the primary antibody diluted in blocking solution. Appropriate fluorochrome-labeled secondary antibodies are used for detection.

Animal Research:


  • Animal Models: PLP/Fv2E-PERK mice
  • Dosages: 0.5 mg/kg, 2 mg/kg, 5 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 1482.75


CAS No. 195514-80-8
Storage 3 years -20°C powder
2 years -80°C in solvent

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