Anti-Perforin Mouse Antibody [P20D17]

Catalog No.: F2444

Application: Reactivity: Human

Usage Information

Dilution
IHC1:100

Application
IHC

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
70 kDa

Positive Control	Human spleen tissue
Negative Control

Datasheet & SDS

Biological Description

Specificity
Perforin Mouse mAb recognizes endogenous levels of total Perforin protein.

Clone
P20D17

Synonym(s)
PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

Background
Perforin is a pore-forming glycoprotein mainly produced by cytotoxic lymphocytes such as natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). It belongs to the MACPF (membrane attack complex/perforin) family and consists of domains critical for its function: the MACPF domain responsible for pore formation, a calcium-dependent C2 domain mediating membrane binding, and an EGF-like domain providing structural flexibility. Once secreted into the immunological synapse formed between the cytotoxic cell and target (virus-infected or malignant) cell, perforin oligomerizes and inserts into the target cell membrane, forming transmembrane pores approximately 20 nm in diameter. These pores allow entry of pro-apoptotic granzymes into the cytosol, which trigger apoptosis of the target cell. Key residues such as R298 are important for oligomerization and pore formation. Perforin-mediated pore formation is essential for immune surveillance and elimination of abnormal cells, and deficiencies or mutations in perforin cause severe immunodeficiency disorders like familial hemophagocytic lymphohistiocytosis (FHL). Perforin also plays roles in immune regulation and is implicated when cytotoxic function is disrupted or dysregulated in various diseases.

Background

Perforin is a pore-forming glycoprotein mainly produced by cytotoxic lymphocytes such as natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). It belongs to the MACPF (membrane attack complex/perforin) family and consists of domains critical for its function: the MACPF domain responsible for pore formation, a calcium-dependent C2 domain mediating membrane binding, and an EGF-like domain providing structural flexibility. Once secreted into the immunological synapse formed between the cytotoxic cell and target (virus-infected or malignant) cell, perforin oligomerizes and inserts into the target cell membrane, forming transmembrane pores approximately 20 nm in diameter. These pores allow entry of pro-apoptotic granzymes into the cytosol, which trigger apoptosis of the target cell. Key residues such as R298 are important for oligomerization and pore formation. Perforin-mediated pore formation is essential for immune surveillance and elimination of abnormal cells, and deficiencies or mutations in perforin cause severe immunodeficiency disorders like familial hemophagocytic lymphohistiocytosis (FHL). Perforin also plays roles in immune regulation and is implicated when cytotoxic function is disrupted or dysregulated in various diseases.

References
https://pubmed.ncbi.nlm.nih.gov/20536554/ https://pubmed.ncbi.nlm.nih.gov/35148176/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%