Acetyl-Histone H2B (Lys5) Rabbit mAb

Catalog No.: F1271

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:100 IHC1:1000 IF1:400 CHIP1:50

Application
WB, IP, IHC, IF, ChIP

Source
Rabbit

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
14 kDa

Datasheet & SDS

Biological Description

Specificity
Acetyl-Histone H2B (Lys5) Rabbit mAb recognizes endogenous levels of histone H2B only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones.

Clone
B12A5

Synonym(s)
Histone H2B type 1-B, H2B-clustered histone 3, Histone H2B.1, Histone H2B.f (H2B/f), H2BC3, H2BFF, HIST1H2BB

Background
Histone H2B is one of the five main histone proteins, alongside H1/H5, H2A, H3, and H4. Within the nucleosome—the fundamental unit of chromatin—two molecules each of H2A, H2B, H3, and H4 assemble into an octameric core around which DNA wraps in a left-handed supercoil. Histones are subject to a range of post-translational modifications (PTMs) that play vital roles in regulating transcription, DNA replication and repair, and maintaining chromosomal integrity. During transcriptional activation, the histone acetyltransferases p300 and CBP catalyze the acetylation of multiple lysine residues on the N-terminal tail of histone H2B, particularly at Lys5, Lys12, Lys15, and Lys20. This acetylation reduces the positive charge on histone tails, thereby weakening their interactions with negatively charged DNA and between adjacent nucleosomes. As a result, chromatin becomes less compact and more accessible to transcription factors and other DNA-binding proteins. Furthermore, acetylation of specific lysine residues generates recognition sites for transcriptional co-regulators and chromatin remodeling factors that possess bromodomains—structural motifs that specifically bind to acetylated lysines—facilitating their recruitment to target gene promoters and enhancing gene expression.

Background

Histone H2B is one of the five main histone proteins, alongside H1/H5, H2A, H3, and H4. Within the nucleosome—the fundamental unit of chromatin—two molecules each of H2A, H2B, H3, and H4 assemble into an octameric core around which DNA wraps in a left-handed supercoil. Histones are subject to a range of post-translational modifications (PTMs) that play vital roles in regulating transcription, DNA replication and repair, and maintaining chromosomal integrity. During transcriptional activation, the histone acetyltransferases p300 and CBP catalyze the acetylation of multiple lysine residues on the N-terminal tail of histone H2B, particularly at Lys5, Lys12, Lys15, and Lys20. This acetylation reduces the positive charge on histone tails, thereby weakening their interactions with negatively charged DNA and between adjacent nucleosomes. As a result, chromatin becomes less compact and more accessible to transcription factors and other DNA-binding proteins. Furthermore, acetylation of specific lysine residues generates recognition sites for transcriptional co-regulators and chromatin remodeling factors that possess bromodomains—structural motifs that specifically bind to acetylated lysines—facilitating their recruitment to target gene promoters and enhancing gene expression.

References
[1] Peterson CL, et al. Curr Biol. 2004 Jul 27;14(14):R546-51. [2] Roth SY, et al. Annu Rev Biochem. 2001:70:81-120.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%