Acetyl-Histone H2B (Lys5) Rabbit mAb

Catalog No.: F1271

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Biological Description

Specificity Acetyl-Histone H2B (Lys5) Rabbit mAb recognizes endogenous levels of histone H2B only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones.
Background Histone H2B is one of the five main histone proteins, alongside H1/H5, H2A, H3, and H4. Within the nucleosome—the fundamental unit of chromatin—two molecules each of H2A, H2B, H3, and H4 assemble into an octameric core around which DNA wraps in a left-handed supercoil. Histones are subject to a range of post-translational modifications (PTMs) that play vital roles in regulating transcription, DNA replication and repair, and maintaining chromosomal integrity. During transcriptional activation, the histone acetyltransferases p300 and CBP catalyze the acetylation of multiple lysine residues on the N-terminal tail of histone H2B, particularly at Lys5, Lys12, Lys15, and Lys20. This acetylation reduces the positive charge on histone tails, thereby weakening their interactions with negatively charged DNA and between adjacent nucleosomes. As a result, chromatin becomes less compact and more accessible to transcription factors and other DNA-binding proteins. Furthermore, acetylation of specific lysine residues generates recognition sites for transcriptional co-regulators and chromatin remodeling factors that possess bromodomains—structural motifs that specifically bind to acetylated lysines—facilitating their recruitment to target gene promoters and enhancing gene expression.

Usage Information

Application WB, IP, IHC, IF, ChIP Dilution
WB IP IHC IF CHIP
1:1000 1:100 1:1000 1:400 1:50
Reactivity Human, Mouse, Rat, Monkey
Source Rabbit MW 14 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data