Molecular Weight(MW): 258.23
Thalidomide was introduced as a sedative drug, immunomodulatory agent and also is investigated for treating symptoms of many cancers. Thalidomide inhibits an E3 ubiquitin ligase, which is a CRBN-DDB1-Cul4A complex.
Cited by 12 Publications
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(E) MM.1S cells were treated with Thal(Thalidomide) or Pom for 48 hours. In each case, western blot was carried out using cell lysates and the indicated Abs.
Blood, 2017, 129(10):1308-1319. Thalidomide purchased from Selleck.
Thalidomide and lenalidomide increase the protein level of WT CRBN. Plasmids expressing FS-CRBN WT were transfected into HEK293T cells and then cells were equally divided into each well of a 6-well plate. The cells were treated with thalidomide (100 µM) or lenalidomide (10 µM) for indicated time.
FASEB Journal, 2015, fj.15-274050. Thalidomide purchased from Selleck.
PC cells were treated with 100 μM thalidomide for 24 h, and EMT markers were detected (n=3/group)
Oncotarget, 2016, 7(25):38539-38550. Thalidomide purchased from Selleck.
Murine bone marrow-derived DC (cultured for 4 d) conferred thalidomide (30 μM), PYR-41 (5 μM), or DMSO treatment prior to ovalbumin (50 μg/ml) or PBS pulse. The effects of PYR-41 and thalidomide on cross-presentation were determined by confocal microscope (b). For immunofluorescence observations, cross-presented OVA was stained with red (25-D1.16), EEA1 and Rab7 are all stained with green, and nuclei were counterstained with blue (DAPI). Original magnification, ×600.
J Immunol Res, 2018, 2018:5070573. Thalidomide purchased from Selleck.
Purity & Quality Control
Choose Selective E3 Ligase Inhibitors
|Description||Thalidomide was introduced as a sedative drug, immunomodulatory agent and also is investigated for treating symptoms of many cancers. Thalidomide inhibits an E3 ubiquitin ligase, which is a CRBN-DDB1-Cul4A complex.|
Thalidomide must be metabolized by the liver to form an epoxide that may be the active teratogenic metabolite.  Thalidomide selectively inhibits the production of human monocyte tumor necrosis factor alpha (TNF-alpha) when human monocytes are triggered with lipopolysaccharide and other agonists in culture.  Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation.  Thalidomide acts directly, by inducing apoptosis or G1 growth arrest, in MM cell lines and in patient MM cells that are resistant to melphalan, doxorubicin, and dexamethasone (Dex). Thalidomide enhances the anti-MM activity of Dex and, conversely, are inhibited by interleukin 6.  Thalidomide is a potent costimulator of primary human T cells in vitro, synergizing with stimulation via the T cell receptor complex to increase interleukin 2-mediated T cell proliferation and interferon gamma production. Thalidomide also increases the primary CD8+ cytotoxic T cell response induced by allogeneic dendritic cells in the absence of CD4+ T cells. 
|In vivo||Thalidomide (200 mg/kg) results in an inhibition of the area of vascularized cornea of rabbits that ranged from 30% to 51% in three experiments with a median inhibition of 36%. |
-  D'Amato RJ, et al. Proc Natl Acad Sci U S A, 1994, 91(9), 4082-4085.
-  Sampaio EP, et al. J Exp Med, 1991, 173(3), 699-703.
-  Moreira AL, et al. J Exp Med, 1993, 177(6), 1675-1680.
|In vitro||DMSO||51 mg/mL (197.49 mM)|
|Ethanol||2 mg/mL (7.74 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+1% Tween 80+ddH2O (suspension)
For best results, use promptly after mixing.
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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT02956538||Unknown status||Drug: Thalidomide|Drug: placebo(for thalidomide)||Crohn Disease||Sixth Affiliated Hospital Sun Yat-sen University||October 2016||Early Phase 1|
|NCT02966522||Unknown status||Drug: Thalidomide|Drug: Dexamethasone||Cardiac Amyloidosis||Seoul National University Hospital|CW pharmaceutical company||October 2016||Phase 2|
|NCT02778893||Recruiting||Drug: Conmana|Drug: Thalidomide||NSCLC||Henan Provincial Hospital||March 2016||Phase 4|
|NCT01937442||Completed||Drug: Thalidomide Celgene™||Multiple Myeloma||Celgene Corporation||November 2013||Phase 1|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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