Home Microbiology Antibiotics for Mammalian Cell Culture inhibitor - Fungal inhibitor Nystatin

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Nystatin Antibiotics for Mammalian Cell Culture inhibitor

Nystatin(Fungicidin), which belongs to the polyene group of antimycotics, is frequently used as a topical agent in the treatment of oro-pharyngeal candidosis.

Nystatin Antibiotics for Mammalian Cell Culture inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 926.09

Purity & Quality Control

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Mechanism of Action

In vitro
In vitro

Nystatin results in a significant reduction in buccal epithelial cell adhesion of all six Candida species. [1] This compound is an antibiotic that increases the permeability of plasma membranes to small monovalent ions, including chloridion. It increases apical chloridion permeability to the point where transepithelial chloridion transport is limited by transport across the basolateral membrane of tracheal epithelial cells, which reflects primarily the activity of the cotransporter. This chemical (400 units/ml) increases the basal level of transepithelial 36Cl flux approximately 1.5-fold and eliminates UTP stimulation of this flux. This treatment also abolishes UTP stimulation of saturable, basolateral [3H]bumetanide binding, a measure of functioning Na-K-Cl cotransporters in these cells; isoproterenol stimulation of binding is only mildly inhibited by nystatin treatment. [2] It significantly enhances endostatin uptake by endothelial cells through switching endostatin internalization predominantly to the clathrin-mediated pathway. This compound-enhanced internalization of endostatin also increases its inhibitory effects on endothelial cell tube formation and migration. [3]

In Vivo
In vivo

Nystatin combined with endostatin selectively enhances endostatin uptake and biodistribution in tumor blood vessels and tumor tissues but not in normal tissues of tumor-bearing mice, ultimately resulting in elevated antiangiogenic and antitumor efficacies of endostatin in vivo. [3] Liposomal this compound, at doses as low as 2 mg/kg of body weight/day, protects neutropenic mice against Aspergillus-induced death in a statistically significant manner at the 50-day time point compared to either the no-treatment, the saline, or the empty-liposome group. [4]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01531192 Completed
Very Low Birth Weight Infants
Zekai Tahir Burak Women''s Health Research and Education Hospital
 June 2012 Phase 4
NCT01411748 Unknown status
Anticandidal Property of Saccharomyces Boulardii on Very Low Birth Weight Infants
Zekai Tahir Burak Women''s Health Research and Education Hospital
 July 2011 Phase 4
NCT01427738 Completed
HIV-1 Infection
Advancing Clinical Therapeutics Globally for HIV/AIDS and Other Infections|National Institute of Allergy and Infectious Diseases (NIAID)
 June 2011 Phase 3
NCT03390374 Completed
Fungal Infections Systemic
Dr Cipto Mangunkusumo General Hospital
 October 2010 Phase 4
NCT02642900 Completed
Photochemotherapy Reaction
University of Sao Paulo
 July 2009 Phase 4

References
  • https://pubmed.ncbi.nlm.nih.gov/10895691/
  • https://pubmed.ncbi.nlm.nih.gov/8203506/
  • https://pubmed.ncbi.nlm.nih.gov/21482707/
  • https://pubmed.ncbi.nlm.nih.gov/9333054/

Chemical Information

Molecular Weight 926.09 Formula

C47H75NO17
CAS No. 1400-61-9 SDF Download SDF
Synonyms Fungicidin
Smiles CC1C=CC=CCCC=CC=CC=CC=CC(CC2C(C(CC(O2)(CC(C(CCC(CC(CC(CC(=O)OC(C(C1OC3CC(C(C(O3)C)O)O)C)C)O)O)O)O)O)O)O)C(=O)O)OC4C(C(C(C(O4)C)O)N)O

Storage and Stability

Storage (From the date of receipt)

In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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