Nevirapine (NSC 641530)
For research use only.
CAS No. 129618-40-2
Nevirapine (NSC 641530) is a non-nucleoside reverse transcriptase inhibitor (NNRTI) used to treat HIV-1 infection and AIDS.
Selleck's Nevirapine (NSC 641530) has been cited by 5 publications
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Cells were inoculated with 0.05 ng mock-exposed and semen-exposed HIV, and 0.5 ng HIV as infectivity-matched control. Infection rates were measured 3 d post infection by measuring β-galactosidase. Left: the mean enzyme activities. Middle: normalized infection rates in which reporter enzyme activities obtained from infected cells in the absence of nevirapine were set at 100%. Right: IC50 values.
Sci Transl Med, 2014, 6(262): 262ra157 . Nevirapine (NSC 641530) purchased from Selleck.
Purity & Quality Control
Choose Selective Reverse Transcriptase Inhibitors
|Description||Nevirapine (NSC 641530) is a non-nucleoside reverse transcriptase inhibitor (NNRTI) used to treat HIV-1 infection and AIDS.|
Nevirapine (NVP) itself is an inhibitor of only CYP3A4 at concentrations that are well above those of therapeutic relevance (Ki = 270 mM).  Nevirapine is a non-nucleoside RT inhibitor with a well-characterized inhibitory activity on RT enzymes of retroviral origin. Nevirapine is also an effective inhibitor of the endogenous RT in murine and human cell lines. Nevirapine exposure rescues the differentiation block present in acute myeloid leukemia (AML) cell lines and primary blasts from two AML patients, as indicated by morphological, functional and immunophenotypic assays.  Nevirapine, a dipyridodiazepinone, is a highly specific inhibitor of HIV-1 reverse transcriptase (RT) which exhibits an IC50 = 84 nM in enzyme assays and IC50 = 40nM against HIV-1 replication in cell culture.  Nevirapine alters the cleavage specificity of the RNase H, resulting Nevirapine-induced stimulation of RNase H activity beyond the increase expected from the change in cleavage specificity. 
|In vivo||4-CANVP is a major metabolite in all the male animals and the female mouse, dog, and monkey. 3-OHNVP is a major fecal metabolite in all animals except for the male rat. 4-CANVP is a major metabolite along with 12-OHNVP glucuronide in the bile of rats. |
-  Erickson DA, et al. Drug Metab Dispos, 1999, 27(12), 1488-1495.
-  Mangiacasale R, et al. Oncogene, 2003, 22(18), 2750-2761.
-  Grob PM, et al. AIDS Res Hum Retroviruses, 1992, 8(2), 145-152.
|In vitro||DMSO||53 mg/mL (199.02 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT02738502||Completed||Drug: darunavir monotherapy||Maternal-fetal Infection Transmission||ANRS Emerging Infectious Diseases|Institut National de la Santé Et de la Recherche Médicale France||July 6 2016||Phase 2|
|NCT02447159||Completed||Behavioral: Conditional Cash Transfers||HIV|HIV Infections|Pregnancy|Infant Newborn Diseases||University of California San Francisco|University of California Berkeley|New Incentives|Bill and Melinda Gates Foundation||August 2015||Not Applicable|
|NCT02429791||Active not recruiting||Drug: DTG 50 mg|Drug: RPV 25 mg|Drug: CAR||HIV Infections||ViiV Healthcare|Janssen Pharmaceuticals|GlaxoSmithKline||April 14 2015||Phase 3|
|NCT02422797||Active not recruiting||Drug: DTG 50 mg|Drug: RPV 25 mg|Drug: CAR||HIV Infections||ViiV Healthcare|Janssen Pharmaceuticals|GlaxoSmithKline||April 21 2015||Phase 3|
|NCT03023033||Completed||Behavioral: mHealth messaging|Behavioral: Transport Payments||HIV|AIDS||Elizabeth Glaser Pediatric AIDS Foundation|Population Council|Ministry of Health Tanzania||October 2014||Not Applicable|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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