Lamivudine (BCH-189)

Catalog No.S1706 Synonyms: GR109714X, BCH-189

For research use only.

Lamivudine (BCH-189, GR109714X) is a potent nucleoside analog reverse transcriptase inhibitor, used for treatment of chronic HBV and HIV/AIDS. It works by blocking the HIV reverse transcriptase and hepatitis B virus polymerase.

Lamivudine (BCH-189) Chemical Structure

CAS No. 134678-17-4

Selleck's Lamivudine (BCH-189) has been cited by 11 Publications

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Biological Activity

Description Lamivudine (BCH-189, GR109714X) is a potent nucleoside analog reverse transcriptase inhibitor, used for treatment of chronic HBV and HIV/AIDS. It works by blocking the HIV reverse transcriptase and hepatitis B virus polymerase.
Targets
Reverse transcriptase [1]
In vitro

Lamivudine’s anti- HBV activity, like its anti-HIV activity, has been shown to depend on the ability of LMV-TP to serve as both substrate and inhibitor of the DNA- and RNA-dependent polymerase activities of the HBV P gene product. Lamivudine owes its activity to the remarkably broad substrate specificity of deoxycytidine kinase and the unusual substrate preference of the HBV polymerases for dNTPs with the unnatural L-conformation, whereas the anti-HBV activity of PCV appears to depend on several factors including optimal phosphorylation (sufficient for antiviral activity but not cytotoxicity) by key cellular enzymes, the long intracellular half-life of PCV-TP and the ability of PCV-TP to inhibit the HBV RT priming reaction as well as RT and DNA polymerase activity. [1] Lamivudine and Penciclovir inhibits duck hepatitis B virus (DHBV) replication to a comparable extent when used alone, and in combination, the two nucleoside analogs acts synergistically over a wide range of clinically relevant concentrations. Lamivudine combined with Penciclovir is more effective in reducing the normally recalcitrant viral covalently closed circular (CCC) DNA form of DHBV than either drug alone. [2] Lamivudine inhibits p24 antigen production by HIV-I in PBMC, with ED50s ranging from 0.07 μM to 0.2 μM. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MT-4 MYTGeY5kfGmxbjDhd5NigQ>? NVznU4xPS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJN6dnSrY3Gg[o9zdWG2aX;uJIJ6KDVyJTDpckBJUVZvMTDpcoZm[3SnZDDNWE01KGOnbHzzMEBKSzVyPUCuNe69VQ>? M2nMbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzhyM{W0NlkoRjhyM{W0Nlk9N2F-
PBMC M3v5U2Z2dmO2aX;uJIF{e2G7 NF;vfHJEd26lZX70doF1cW:wIILldZVqemWmIH\vdkBidnSrdnnyZYwh[WO2aY\peJkhcW5iUFLNR{ApcHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczmgbY5n\WO2ZXSge4l1cCCKSW[tNUB{fHKjaX6gUGFXNCCHQ{WwQVAvODBzON88US=> NH\US|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NEGwPVc2Lz56NEGwPVc2RC:jPh?=
PBMC M3;VXWZ2dmO2aX;uJIF{e2G7 M4nNTmNwdmOnboTyZZRqd25icnXxeYlz\WRiZn;yJIFvfGm4aYLhcEBi[3Srdnn0fUBqdiCSQl3DJEhpfW2jbjDw[ZJqeGincnHsJIJtd2:mIH3vco9vfWOuZXHyJINmdGy|KTDpcoZm[3SnZDD3bZRpKEiLVj2xJJN1emGrbjDMRXYtKEWFNUC9NE4zOc7:TR?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PDFyOUe1K|45PDFyOUe1QE9iRg>?
PBMC Ml\HRY51cS2KSW[tNUBie3OjeR?= NVj4NIF{SW62aT3ITXYuOSCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJJBmemmyaHXyZYwh[myxb3SgcY9vd263Y3zlZZIhMFCETTmgZ4VtdHNiaX7m[YN1\WRid3n0bEBJUVZvMTDzeJJicW5iTFHWMEBGSzVyPUCuNFAyQM7:TR?= M{L4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh2MkO1PVEoRjh2MkO1PVE9N2F-
PBMC M3;jcGFvfGlvSFnWMVEh[XO|YYm= M1zOU2FvfGlvSFnWMVEh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDw[ZJqeGincnHsJIJtd2:mIH3vco9vfWOuZXHyJEhRSk1rIHPlcIx{KGmwZnXjeIVlKHerdHigTGlXNTFic4TyZYlvKEyDVjygSWM2OD1yLkC2{txO M1PqPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh2MkO1PVEoRjh2MkO1PVE9N2F-
PBMC MYjBcpRqNUiLVj2xJIF{e2G7 M1X3eWFvfGlvSFnWMVEh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDw[ZJqeGincnHsJIJtd2:mIH3vco9vfWOuZXHyJEhRSk1rIHPlcIx{KGmwZnXjeIVlKHerdHigTGlXNTFic4TyZYlvKEyDVjygSWM2OD1yLkKx{txO MkL6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDR{M{W5NUc,QDR{M{W5NVww[T5?
PBMC MX7BcpRqNUiLVj2xJIF{e2G7 MnvORY51cS2KSW[tNUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIIDldolxcGW{YXygZoxwd2RibX;uc452[2ynYYKgLHBDVSliY3XscJMhcW6oZXP0[YQhf2m2aDDITXYuOSC|dILhbY4hVEGYLDDFR|UxRTFyLkJOwG0> M{W5R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh2MkO1PVEoRjh2MkO1PVE9N2F-
2.2.15 MkG5SpVv[3Srb36gZZN{[Xl? MUTJckB3cXS{bzDl[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGincHH0bZRqeyCEII\pdpV{KHejczDk[ZRmem2rbnXkJIlvKDJwMj6xOUBk\WyuczygSWM2OD1yLkCwPO69VQ>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86OzBzNkW5K|46OzBzNkW5QE9iRg>?
PBMC NIDzVGxCdnSrLVjJWk0yKGG|c3H5 NEf5Ro9KdiC4aYTyc{BidnSrLVjJWkBi[3Srdnn0fUBqdiCyZYLpdIhmemGuIHLsc49lKG2xbn;ueYNt\WG{IHPlcIx{NCCHQ{WwQVAvODgQvF2= MmLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTNyMU[1PUc,QTNyMU[1PVww[T5?
2.2.15 NX60N29vSW62aY\pdoFtKGG|c3H5 MlvLRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTIVx[XSrdHnzJGIhfmm{dYOgLGhDXiliaX6gNk4zNjF3IHPlcIx{NCCHQ{WwQVAvODB6zszN M2PM[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMEWyO|k2Lz5zMUC1Nlc6PTxxYU6=
PBMC NIfCOGFCdnSrdnnyZYwh[XO|YYm= M1PpSGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGludXWwb3Tl[olkcWWwY4mgeolzfXNidInw[U0yKCiKSW[tNUkhcW5icHXybZBp\XKjbDDicI9w\CCvb37vcpVkdGWjcjDj[YxteyxiRVO1NF0xNjB5zszN NV\zN5kxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGwOVI4QTVpPkGxNFUzPzl3PD;hQi=>
H9 NHe4bFJCdnSrdnnyZYwh[XO|YYm= M1:zNmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY4hcHWvYX6gTFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGK7IH\vdo1igmGwLXLhd4VlKGOxbo\lcpRqd26jbDDjc4xwemmvZYTybYMhfGWlaH7pdZVmNCCHQ{WwQVAvODcQvF2= NFXTSHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUSzNFAyQSd-MUG0N|AxOTl:L3G+
H9 MmL5RY51cX[rcnHsJIF{e2G7 NEPUO41CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIGLGJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCkeTDmc5Ju[Xqjbj3iZZNm\CClb372[Y51cW:wYXygZ49td3KrbXX0dolkKHSnY3jubZF2\SxiRVO1NF0xNjB4zszN NH;x[5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUSzNFAyQSd-MUG0N|AxOTl:L3G+
PBMC NGf6XmJCdnSrdnnyZYwh[XO|YYm= Mn\JRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXNTG6eFLSWUBufXSjboSgd5Rz[WmwIHnuJIh2dWGwIGDCUUBk\WyuczygSWM6OD1yLkC4{txO M{S1VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{izNFE1Lz5zMkO4N|AyPDxxYU6=
PBMC M1;UOGZ2dmO2aX;uJIF{e2G7 M3SybmFkfGm4aYT5JIFo[Wmwc4SgUIFucX[3ZHnu[U1z\XOrc4ThcpQhfmm{dYOgLGhKXi1zWGjCVnUqKGmwIHj1cYFvKFCETTDj[YxteyxiZYjwdoV{e2WmIHHzJGVEPTBuIFXDOVA:OC5yM{ZOwG0> MkL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6NUK3OVUoRjF{OEWyO|U2RC:jPh?=
PBMC M3KyV2Z2dmO2aX;uJIF{e2G7 M1nLTmlvKH[rdILvJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDD0bIUhUEmYLUGgUGEyKHKncHzpZ4F1cW:wIH;uJHBDVUO|IHPlcIwhMHCncnnwbIVz[WxiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMqNCCHQ{WwQVAvODIQvF2= NIfqbmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMki1Nlk1Oyd-MUK4OVI6PDN:L3G+
PBMC NHL1eXdHfW6ldHnvckBie3OjeR?= NHv5UndG\m[nY4TpeoUh[2:wY3XueJJifGmxbjD0c{BqdmirYnn0JGhKXi1zIFzBTUBkgXSxcHH0bIlkcXS7IHnuJHBDVUNiY3XscJMhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:sJGVEPTB;MD6wNVE3|ryP NFnSclE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEm3NVg6QCd-MUS5O|E5QTh:L3G+
HepG2.2.15 M{\Sd2Z2dmO2aX;uJIF{e2G7 NFjxV4hG\m[nY4TpeoUh[2:wY3XueJJifGmxbjD0c{BqdmirYnn0JIhmeGG2aYTpd{BDKH[rcoXzJIN6fG:yYYTobYNqfHliaX6gTIVxTzJwMj6xOUBk\WyuczD3ZZMh\GW2ZYLtbY5m\CCrbjD2bZRzdyxiRVO1NF0xNjMQvF2= MnHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR7N{G4PVgoRjF2OUexPFk5RC:jPh?=
MT-4 M{P4NWZ2dmO2aX;uJIF{e2G7 MUHF[oZm[3SrdnWgZ49v[2WwdILheIlwdiC2bzDpcohq[mm2IFjJWk0yKEmLSVKgZ5l1d3CjdHjpZ4l1gSCrbjDNWE01KGOnbHzzJJdieyCmZYTldo1qdmWmIHnuJJZqfHKxLDDFR|UxRTBwNd88US=> M1LKNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2OUexPFk5Lz5zNEm3NVg6QDxxYU6=
PBMC MlXwRY51cX[rcnHsJIF{e2G7 Mn3kRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXiC4aYL1d{h5gEKUVTmgbY4hUHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDtc45wdnWlbHXhdkApWEKPKTDj[YxteyxiRVO5NF0xNjB6zszN NWPneFRRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWwNlc5PTRpPkG1NFI4QDV2PD;hQi=>
PBMC M1XWe2Z2dmO2aX;uJIF{e2G7 MmT6SYZn\WO2aY\lJINwdmOnboTyZZRqd25iYXfhbY5{fCCZaXzkJHR6eGViSFnWMVEhfmm{dYOgbY4hcHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczygSWM2OD1yLkC0Ne69VQ>? NGPIVFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUG4PVA{Pid-MUWxPFkxOzZ:L3G+
CEM-SS M1XTTGFvfGm4aYLhcEBie3OjeR?= MmX5OkBl[Xm| MYHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnu[oVkfGWmIHnuJIh2dWGwIFPFUU1UWyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYX\0[ZIhPiCmYYnzJIJ6KE2WUzDhd5NigSxiRVS1NF0xNjN4zszN MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJzN{K4NUc,OTV{MUeyPFE9N2F-
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2.2.15 MmjMSpVv[3Srb36gZZN{[Xl? NF;3fVVKdmirYnn0bY9vKG:oIIP5cpRp\XOrczDv[kBmgHS{YXPlcIx2dGG{II\pdolwdiCGTlGgdoVt\WG|ZTDpckBkfWy2dYLl[EBpfW2jbjDo[ZBifG:kbHHzeI9u[SB{LkKuNVUh[2WubIOsJGVEPTB;MD6wOFjPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzM{exN{c,OTZ{MUO3NVM9N2F-
2.2.15 NVLOTlVDTnWwY4Tpc44h[XO|YYm= MVvJcohq[mm2aX;uJI9nKHO7boTo[ZNqeyCxZjDlfJRz[WOnbHz1cIFzKH[rcnnvckBFVkFicnXs[YF{\SCrbjDjeYx1fXKnZDDoeY1idiCqZYDheI9jdGG|dH;tZUAzNjJwMUWgZ4VtdHNuIFXDPVA:OC5zNUJOwG0> NGHWemE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxN|cyOyd-MU[yNVM4OTN:L3G+
2.2.15 NHX1S5FHfW6ldHnvckBie3OjeR?= NGOxfYdKdmirYnn0bY9vKG:oIIP5cpRp\XOrczDv[kBqdnS{YXPlcIx2dGG{IFjCWkBFVkFicnXwcIlk[XSrdnWgbY51\XKvZXTpZZRmeyCrbjDjeYx1fXKnZDDoeY1idiCqZYDheI9jdGG|dH;tZUAzNjJwMUWgZ4VtdHNuIFXDOVA:OC5{NUJOwG0> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzM{exN{c,OTZ{MUO3NVM9N2F-
2.2.15 NV76V5hlTnWwY4Tpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKHO7boTo[ZNqeyCxZjDpcpRz[WOnbHz1cIFzKEiEVjDEUmEhemWybHnjZZRqfmViaX70[ZJu\WSrYYTld{BqdiCldXz0eZJm\CCqdX3hckBp\XCjdH;icIF{fG:vYTCyMlIvOTViY3XscJMtKEWFOUC9NE43PDkQvF2= NE\3VXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxN|cyOyd-MU[yNVM4OTN:L3G+
2.2.15 MUTGeY5kfGmxbjDhd5NigQ>? MV;Jcohq[mm2aX;uJI9nKHerbHSgeJlx\SCKQm[geJJidnOoZXP0[YQhcW5iaIXtZY4hcGWyYYTvZoxie3SxbXGgNk4zNjF3IHPlcIx{NCCHQ{WwQVIvOc7:TR?= M{WxblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUO5N|k{Lz5zNkWzPVM6OzxxYU6=
hepatoma 2.2.15 M3LVVWFvfGm4aYLhcEBie3OjeR?= NIfVV3FCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhUEKYIHnuJIh2dWGwIHjldIF1d22jIEKuNk4yPSClZXzsd{whTUN3ME2yMlHPxE1? MkSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5NUmxNVIoRjF4N{W5NVEzRC:jPh?=
2.2.15 NGDsUZpHfW6ldHnvckBie3OjeR?= NYLDXphVUW6qaXLpeIlwdiCxZjDIRnYhemWybHnjZZRqd25iaX6gNk4zNjF3IHPlcIx{KGK7IFTORUBpgWK{aXTpfoF1cW:wIHHzd4F6NCCHQ{WwQVAvODMQvF2= NYTqfFNMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OjZpPkG3NFA1PzJ4PD;hQi=>
MDCK2 NFfkcVVHfW6ldHnvckBie3OjeR?= NYjzc5loOTBidV2= M1;hTmlvcGmkaYTpc44hd2ZiaIXtZY4hVVKSMjDlfJBz\XO|ZXSgbY4hVUSFS{KgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcW62cnHj[YxtfWyjcjDDUWYh\my3b4Lld4NmdmOnIHH0JFExKHWPIHL5JGNOTkSDIHHzd4F6 NX;QWG41RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexO|I{OTFpPkG3NVczOzFzPD;hQi=>
MDCK2 MV7GeY5kfGmxbjDhd5NigQ>? NGfHclYyOCC3TR?= NIHteGVKdmirYnn0bY9vKG:oIHj1cYFvKE2UUEOg[ZhxemW|c3XkJIlvKE2GQ1uyJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGmwdILhZ4VtdHWuYYKgR21HKG[udX;y[ZNk\W6lZTDheEAyOCC3TTDifUBEVU[GQTDhd5NigQ>? MnXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzN{KzNVEoRjF5MUeyN|EyRC:jPh?=
MDCK2 MoL6SpVv[3Srb36gZZN{[Xl? MUOxNEB2VQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiZYjwdoV{e2WmIHnuJG1FS0t{IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIlvfHKjY3XscJVt[XJiQ13GJIZtfW:{ZYPj[Y5k\SCjdDCxNEB2VSCkeTDDUWZFSSCjc4PhfS=> NETLOZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G3NlMyOSd-MUexO|I{OTF:L3G+
Huh7 M3flO2Z2dmO2aX;uJIF{e2G7 M3X0WWlvcGmkaYTpc44hd2Zid3ns[EB1gXCnIFjCWkBz\XCuaXPheIlwdiCrbjDIeYg4KGOnbHzzMEBGSzVyPUJOwG0> NVPMeXMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OjdpPkG3N|cyQDJ5PD;hQi=>
PBMC MlPRRY51cX[rcnHsJIF{e2G7 NYHCfJdrSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDMRWkhcW5iaIXtZY4hWEKPIHPlcIx{NCCHQ{WwQVAvODJ4zszN NXPhTVk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NFM6QTZpPkG3OFA{QTl4PD;hQi=>
PBMC M1K0S2FvfGm4aYLhcEBie3OjeR?= NGrENHVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIFzBTUBqdiCqdX3hckBRSk1iY3XscJMtKEWFOUC9NE4yOs7:TR?= NHHoNZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SwN|k6Pid-MUe0NFM6QTZ:L3G+
HepG2.2.15 MWfBcpRqfmm{YXygZZN{[Xl? MkniRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGmwIFjldGczNjJwMUWgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h\Xi2cnHj[YxtfWyjcjD2bZJidCCGTlGgcIV3\WxiYomgVnQuWEOULDDFR|UxRTBwMd88US=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyNECwOkc,OTd2MESwNFY9N2F-
Huh7 Mn\qRY51cX[rcnHsJIF{e2G7 NFrZRZo2KGSjeYO= M{HXTmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCpZX7veJlx\SCGIIfpeIghemW4ZYLz[UB1emGwc3PybZB1[XOnIFy4NGkhdXW2YYTpc44hcW5iaIXtZY4hUHWqNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFUh\GG7czygSWM2OD1yLkCz{txO NFnpXIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SzPFA1Pyd-MUe0N|gxPDd:L3G+
Huh7 M3\hUWFvfGm4aYLhcEBie3OjeR?= NGLLdHo2KGSjeYO= NEH4[ZZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSmWDZz3k[YZq[2mnboSgTGJXKGenbn;0fZBmKERid3n0bEBxemWlb4LlJGcyQDl4QTDteZRifGmxbjDhcoQhemW4ZYLz[UB1emGwc3PybZB1[XOnIFy4NGkhdXW2YYTpc44hcW5iaIXtZY4hUHWqNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFUh\GG7czygSWM2OD1yLkCz{txO NEnUZY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SzPFA1Pyd-MUe0N|gxPDd:L3G+
Huh7 NY\zcm8zSW62aY\pdoFtKGG|c3H5 MX:1JIRigXN? M3nP[mFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCpZX7veJlx\SCGIHnuJIh2dWGwIFj1bFch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB3IHThfZMtKEWFNUC9NE41Oc7:TR?= MkKwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2M{iwOFcoRjF5NEO4NFQ4RC:jPh?=
Huh7 MWrBcpRqfmm{YXygZZN{[Xl? MnvROUBl[Xm| NUH2[GJoSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKnQXet[IVncWOrZX70JGhDXiCpZX7veJlx\SCGIIfpeIgheHKnY3;y[UBIOTh7NlGgcZV1[XSrb36gbY4hcHWvYX6gTJVpPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEWg[IF6eyxiRVO1NF0xNjR4zszN MkDlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2M{iwOFcoRjF5NEO4NFQ4RC:jPh?=
HepG2(2.2.15) M{TkZWFvfGm4aYLhcEBie3OjeR?= M4HrPGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbjDhfZchf2muZDD0fZBmKEincFeyLFIvOi5zNTmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBFVkFicnXwcIlk[XSrb36sJGlEPTB;MD6wOe69VQ>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR6OEixO{c,OTd2OEi4NVc9N2F-
HepG2(2.2.15) NY\6cWR6TnWwY4Tpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKEiEVjDwdo9ud3SncjDpckBpfW2jbjDI[ZBIOih{LkKuNVUqKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkC4{txO M4rofFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEi4PFE4Lz5zN{S4PFgyPzxxYU6=
HepW10 M1HrXWFvfGm4aYLhcEBie3OjeR?= MmTORY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGmwIHHkdolidXmlaX6tdoV{cXO2YX70JJdqdGRidInw[UBJ\XCZMUCgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBFVkFicnXwcIlk[XSrb36sJGlEPTB;MD6x{txO M37rb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEi4PFE4Lz5zN{S4PFgyPzxxYU6=
HBV-Met MYHBcpRqfmm{YXygZZN{[Xl? MV7BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYhcW5ibX;1d4Uh[Xm5IIfpcIQhfHmyZTDIRnYuVWW2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygSG5CKHKncHzpZ4F1cW:wLDDJR|UxRTBwMt88US=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR6OEixO{c,OTd2OEi4NVc9N2F-
HepG2.2.15 MXzGeY5kfGmxbjDhd5NigQ>? NFTFWo05KGSjeYO= MmnETY5pcWKrdHnvckBw\iCleYTvdIxie22rYzDIRnYhTE6DIIP5cpRp\XOrczDpckBJ\XCJMj6yMlE2KGOnbHzzJIFnfGW{IEig[IF6eyxiSVO1NF0xNjN6zszN MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR7OUi4PUc,OTd2OUm4PFk9N2F-
PBMC NEfBWZNHfW6ldHnvckBie3OjeR?= NVezd|NYOzByIH3n M3zmNFch\GG7cx?= M4Dl[mNu[XhiaX6gTGlXNWmwZnXjeIVlKGi3bXHuJIF{e2W|c3XkJIF{KEyjbXn2eYRqdmVibX;uc5Bpd3OyaHH0[UBt\X[nbDDpckBRSk2FIHH0JFMxOCCvZzDRSEBnd3JiNzDkZZl{KG2nYYP1doVlKHCncjCxNEc3KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIIrp[I93fWSrbnWsJGNu[Xh;MD6wNFAxODB6zszN M2\UT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[0N|I5Lz5zN{[2OFMzQDxxYU6=
PBMC NUfwWZQ{TnWwY4Tpc44h[XO|YYm= MkHCN|AxKG2p NWjOOWl4PyCmYYnz MVXDcYF5KGmwIFjJWk1qdm[nY4Tl[EBpfW2jbjDhd5Nme3OnZDDhd{BN[W2rdoXkbY5mKGSrcHjvd5Bp[XSnIHzleoVtKGmwIGDCUWMh[XRiM{CwJI1oKFGGIH\vdkA4KGSjeYOgcYVie3W{ZXSgdIVzKDFyJ{[gZ4VtdHNiaX6gdJJme2WwY3Wgc4Yhgmmmb4\1[Ilv\SxiQ33hfF0xNjByMECwOFTPxE1? M2fqSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[0N|I5Lz5zN{[2OFMzQDxxYU6=
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MAGIC-5A NY\OV4VMSW62aY\pdoFtKGG|c3H5 NE\J[oE1OCCqcoO= M{PxNWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIF7MOE0{KHC{b3T1Z4VlKG[{b32g[pVtdCCuZX7neIgheFJ7ZHXseIFCeGFiaX7m[YN1\WRiaX6gbJVu[W5iTVHHTWMuPUFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBN[WNtIH\vZ4kh\XiycnXzd4lwdiCjZoTldkA1OCCqcoOsJGVEPTB;MD64PO69VQ>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl4N{mxN{c,OTd7Nke5NVM9N2F-
MAGIC-5A NXvPSYh5SW62aY\pdoFtKGG|c3H5 MVW0NEBpenN? MYnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGlXOiCUT1SgdJJw\HWlZXSg[pJwdSCodXzsJIxmdme2aDDwVm9FQSCrbn\lZ5Rm\CCrbjDoeY1idiCPQVfJR{02SSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxi[ytiZn;jbUBmgHC{ZYPzbY9vKGGodHXyJFQxKGi{czygSWM2OD1{zszN NHXyVZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m2O|kyOyd-MUe5Olc6OTN:L3G+
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MT2 NFvORXlCdnSrdnnyZYwh[XO|YYm= MoPZOUBl[Xm| NFvyXJJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEAxNjB3IF3PTUB4cWymIIT5dIUhUEmYMTDOUFQuOyCrbn\lZ5Rm\CCrbjCyJIhzNXC{ZYTy[YF1\WRiaIXtZY4hVVR{IHPlcIx{KG2nYYP1doVlKGGodHXyJFUh\GG7czDifUBOXFNiYYPzZZktKEWFNUC9N{4zPDgQvF2= M1PublxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{G2OVIyLz5zOEOxOlUzOTxxYU6=
HepG2(2.2.15) M4DNRmFvfGm4aYLhcEBie3OjeR?= NGr4SXgzPCCqcoO= NILEenNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX6gbJVu[W5iSHXwS|IpOi5{LkG1LUBk\WyuczDhd5Nme3OnZDDhd{BmgHS{YXPlcIx2dGG{IFjCWkB3cXKrb36gSG5CKGyndnXsJIFnfGW{IEK0JIhzeyxiRVO1NF0xNjB2ON88US=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB5Mk[5OEc,OTlyN{K2PVQ9N2F-
HepG2(2.2.15) Mn\PRY51cX[rcnHsJIF{e2G7 MkLONlQhcHK| NX;hSnY{SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHnuJIh2dWGwIFjldGczMDJwMj6xOUkh[2WubIOgZZN{\XO|ZXSgZZMhemWybHnjZZRqd25iaX70[ZJu\WSrYYTld{Bi\nSncjCyOEBpenNuIFXDOVA:OC5zNd88US=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB5Mk[5OEc,OTlyN{K2PVQ9N2F-
Huh7 MnvXRY51cX[rcnHsJIF{e2G7 NEDOcYk1KGSjeYO= NFvhWJhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBt[W2rdoXkbY5mNXKnc3nzeIFvfCC5aXzkJJR6eGViSFLWJIlvKGi3bXHuJGh2cDdiY3XscJMh[W[2ZYKgOEBl[Xm|IHL5JIlvfHKjY3XscJVt[XJiSFLWJGRPSSC{ZYDsbYNifGmxbjDhd5NigSxiRVO1NF0xNjMQvF2= NUPn[GZsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwO|I3QTRpPkG5NFczPjl2PD;hQi=>
Huh7 NUTiWWJySW62aY\pdoFtKGG|c3H5 MnPEOEBl[Xm| MmHRRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgcIFucX[3ZHnu[U1z\XOrc4ThcpQhUEKYIILleoVze2VidILhcpNkemmydHHz[UAzOz[WIH31eIFvfCCrbjDoeY1idiCKdXi3JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCrboTyZYNmdGy3bHHyJGhDXiCGTlGgdoVxdGmlYYTpc44h[XO|YYmsJGVEPTB;MD6z{txO MmDvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyN{K2PVQoRjF7MEeyOlk1RC:jPh?=
Huh7 MmPKRY51cX[rcnHsJIF{e2G7 NHvpRms1KGSjeYO= M4XTUWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIFl\W[xdnnyMZJme2m|dHHueEB4cWymIIT5dIUhUEKYIHnuJIh2dWGwIFj1bFch[2WubIOgZYZ1\XJiNDDkZZl{KGK7IHnueJJi[2WubIXsZZIhUEKYIFTORUBz\XCuaXPheIlwdiCjc4PhfUwhTUN3ME2xMlPPxE1? M1;Re|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
Huh7 M3:4UmFvfGm4aYLhcEBie3OjeR?= NX;mTIdjPCCmYYnz MlvERY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgZYRm\m:4aYKtdoV{cXO2YX70JGhDXiC{ZY\ldpNmKHS{YX7zZ5JqeHSjc3WgUVIxPFZibYX0ZY51KGmwIHj1cYFvKEi3aEegZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KGmwdILhZ4VtdHWuYYKgTGJXKESQQTDy[ZBtcWOjdHnvckBie3OjeTygSWM2OD1zLkZOwG0> MlnnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyN{K2PVQoRjF7MEeyOlk1RC:jPh?=
Huh7 MkfSRY51cX[rcnHsJIF{e2G7 MoW5OEBl[Xm| MmLYRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgZYRm\m:4aYKtdoV{cXO2YX70JGhDXiC{ZY\ldpNmKHS{YX7zZ5JqeHSjc3WgUFE5OE1ibYX0ZY51KGmwIHj1cYFvKEi3aEegZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KGmwdILhZ4VtdHWuYYKgTGJXKESQQTDy[ZBtcWOjdHnvckBie3OjeTygSWM2OD1zLkdOwG0> M{PmT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
Huh7 MmCwRY51cX[rcnHsJIF{e2G7 M2nxWlQh\GG7cx?= M1PIdmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIFl\W[xdnnyMZJme2m|dHHueEBJSlZicnX2[ZJ{\SC2cnHud4NzcXC2YYPlJGxON3KndnXyd4UhfHKjboPjdolxfGG|ZTDNWkBld3WkbHWgcZV1[W62IHnuJIh2dWGwIFj1bFch[2WubIOgZYZ1\XJiNDDkZZl{KGK7IHnueJJi[2WubIXsZZIhUEKYIFTORUBz\XCuaXPheIlwdiCjc4PhfUwhTUN3ME2xMlbPxE1? MkTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyN{K2PVQoRjF7MEeyOlk1RC:jPh?=
Huh7 NFz5dJBCdnSrdnnyZYwh[XO|YYm= M1HIRVQh\GG7cx?= MmHuRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgZYRm\m:4aYKtdoV{cXO2YX70JGhDXiC{ZY\ldpNmKHS{YX7zZ5JqeHSjc3WgUVIxPElibYX0ZY51KGmwIHj1cYFvKEi3aEegZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KGmwdILhZ4VtdHWuYYKgTGJXKESQQTDy[ZBtcWOjdHnvckBie3OjeTygSWM2OD1zLklOwG0> NXnlSHdPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwO|I3QTRpPkG5NFczPjl2PD;hQi=>
Huh7 NGTMelBCdnSrdnnyZYwh[XO|YYm= NYfVWWdRPCCmYYnz M1jWVmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIFl\W[xdnnyMZJme2m|dHHueEBJSlZicnX2[ZJ{\SC2cnHud4NzcXC2YYPlJFI{PlRibYX0ZY51KGmwIHj1cYFvKEi3aEegZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KGmwdILhZ4VtdHWuYYKgTGJXKESQQTDy[ZBtcWOjdHnvckBie3OjeTygSWM2OD15LkhOwG0> NF7OOII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC3NlY6PCd-MUmwO|I3QTR:L3G+
Huh7 NXLJV2h[SW62aY\pdoFtKGG|c3H5 M1\SXVQh\GG7cx?= MWPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDsZY1qfnWmaX7lMZJme2m|dHHueEBJSlZicnX2[ZJ{\SC2cnHud4NzcXC2YYPlJGwyQDCPIH31eIFvfCCrbjDoeY1idiCKdXi3JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCrboTyZYNmdGy3bHHyJGhDXiCGTlGgdoVxdGmlYYTpc44h[XO|YYmsJGVEPTB;MUFOwG0> M3\KXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
HuH7 NEm3PHlCdnSrdnnyZYwh[XO|YYm= NF72[FQzPCCqcoO= MXXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGJXKGmwZnXjeIVlKGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygeJJidnOrZX70JJRz[W6|ZnXjeIlwdiCjZoTldkAzPCCqcoOsJGVEPTB;MD6y{txO MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN7OE[0PEc,OTl|OUi2OFg9N2F-
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HuH7 NXHlOHhNSW62aY\pdoFtKGG|c3H5 NYTMZ2duOjRiaILz NF3TXI5CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaHHyZo9zcW6pIGLORUBxd2y7bXXyZZNmKE5{M{\UJI12fGGwdDDn[Y5mKGmwZnXjeIVlKGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygeJJidnOrZX70JJRz[W6|ZnXjeIlwdiCjZoTldkAzPCCqcoOsJGVEQTB;MT61{txO M4O2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{m4OlQ5Lz5zOUO5PFY1QDxxYU6=
HuH7 M37DZmFvfGm4aYLhcEBie3OjeR?= NICwdo0zPCCqcoO= NIX3[2lCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhUEKYIHnu[oVkfGWmIHnuJIh2dWGwIFj1TFch[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5idnnyZYwhfHKjboPp[Y51KHS{YX7z[oVkfGmxbjDh[pRmeiB{NDDodpMtKEWFOUC9N:69VQ>? NXfXWlVoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzPVg3PDhpPkG5N|k5PjR6PD;hQi=>
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HuH7 Ml70RY51cX[rcnHsJIF{e2G7 NY\0N25COjRiaILz MYfBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYhcGG{Yn;ybY5oKFKQQTDwc4x6dWW{YYPlJGwyQDCPIH31eIFvfCCpZX7lJIlv\mWldHXkJIlvKGi3bXHuJGh2UDdiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6geolz[WxidILhcpNq\W62IITyZY5{\mWldHnvckBi\nSncjCyOEBpenNuIFXDPVA:PDIQvF2= NFzrNo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO5PFY1QCd-MUmzPVg3PDh:L3G+
MT2 MmjiRY51cX[rcnHsJIF{e2G7 M3ewc2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiaX7m[YN1\WRiaX6gbJVu[W5iTWSyJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25uIFnDOVA:OC54zszN NWnvNIhZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1PVY5QDVpPkG5OVk3QDh3PD;hQi=>
HeLa P4/R5 MVfBcpRqfmm{YXygZZN{[Xl? NWPtfI9DSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDpcoZm[3SnZDDpckBpfW2jbjDI[WxiKFB2L2K1JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25uIFnDOVA:OC55ON88US=> NVXyboZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1PVY5QDVpPkG5OVk3QDh3PD;hQi=>
TZM-bl MWfBcpRqfmm{YXygZZN{[Xl? NWXGdVZOPzJiaILz M{Po[GFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFicF7MOE0{KGijcnLvdolv\yCUZY\ldpNmKHS{YX7zZ5JqeHSjc3WgVFEyQVNibYX0ZY51KGenbnWgbY5n\WO2ZXSgbY4hXFqPLXLsJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6NCCHQ{WwQVAvPDgQvF2= NWLMbmY{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3NFQyOzFpPkG5O|A1OTNzPD;hQi=>
TZM-bl MXnBcpRqfmm{YXygZZN{[Xl? NGrXfFZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEB4cWymLYT5dIUhUEmYMTDwUmw1NTNiaX7m[YN1\WRiaX6gWHpONWKuIHPlcIx{KGK7IHz1Z4ln\XKjc3WgZZN{[XluIFXDOVA:OC54Nd88US=> M1jkWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{C0NVMyLz5zOUewOFE{OTxxYU6=
TZM-bl M2r2[GFvfGm4aYLhcEBie3OjeR?= NELyUpI4OiCqcoO= M1nQZmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFicF7MOE0{KGijcnLvdolv\yCUZY\ldpNmKHS{YX7zZ5JqeHSjc3WgWFE3PUFibYX0ZY51KGenbnWgbY5n\WO2ZXSgbY4hXFqPLXLsJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6NCCHQ{WwQVEvODkQvF2= NEe0XYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUewOFE{OSd-MUm3NFQyOzF:L3G+
TZM-bl Mm\5RY51cX[rcnHsJIF{e2G7 M13zd|czKGi{cx?= NGCyNG5CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIIDOUFQuOyCqYYLic5JqdmdiUnX2[ZJ{\SC2cnHud4NzcXC2YYPlJHAyOTmVL2SxOlVCKG23dHHueEBo\W6nIHnu[oVkfGWmIHnuJHRbVS2kbDDj[YxteyCjZoTldkA4OiCqcoOgZpkhdHWlaX\ldoF{\SCjc4PhfUwhTUN3ME2yMlHPxE1? NXLaSIFYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3NFQyOzFpPkG5O|A1OTNzPD;hQi=>
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HepG2(2.2.15) NVvKZ|J4TnWwY4Tpc44h[XO|YYm= M{i3OVAvPzVidH:gN{B2VQ>? MWG3NkBpenN? MXXE[YNz\WG|ZTDpckBJSlZidnnyZYwhTE6DIILldIxq[2G2aX;uJIlvKGi3bXHuJGhmeEd{KEKuNk4yPSliY3XscJMh[XRiMD63OUB1dyB|IIXNJIFnfGW{IEeyJIhzeyCkeTDy[YFtNXSrbXWgVGNTKGGwYXz5d4l{NCCLQ{WwQVcvPjQQvF2= Mn3kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkG4PVEoRjJ3NE[xPFkyRC:jPh?=
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HepG22.2.15 NXLHTlJ3SW62aY\pdoFtKGG|c3H5 MkXFN{Bl[Xm| M4jHbGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDHNlIvOi5zNTDj[YxteyCjZoTldkA{KGSjeYOgZpkhdmW3dILhcEBz\WRiZInlMYJie2WmIIDsZZF2\SC{ZXT1Z5Rqd25iYYPzZZktKEmFNUC9NE4xOzoQvF2= NVrsXlRpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yOlA{OzZpPkK2NlYxOzN4PD;hQi=>
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HepG2(2.2.15) M4H6ZmFvfGm4aYLhcEBie3OjeR?= MlTSOkBl[Xm| NYLxOFFFSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHH5e|EhcW6oZXP0[YQhcW5iaIXtZY4hUGWyR{KoNk4zNjF3KTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygdoVxdGmlYYTpc44h[nlibXXhd5VzcW6pIHnueJJi[2WubIXsZZIhfmm{YXygSG5CKGOxcImgcpVu[mW{IHHmeIVzKDZiZHH5d{BjgSC{ZXHsMZRqdWVicWDDVkBu\XSqb3SsJGVEPTB:MD6wNe69VQ>? NVrxRVNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3OFg2QTBpPkK3O|Q5PTlyPD;hQi=>
HepG2(2.2.15) MnXIRY51cX[rcnHsJIF{e2G7 MYG2JIRigXN? MV3BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYh[Xm5MTDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOih{LkKuNVUqKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCC{ZYDsbYNifGmxbjDifUBu\WG|dYLpcoch\Xi2cnHj[YxtfWyjcjD2bZJidCCGTlGgZ49xgSCwdX3i[ZIh[W[2ZYKgOkBl[Xm|IHL5JJJm[WxvdHnt[UByWEOUIH3leIhw\CxiRVO1NF0xNjBzzszN MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd2OEW5NEc,Ojd5NEi1PVA9N2F-
MT4 MXTBcpRqfmm{YXygZZN{[Xl? Mn\vOUBl[Xm| NGXXbIVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCycn;0[YN1cW:wIHHnZYlve3RidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBi\nSncjC1JIRigXNiYomgUXRVKGG|c3H5MEBGSzVyPUKuNlLPxE1? NFfV[4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{e0PFU6OCd-Mke3OFg2QTB:L3G+
MT4 NXzz[FJNSW62aY\pdoFtKGG|c3H5 MnTYOUBl[Xm| NIPJe5RCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZ{IGLPSEBqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNicILveIVkfGmxbjDh[4FqdnO2II\pdpV{NWmwZIXj[YQh[3m2b4DheIhq[yCnZn\lZ5Qh[W[2ZYKgOUBl[Xm|IHL5JG1VXCCjc4PhfUwhTUN3ME24Mlgy|ryP NHjoT|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{e0PFU6OCd-Mke3OFg2QTB:L3G+
Huh7 MnP2RY51cX[rcnHsJIF{e2G7 MYfBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDsZY1qfnWmaX7lMZJme2m|dHHueEB4cWymIIT5dIUhcGWyYYTpeIl{KEJidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hUHWqNzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4he2WlcnX0[YQhTE6DIHzleoVteyCvZXHzeZJm\CCxbjDkZZkhPyCkeTDy[YFtNXSrbXWgVGNTKGGwYXz5d4l{NCCHQ{WwQVAvODV3zszN MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6MkC2PEc,OjhyOEKwOlg9N2F-
HepG2.215 MlXFRY51cX[rcnHsJIF{e2G7 MV;BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDo[ZBifGm2aYOgRkB3cXK3czDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOi5{MUWgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKH[rcnHsJGRPSSC{ZYDsbYNifGmxbjDt[YF{fXKnZDDvckBl[XliNzDifUBz\WGuIITpcYUhWEOUIHHuZYx6e2m|LDDFR|UxRTBwMd88US=> M2X3blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEiyNFY5Lz5{OEC4NlA3QDxxYU6=
Huh7 M{H2TWFvfGm4aYLhcEBie3OjeR?= M4HCd2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIxidWm4dXTpcoUuemW|aYP0ZY51KHerbHSgeJlx\SCqZYDheIl1cXNiQjD2bZJ2eyCrbn\lZ5Rm\CCrbjDoeY1idiCKdXi3JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCrboTyZYNmdGy3bHHyJGRPSSCuZY\lcJMhdWWjc4Xy[YQhd25iZHH5JFch[nlicnXhcE11cW2nIGDDVkBidmGueYPpd{whTUN3ME2wMlI{P87:TR?= NICxPVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4NlA3QCd-MkiwPFIxPjh:L3G+
HepG2.215 MWLBcpRqfmm{YXygZZN{[Xl? M4r2XGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIhmeGG2aYTpd{BDKH[rcoXzJIlv\mWldHXkJIlvKGi3bXHuJGhmeEd{LkKxOUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyZYwhTE6DIILldIxq[2G2aX;uJI1m[XO3cnXkJI9vKGSjeTC3JIJ6KHKnYXygeIlu\SCSQ2KgZY5idHm|aYOsJGVEQTB;Mt88US=> NVz4WJNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFIxPjhpPkK4NFgzODZ6PD;hQi=>
HepAD38 NFjqZW5CdnSrdnnyZYwh[XO|YYm= MoH5OUBl[Xm| NILIRZhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX7m[YN1\WRiaX6gbJVu[W5iSHXwRWQ{QCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGRPSSC{ZYDsbYNifGmxbjDh[pRmeiB3IHThfZMh[nliUmStVGNTKG2ndHjv[EwhTUN3ME2wMlA1|ryP M3yyfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEm0NVc6Lz5{OEC5OFE4QTxxYU6=
P4R5 MAGI M4XM[WZ2dmO2aX;uJIF{e2G7 NUDMcldTOiCqcoO= M{DkS2lvcGmkaYTpc44hd2ZiSFnWNUBD[UxiZHnueYNt\W:|aXTlJJJmfmW{c3WgeJJidnOlcnnweIF{\SCrbn\lZ5Rm\CCrbjDoeY1idiCSNGK1JG1CT0liY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqemGuIHnu[oVkfGmxbjDh[pRmeiB{IHjyd{BjgSCJYXzhZ5RwNVO2YYKgZoV1[S2pYXzhZ5Rwe2mmYYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NVEvO87:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN3MUW4PEc,Ojh|NUG1PFg9N2F-
P4R5 MAGI M1fhUmZ2dmO2aX;uJIF{e2G7 NU\mUWd{OiCqcoO= M4P4VWlvcGmkaYTpc44hd2ZiSFnWNUA{SiCmaX71Z4xmd3OrZHWgdoV3\XK|ZTD0doFve2O{aYD0ZZNmKGmwZnXjeIVlKGmwIHj1cYFvKFB2UkWgUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxiaX7m[YN1cW:wIHHmeIVzKDJiaILzJIJ6KEejbHHjeI8uW3SjcjDi[ZRiNWejbHHjeI9{cWSjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2zNk44|ryP NEXOSHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEO1NVU5QCd-MkizOVE2QDh:L3G+
HepG2.2.15 M4PObmFvfGm4aYLhcEBie3OjeR?= Mkj5PEBl[Xm| MUfBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDI[ZBifGm2aYOgRkB3cXK3czDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOi5{LkG1JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhTE6DIILldIxq[2G2aX;uJIFnfGW{IEig[IF6eyCkeTDy[YFtKHSrbXWg[ox2d3Knc3PlcpQhWEOUIH3leIhw\CxiSVO1NF0xNjYQvF2= M3f4V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEm0NlUzLz5{OES5OFI2OjxxYU6=
MT4 M2i5cWFvfGm4aYLhcEBie3OjeR?= NX35WZJEPSCmYYnz MU\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGlXOSB|QjDpcoZm[3SnZDDpckBpfW2jbjDNWFQh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGGodHXyJFUh\GG7czDifUBOXFRiYYPzZZktKEWFNUC9Nk42PM7:TR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3OUK0Okc,Ojh4NUmyOFY9N2F-
HepG2(2.2.15) MmrBRY51cX[rcnHsJIF{e2G7 NFP0bmI3KGSjeYO= MmrRRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGG7d{GgbY5n\WO2ZXSgbY4hcHWvYX6gTIVxTzJqMj6yMlE2MSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxicnXwcIlk[XSrb36gZpkhdWWjc4XybY5oKGmwdILhZ4VtdHWuYYKgeolz[WxiRF7BJINweHliboXtZoVzKGGodHXyJFYh\GG7czDifZIh[nlicnXhcE11cW2nIIHQR3Ih[XO|YYmsJGVEPTB;MD6wNFg5P87:TR?= NVrneHpDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFIxPjdpPkK4OlgzODZ5PD;hQi=>
HepG2(2.2.15) M{Wwb2FvfGm4aYLhcEBie3OjeR?= NVPLSWk1PiCmYYnz NYfVdZFZSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHH5e|EhcW6oZXP0[YQhcW5iaIXtZY4hUGWyR{KoNk4zNjF3KTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygdoVxdGmlYYTpc44h[nlibXXhd5VzcW6pIHX4eJJi[2WubIXsZZIhfmm{YXygSG5CKGOxcImgcpVu[mW{IHHmeIVzKDZiZHH5d{BjgSC{ZXHsMZRqdWVicWDDVkBu\XSqb3SsJGVEPTB;MD6wNu69VQ>? NHW0c4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4NlA3Pyd-Mki2PFIxPjd:L3G+
HepAD38 M17UU2FvfGm4aYLhcEBie3OjeR?= NIflSY5CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX7m[YN1\WRiaX6gbJVu[W5iSHXwRWQ{QCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbY51emGlZXzseYxieiC4aYLhcEBFVkFibHX2[Ywh[nliUmStVGNTKG2ndHjv[EwhTUN3MEywMlAy|ryP MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ6OEK4NEc,Ojh4OEiyPFA9N2F-
HepAD38 MoGzRY51cX[rcnHsJIF{e2G7 Moi0RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGmwZnXjeIVlKGmwIHj1cYFvKEincFHEN|gh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKHKncHzpZ4F1cW:wIHL5JHJVNVCFUjDt[ZRpd2RuIFXDOVA:OC5yNN88US=> NEDodZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PFI5OCd-Mki2PFgzQDB:L3G+
HepAD38 MYXBcpRqfmm{YXygZZN{[Xl? NFrGVmhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX7m[YN1\WRiaX6gbJVu[W5iSHXwRWQ{QCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[Zh1emGlZXzseYxieiC4aYLhcEBFVkFibHX2[Ywh[nliUmStVGNTKG2ndHjv[EwhTUN3ME2wMlA1|ryP Mn:xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4OEiyPFAoRjJ6Nki4NlgxRC:jPh?=
HepAD38 MVHBcpRqfmm{YXygZZN{[Xl? MoLDRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGmwZnXjeIVlKGmwIHj1cYFvKEincFHEN|gh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKHKncHzpZ4F1cW:wIHL5JHJVNVCFUjDt[ZRpd2RuIFXDPVA:OC5|zszN M{\ub|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4NlgxLz5{OE[4PFI5ODxxYU6=
HepAD38 NGDGbYpCdnSrdnnyZYwh[XO|YYm= M3f5WGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDBSFM5KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDlfJRz[WOnbHz1cIFzKH[rcnHsJGRPSSCuZY\lcEBjgSCUVD3QR3IhdWW2aH;kMEBGSzlyPUCuN:69VQ>? NYnIPYZ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFgzQDBpPkK4Olg5OjhyPD;hQi=>
HepAD38 MmjHRY51cX[rcnHsJIF{e2G7 NHn4[WJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX7m[YN1\WRiaX6gbJVu[W5iSHXwRWQ{QCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbY51emGlZXzseYxieiC4aYLhcEBFVkFibHX2[Ywh[nliUmStVGNTKG2ndHjv[EwhTUN7ME2wMlfPxE1? Mm\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4OEiyPFAoRjJ6Nki4NlgxRC:jPh?=
SupT1 NV3PfnduSW62aY\pdoFtKGG|c3H5 MoSzOFghcHK| NUXmbIplSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhXlOYLVegdJNmfWSxdInw[YQhUEmYMTDpcoZm[3SnZDDpckBpfW2jbjDTeZBVOSClZXzsd{Bi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwQVAvOc7:TR?= MlTmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyMU[xN|EoRjJ7MEG2NVMyRC:jPh?=
HepG2(2.2.15) MV;BcpRqfmm{YXygZZN{[Xl? MkLKOkBl[Xm| MYLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYhcW6oZXP0[YQhcW5iaIXtZY4hUGWyR{KoNk4zNjF3KTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJGRPSSC{ZYDsbYNifGmxbjDh[pRmeiB4IHThfZMh[nliUmStVGNTKG2ndHjv[EwhUUN3MEywMlHPxE1? MkLTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{OEi5OFMoRjJ7Mki4PVQ{RC:jPh?=
A549 M3;oPWFvfGm4aYLhcEBie3OjeR?= NVLwNI46SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTD2bZJqd26|IHTldol3\WRiZoLvcUBJUVZxVmPHMWchd3JiSFnWM2hCKH[rcnnvcpMhcW6oZXP0[YQhOjl|VDDj[YxteyC3c3nu[{BpfW2jbjDBOVQ6KGOnbHzzJIF{KHSjcnfleEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIIXzbY5oKGOxbYDveY5lKHC{ZT3tbZhm\CC4aYLpc45{KG[xbHzve4VlKGK7IHnuZ5Vj[XSrb36ge4l1cCC2YYLn[ZQh[2WuLDDJR|UxRTBwNUJOwG0> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ7OUmyOEc,Ojl4OUm5NlQ9N2F-
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Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 229.26
Formula

C8H11N3O3S

CAS No. 134678-17-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(OC(S1)CO)N2C=CC(=NC2=O)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04133012 Recruiting Other: Samplings HIV-1 Infection ANRS Emerging Infectious Diseases|ViiV Healthcare February 10 2020 Not Applicable
NCT03869944 Active not recruiting Drug: Lamivudine Oral Solution HIV-1 ANRS Emerging Infectious Diseases|Institut National de la Santé Et de la Recherche Médicale France|Centre Muraz December 4 2019 Phase 2
NCT04034862 Active not recruiting Drug: Treatment simplification (dolutegravir lamivudine) HIV Infections University of Liege October 1 2019 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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