Lamivudine (BCH-189)

Catalog No.S1706 Synonyms: GR109714X

For research use only.

Lamivudine (BCH-189, GR109714X) is a potent nucleoside analog reverse transcriptase inhibitor, used for treatment of chronic HBV and HIV/AIDS. It works by blocking the HIV reverse transcriptase and hepatitis B virus polymerase.

Lamivudine (BCH-189) Chemical Structure

CAS No. 134678-17-4

Selleck's Lamivudine (BCH-189) has been cited by 11 Publications

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Biological Activity

Description Lamivudine (BCH-189, GR109714X) is a potent nucleoside analog reverse transcriptase inhibitor, used for treatment of chronic HBV and HIV/AIDS. It works by blocking the HIV reverse transcriptase and hepatitis B virus polymerase.
Targets
Reverse transcriptase [1]
In vitro

Lamivudine’s anti- HBV activity, like its anti-HIV activity, has been shown to depend on the ability of LMV-TP to serve as both substrate and inhibitor of the DNA- and RNA-dependent polymerase activities of the HBV P gene product. Lamivudine owes its activity to the remarkably broad substrate specificity of deoxycytidine kinase and the unusual substrate preference of the HBV polymerases for dNTPs with the unnatural L-conformation, whereas the anti-HBV activity of PCV appears to depend on several factors including optimal phosphorylation (sufficient for antiviral activity but not cytotoxicity) by key cellular enzymes, the long intracellular half-life of PCV-TP and the ability of PCV-TP to inhibit the HBV RT priming reaction as well as RT and DNA polymerase activity. [1] Lamivudine and Penciclovir inhibits duck hepatitis B virus (DHBV) replication to a comparable extent when used alone, and in combination, the two nucleoside analogs acts synergistically over a wide range of clinically relevant concentrations. Lamivudine combined with Penciclovir is more effective in reducing the normally recalcitrant viral covalently closed circular (CCC) DNA form of DHBV than either drug alone. [2] Lamivudine inhibits p24 antigen production by HIV-I in PBMC, with ED50s ranging from 0.07 μM to 0.2 μM. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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PBMC NUPtdZo4TnWwY4Tpc44h[XO|YYm= M4faS2NwdmOnboTyZZRqd25icnXxeYlz\WRiZn;yJIFvfGm4aYLhcEBi[3Srdnn0fUBqdiCSQl3DJEhpfW2jbjDw[ZJqeGincnHsJIJtd2:mIH3vco9vfWOuZXHyJINmdGy|KTDpcoZm[3SnZDD3bZRpKEiLVj2xJJN1emGrbjDMRXYtKEWFNUC9NE4xODF6zszN MnjJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDRzMEm3OUc,QDRzMEm3OVww[T5?
PBMC NXrjNpdITnWwY4Tpc44h[XO|YYm= NWfQZnR7S2:wY3XueJJifGmxbjDy[ZF2cXKnZDDmc5Ih[W62aY\pdoFtKGGldHn2bZR6KGmwIGDCUWMhMGi3bXHuJJBmemmyaHXyZYwh[myxb3SgcY9vd263Y3zlZZIh[2WubIOpJIlv\mWldHXkJJdqfGhiSFnWMVEhe3S{YXnuJGxCXixiRVO1NF0xNjJzzszN NWnFco5lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOESxNFk4PSd-OESxNFk4PTxxYU6=
PBMC M2\LcmFvfGlvSFnWMVEh[XO|YYm= NVHPOGdpSW62aT3ITXYuOSCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJJBmemmyaHXyZYwh[myxb3SgcY9vd263Y3zlZZIhMFCETTmgZ4VtdHNiaX7m[YN1\WRid3n0bEBJUVZvMTDzeJJicW5iTFHWMEBGSzVyPUCuNFAyQM7:TR?= NELmXVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NEKzOVkyLz56NEKzOVkyRC:jPh?=
PBMC NIXF[FBCdnSrLVjJWk0yKGG|c3H5 M3zoNWFvfGlvSFnWMVEh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDw[ZJqeGincnHsJIJtd2:mIH3vco9vfWOuZXHyJEhRSk1rIHPlcIx{KGmwZnXjeIVlKHerdHigTGlXNTFic4TyZYlvKEyDVjygSWM2OD1yLkC2{txO MkXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDR{M{W5NUc,QDR{M{W5NVww[T5?
PBMC NYXy[mRGSW62aT3ITXYuOSCjc4PhfS=> NFvobmVCdnSrLVjJWk0yKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDtc45wdnWlbHXhdkApWEKPKTDj[YxteyCrbn\lZ5Rm\CC5aYToJGhKXi1zIIP0doFqdiCOQW[sJGVEPTB;MD6yNe69VQ>? M{n6WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh2MkO1PVEoRjh2MkO1PVE9N2F-
PBMC M4\IU2FvfGlvSFnWMVEh[XO|YYm= MXPBcpRqNUiLVj2xJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5icHXybZBp\XKjbDDicI9w\CCvb37vcpVkdGWjcjCoVGJOMSClZXzsd{Bqdm[nY4Tl[EB4cXSqIFjJWk0yKHO2cnHpckBNSVZuIFXDOVA:OTBwMd88US=> NX\lUm9VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOESyN|U6OSd-OESyN|U6OTxxYU6=
2.2.15 NGXtUmJHfW6ldHnvckBie3OjeR?= M3vTdGlvKH[rdILvJIVn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiaHXwZZRqfGm|IFKgeolzfXNid3HzJIRmfGW{bXnu[YQhcW5iMj6yMlE2KGOnbHzzMEBGSzVyPUCuNFA5|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86OzBzNkW5K|46OzBzNkW5QE9iRg>?
PBMC NUfsUGtXSW62aT3ITXYuOSCjc4PhfS=> NXjuRXA3UW5idnn0do8h[W62aT3ITXYh[WO2aY\peJkhcW5icHXybZBp\XKjbDDicI9w\CCvb37vcpVkdGWjcjDj[YxteyxiRVO1NF0xNjB5zszN NHjtWWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97M{CxOlU6Lz57M{CxOlU6RC:jPh?=
2.2.15 M2K1U2FvfGm4aYLhcEBie3OjeR?= NXXRWmpqSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUGWyYYTpeIl{KEJidnnyeZMhMEiEVjmgbY4hOi5{LkG1JINmdGy|LDDFR|UxRTBwMEC4{txO MnnFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTFyNUK3PVUoRjFzMEWyO|k2RC:jPh?=
PBMC NVm5flhNSW62aY\pdoFtKGG|c3H5 MlfVRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTY1ufW6xZHXmbYNq\W6leTD2bZJ2eyC2eYDlMVEhMEiLVj2xLUBqdiCyZYLpdIhmemGuIHLsc49lKG2xbn;ueYNt\WG{IHPlcIx{NCCHQ{WwQVAvODgQvF2= NUDv[VJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGwOVI4QTVpPkGxNFUzPzl3PD;hQi=>
H9 M1vXeGFvfGm4aYLhcEBie3OjeR?= MW\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnuJIh2dWGwIFi5JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBjgSCob4LtZZpidi2kYYPl[EBkd264ZX70bY9v[WxiY3;sc5JqdWW2cnnjJJRm[2iwaYH1[UwhTUN3ME2wMlA3|ryP M1vxT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNEOwNFE6Lz5zMUSzNFAyQTxxYU6=
H9 NUTGTGU{SW62aY\pdoFtKGG|c3H5 NVf0UXUxSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDSSkBqdiCqdX3hckBJQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYomg[o9zdWG8YX6tZoF{\WRiY3;ueoVvfGmxbnHsJINwdG:{aX3leJJq[yC2ZXPocolyfWVuIFXDOVA:OC5yNt88US=> NVTsPGFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG0N|AxOTlpPkGxOFMxODF7PD;hQi=>
PBMC M1KyRWFvfGm4aYLhcEBie3OjeR?= M1r6emFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXi1zeIjCVnUhdXW2YX70JJN1emGrbjDpckBpfW2jbjDQRm0h[2WubIOsJGVEQTB;MD6wPO69VQ>? Mn7vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ|OEOwNVQoRjF{M{izNFE1RC:jPh?=
PBMC M2TH[mZ2dmO2aX;uJIF{e2G7 M371SWFkfGm4aYT5JIFo[Wmwc4SgUIFucX[3ZHnu[U1z\XOrc4ThcpQhfmm{dYOgLGhKXi1zWGjCVnUqKGmwIHj1cYFvKFCETTDj[YxteyxiZYjwdoV{e2WmIHHzJGVEPTBuIFXDOVA:OC5yM{ZOwG0> MmfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6NUK3OVUoRjF{OEWyO|U2RC:jPh?=
PBMC MnvVSpVv[3Srb36gZZN{[Xl? MlHVTY4hfmm2cn:gZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IITo[UBJUVZvMTDMRVEhemWybHnjZZRqd25ib36gVGJOS3NiY3XscEApeGW{aYDo[ZJidCCkbH;v[EBud26xboXjcIVieiClZXzsd{ktKEWFNUC9NE4xOc7:TR?= NIP1PIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMki1Nlk1Oyd-MUK4OVI6PDN:L3G+
PBMC NXqze24{TnWwY4Tpc44h[XO|YYm= MljMSYZn\WO2aY\lJINwdmOnboTyZZRqd25idH:gbY5pcWKrdDDITXYuOSCOQVmgZ5l1d3CjdHjpZ4l1gSCrbjDQRm1EKGOnbHzzJJdieyCmZYTldo1qdmWmIHnuJJZqfHKxLDDFR|UxRTBwMEGxOu69VQ>? MkG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR7N{G4PVgoRjF2OUexPFk5RC:jPh?=
HepG2.2.15 MoDxSpVv[3Srb36gZZN{[Xl? M3TDcmVn\mWldHn2[UBkd26lZX70doF1cW:wIITvJIlvcGmkaYSgbIVx[XSrdHnzJGIhfmm{dYOgZ5l1d3CjdHjpZ4l1gSCrbjDI[ZBIOi5{LkG1JINmdGy|IIfhd{Bl\XSncn3pcoVlKGmwII\peJJwNCCHQ{WwQVAvOs7:TR?= M1jCV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2OUexPFk5Lz5zNEm3NVg6QDxxYU6=
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PBMC Ml\xRY51cX[rcnHsJIF{e2G7 MYnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGlXKH[rcoXzLJh5SlKXKTDpckBJfW2jbjDw[ZJqeGincnHsJIJtd2:mIH3vco9vfWOuZXHyJEhRSk1rIHPlcIx{NCCHQ{mwQVAvODkQvF2= NHzPSng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUCyO|g2PCd-MUWwNlc5PTR:L3G+
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CEM-SS NInKS2FCdnSrdnnyZYwh[XO|YYm= NXLIN|M3PiCmYYnz M1HBTWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gR2VONVOVIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{dYOtbY5lfWOnZDDjfZRweGG2aHnjJIVn\mWldDDh[pRmeiB4IHThfZMh[nliTWTTJIF{e2G7LDDFSFUxRTBwM{dOwG0> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJzN{K4NUc,OTV{MUeyPFE9N2F-
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2.2.15 NUjEdoV4TnWwY4Tpc44h[XO|YYm= NWnvV4JoUW6qaXLpeIlwdiCxZjDzfY51cGW|aYOgc4Yh\Xi2cnHj[YxtfWyjcjD2bZJqd25iRF7BJJJmdGWjc3WgbY4h[3WudIXy[YQhcHWvYX6gbIVx[XSxYnzhd5RwdWFiMj6yMlE2KGOnbHzzMEBGSzlyPUCuNVUy|ryP MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzM{exN{c,OTZ{MUO3NVM9N2F-
2.2.15 NF7uVYVHfW6ldHnvckBie3OjeR?= NV3Yco44UW6qaXLpeIlwdiCxZjDzfY51cGW|aYOgc4YhcW62cnHj[YxtfWyjcjDIRnYhTE6DIILldIxq[2G2aY\lJIlvfGW{bXXkbYF1\XNiaX6gZ5VtfHW{ZXSgbJVu[W5iaHXwZZRw[myjc4TvcYEhOi5{LkG1JINmdGy|LDDFR|UxRTBwMkWx{txO Mkm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{MUO3NVMoRjF4MkGzO|E{RC:jPh?=
2.2.15 MnHYSpVv[3Srb36gZZN{[Xl? MYTJcohq[mm2aX;uJI9nKHO7boTo[ZNqeyCxZjDpcpRz[WOnbHz1cIFzKEiEVjDEUmEhemWybHnjZZRqfmViaX70[ZJu\WSrYYTld{BqdiCldXz0eZJm\CCqdX3hckBp\XCjdH;icIF{fG:vYTCyMlIvOTViY3XscJMtKEWFOUC9NE43PDkQvF2= NEHjeGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxN|cyOyd-MU[yNVM4OTN:L3G+
2.2.15 NXvRbGZ2TnWwY4Tpc44h[XO|YYm= NXn5NJo1UW6qaXLpeIlwdiCxZjD3bYxlKHS7cHWgTGJXKHS{YX7z[oVkfGWmIHnuJIh2dWGwIHjldIF1d2KuYYP0c41iKDJwMj6xOUBk\WyuczygSWM2OD1{LkJOwG0> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV|OUO5N{c,OTZ3M{mzPVM9N2F-
hepatoma 2.2.15 MULBcpRqfmm{YXygZZN{[Xl? MUTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGJXKGmwIHj1cYFvKGincHH0c41iKDJwMj6xOUBk\WyuczygSWM2OD1{LkJOwG0> MmfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5NUmxNVIoRjF4N{W5NVEzRC:jPh?=
2.2.15 MnXySpVv[3Srb36gZZN{[Xl? NG\RSXNKdmirYnn0bY9vKG:oIFjCWkBz\XCuaXPheIlwdiCrbjCyMlIvOTViY3XscJMh[nliRF7BJIh6[nKrZHn6ZZRqd25iYYPzZZktKEWFNUC9NE4xOs7:TR?= MmrVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyMES3NlYoRjF5MEC0O|I3RC:jPh?=
MDCK2 NHGzOWxHfW6ldHnvckBie3OjeR?= MlThNVAhfU1? NYT3TGRWUW6qaXLpeIlwdiCxZjDoeY1idiCPUmCyJIV5eHKnc4Pl[EBqdiCPRFPLNkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCrboTyZYNmdGy3bHHyJGNOTiCobIXvdoV{[2WwY3WgZZQhOTBidV2gZpkhS02IRFGgZZN{[Xl? NWfiS4V6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexO|I{OTFpPkG3NVczOzFzPD;hQi=>
MDCK2 NHK5Xm9HfW6ldHnvckBie3OjeR?= M1\hb|ExKHWP Mk\XTY5pcWKrdHnvckBw\iCqdX3hckBOWlB|IHX4dJJme3OnZDDpckBOTEONMjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBqdnS{YXPlcIx2dGG{IFPNSkBndHWxcnXzZ4Vv[2ViYYSgNVAhfU1iYomgR21HTEFiYYPzZZk> Mn\JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzN{KzNVEoRjF5MUeyN|EyRC:jPh?=
MDCK2 MmDGSpVv[3Srb36gZZN{[Xl? NVHoe3dDOTBidV2= NUPNVYJyUW6qaXLpeIlwdiCxZjDoeY1idiCPUmCxJIV5eHKnc4Pl[EBqdiCPRFPLNkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCrboTyZYNmdGy3bHHyJGNOTiCobIXvdoV{[2WwY3WgZZQhOTBidV2gZpkhS02IRFGgZZN{[Xl? NVT3UGZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexO|I{OTFpPkG3NVczOzFzPD;hQi=>
Huh7 MVLGeY5kfGmxbjDhd5NigQ>? MkfLTY5pcWKrdHnvckBw\iC5aXzkJJR6eGViSFLWJJJmeGyrY3H0bY9vKGmwIFj1bFch[2WubIOsJGVEPTB;Md88US=> M1vSfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{exPFI4Lz5zN{O3NVgzPzxxYU6=
PBMC NUTIepVxSW62aY\pdoFtKGG|c3H5 NY\rOpJzSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDMRWkhcW5iaIXtZY4hWEKPIHPlcIx{NCCHQ{WwQVAvODJ4zszN MoXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MEO5PVYoRjF5NECzPVk3RC:jPh?=
PBMC NYrWTpdMSW62aY\pdoFtKGG|c3H5 M2fvbGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIlvKGi3bXHuJHBDVSClZXzsd{whTUN7ME2wMlEz|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyM{m5Okc,OTd2MEO5PVY9N2F-
HepG2.2.15 NGHMfppCdnSrdnnyZYwh[XO|YYm= M3TaWmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbjDI[ZBIOi5{LkG1JINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGW6dILhZ4VtdHWuYYKgeolz[WxiRF7BJIxmfmWuIHL5JHJVNVCFUjygSWM2OD1yLkJOwG0> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyNECwOkc,OTd2MESwNFY9N2F-
Huh7 NG\FcYJCdnSrdnnyZYwh[XO|YYm= M{HuVlUh\GG7cx?= MX;BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYh\2Wwb4T5dIUhTCC5aYToJJJmfmW{c3WgeJJidnOlcnnweIF{\SCOOEDJJI12fGG2aX;uJIlvKGi3bXHuJGh2cDdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC1JIRigXNuIFXDOVA:OC5yM988US=> NHPhOmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SzPFA1Pyd-MUe0N|gxPDd:L3G+
Huh7 Mn3QRY51cX[rcnHsJIF{e2G7 NUKycVU{PSCmYYnz NWfyTYtVSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKnQXet[IVncWOrZX70JGhDXiCpZX7veJlx\SCGIIfpeIgheHKnY3;y[UBIOTh7NlGgcZV1[XSrb36gZY5lKHKndnXyd4UhfHKjboPjdolxfGG|ZTDMPFBKKG23dHH0bY9vKGmwIHj1cYFvKEi3aEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA2KGSjeYOsJGVEPTB;MD6wN:69VQ>? NYHxXGJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0N|gxPDdpPkG3OFM5ODR5PD;hQi=>
Huh7 M3zsO2FvfGm4aYLhcEBie3OjeR?= MoHwOUBl[Xm| NULxe21bSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHflco91gXCnIFSgbY4hcHWvYX6gTJVpPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEWg[IF6eyxiRVO1NF0xNjRzzszN MmXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2M{iwOFcoRjF5NEO4NFQ4RC:jPh?=
Huh7 NGnvbGNCdnSrdnnyZYwh[XO|YYm= M4OwSVUh\GG7cx?= M2XXSGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhD\UGpLXTl[olkcWWwdDDIRnYh\2Wwb4T5dIUhTCC5aYToJJBz\WOxcnWgS|E5QT[DIH31eIF1cW:wIHnuJIh2dWGwIFj1bFch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB3IHThfZMtKEWFNUC9NE41Ps7:TR?= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR|OEC0O{c,OTd2M{iwOFc9N2F-
HepG2(2.2.15) NVOwe2hqSW62aY\pdoFtKGG|c3H5 NIfCN5NCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX6gZZl4KHerbHSgeJlx\SCKZYDHNkgzNjJwMUWpJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhTE6DIILldIxq[2G2aX;uMEBKSzVyPUCuNFXPxE1? NHv5THg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S4PFgyPyd-MUe0PFg5OTd:L3G+
HepG2(2.2.15) NUC0d4c6TnWwY4Tpc44h[XO|YYm= NHXWW5hKdmirYnn0bY9vKG:oIFjCWkBxem:vb4TldkBqdiCqdX3hckBJ\XCJMjiyMlIvOTVrIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2wMlA5|ryP M2foWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEi4PFE4Lz5zN{S4PFgyPzxxYU6=
HepW10 NEW2OXZCdnSrdnnyZYwh[XO|YYm= MV3BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYhcW5iYXTybYFugWOrbj3y[ZNqe3SjboSge4lt\CC2eYDlJGhmeFdzMDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJGRPSSC{ZYDsbYNifGmxbjygTWM2OD1yLkJOwG0> M{L1fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEi4PFE4Lz5zN{S4PFgyPzxxYU6=
HBV-Met MVjBcpRqfmm{YXygZZN{[Xl? MkXKRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGmwIH3veZNmKGG7dzD3bYxlKHS7cHWgTGJXNU2ndDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJGRPSSC{ZYDsbYNifGmxbjygTWM2OD1yLkNOwG0> M4fEXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEi4PFE4Lz5zN{S4PFgyPzxxYU6=
HepG2.2.15 NHjUTYlHfW6ldHnvckBie3OjeR?= MYW4JIRigXN? MWHJcohq[mm2aX;uJI9nKGO7dH;wcIF{dWmlIFjCWkBFVkFic4nueIhme2m|IHnuJGhmeEd{LkKuNVUh[2WubIOgZYZ1\XJiODDkZZl{NCCLQ{WwQVAvOzkQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR7OUi4PUc,OTd2OUm4PFk9N2F-
PBMC MmTLSpVv[3Srb36gZZN{[Xl? NIXNNI4{ODBibXe= NGe0VWQ4KGSjeYO= M1\mPWNu[XhiaX6gTGlXNWmwZnXjeIVlKGi3bXHuJIF{e2W|c3XkJIF{KEyjbXn2eYRqdmVibX;uc5Bpd3OyaHH0[UBt\X[nbDDpckBRSk2FIHH0JFMxOCCvZzDRSEBnd3JiNzDkZZl{KG2nYYP1doVlKHCncjCxNEc3KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIIrp[I93fWSrbnWsJGNu[Xh;MD6wNFAxODB6zszN NWnxO5dFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlQ{OjhpPkG3OlY1OzJ6PD;hQi=>
PBMC NWL4ZZAzTnWwY4Tpc44h[XO|YYm= MXqzNFAhdWd? M3zjR|ch\GG7cx?= NU\EZZZlS22jeDDpckBJUVZvaX7m[YN1\WRiaIXtZY4h[XO|ZYPz[YQh[XNiTHHtbZZ2\GmwZTDkbZBpd3OyaHH0[UBt\X[nbDDpckBRSk2FIHH0JFMxOCCvZzDRSEBnd3JiNzDkZZl{KG2nYYP1doVlKHCncjCxNEc3KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIIrp[I93fWSrbnWsJGNu[Xh;MD6wNFAxODR2zszN MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ4NEOyPEc,OTd4NkSzNlg9N2F-
PBMC M3mzXGZ2dmO2aX;uJIF{e2G7 NVWyRZpFOzByIH3n Mlu1O{Bl[Xm| NWLWW4gxS22jeDDpckBJUVZvaX7m[YN1\WRiaIXtZY4h[XO|ZYPz[YQh[XNiTHHtbZZ2\GmwZTD0dolxcG:|cHjheIUhdGW4ZXygbY4hWEKPQzDheEA{ODBibXegVWQh\m:{IEeg[IF6eyCvZXHzeZJm\CCyZYKgNVAoPiClZXzsd{BqdiCycnXz[Y5k\SCxZjD6bYRwfnWmaX7lMEBEdWG6PUCuNFAxODB2ON88US=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ4NEOyPEc,OTd4NkSzNlg9N2F-
MAGIC-5A M1PTdmFvfGm4aYLhcEBie3OjeR?= NF;mZnU1OCCqcoO= M3T6fGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIF7MOE0{KHC{b3T1Z4VlKG[{b32g[pVtdCCuZX7neIgheFJ7ZHXseIFCeGFiaX7m[YN1\WRiaX6gbJVu[W5iTVHHTWMuPUFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBN[WNtIH\vZ4kh\XiycnXzd4lwdiCjZoTldkA1OCCqcoOsJGVEPTB;MD64PO69VQ>? NXjHNIRWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Olc6OTNpPkG3PVY4QTF|PD;hQi=>
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MT2 M{PnfGFvfGm4aYLhcEBie3OjeR?= M{LyNlUh\GG7cx?= Moj1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgNE4xPSCPT1mge4lt\CC2eYDlJGhKXjFiTly0MVMhcW6oZXP0[YQhcW5iMjDodk1xemW2cnXheIVlKGi3bXHuJG1VOiClZXzsd{Bu\WG|dYLl[EBi\nSncjC1JIRigXNiYomgUXRUKGG|c3H5MEBGSzVyPUOuNlQ4|ryP MoTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|MU[1NlEoRjF6M{G2OVIyRC:jPh?=
HepG2(2.2.15) Ml3PRY51cX[rcnHsJIF{e2G7 MX[yOEBpenN? MX3BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYhcW5iaIXtZY4hUGWyR{KoNk4zNjF3KTDj[YxteyCjc4Pld5Nm\CCjczDlfJRz[WOnbHz1cIFzKEiEVjD2bZJqd25iRF7BJIxmfmWuIHHmeIVzKDJ2IHjyd{whTUN3ME2wMlA1QM7:TR?= NEX6Nmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC3NlY6PCd-MUmwO|I3QTR:L3G+
HepG2(2.2.15) MWrBcpRqfmm{YXygZZN{[Xl? MmLuNlQhcHK| NVy4Vm1PSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHnuJIh2dWGwIFjldGczMDJwMj6xOUkh[2WubIOgZZN{\XO|ZXSgZZMhemWybHnjZZRqd25iaX70[ZJu\WSrYYTld{Bi\nSncjCyOEBpenNuIFXDOVA:OC5zNd88US=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB5Mk[5OEc,OTlyN{K2PVQ9N2F-
Huh7 MWfBcpRqfmm{YXygZZN{[Xl? MYG0JIRigXN? NX61bnIxSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhdGGvaY\1[Ilv\S2{ZYPpd5RidnRid3ns[EB1gXCnIFjCWkBqdiCqdX3hckBJfWh5IHPlcIx{KGGodHXyJFQh\GG7czDifUBqdnS{YXPlcIx2dGG{IFjCWkBFVkFicnXwcIlk[XSrb36gZZN{[XluIFXDOVA:OC5{zszN NYGyXlZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwO|I3QTRpPkG5NFczPjl2PD;hQi=>
Huh7 NGKzTFBCdnSrdnnyZYwh[XO|YYm= NFX0Oo81KGSjeYO= M3SyOGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIxidWm4dXTpcoUuemW|aYP0ZY51KEiEVjDy[ZZmenOnIITyZY5{[3KrcIThd4UhOjN4VDDteZRidnRiaX6gbJVu[W5iSIXoO{Bk\WyuczDh[pRmeiB2IHThfZMh[nliaX70doFk\WyudXzhdkBJSlZiRF7BJJJmeGyrY3H0bY9vKGG|c3H5MEBGSzVyPUCuN:69VQ>? M{\FUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
Huh7 MlHsRY51cX[rcnHsJIF{e2G7 M33SeVQh\GG7cx?= NELpbnFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBi\GWob4\pdk1z\XOrc4ThcpQhf2muZDD0fZBmKEiEVjDpckBpfW2jbjDIeYg4KGOnbHzzJIFnfGW{IESg[IF6eyCkeTDpcpRz[WOnbHz1cIFzKEiEVjDEUmEhemWybHnjZZRqd25iYYPzZZktKEWFNUC9NU4{|ryP M4TJ[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
Huh7 MkHMRY51cX[rcnHsJIF{e2G7 NXTkN2R2PCCmYYnz NVjzO29NSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qh[WSnZn;2bZIuemW|aYP0ZY51KEiEVjDy[ZZmenOnIITyZY5{[3KrcIThd4UhVTJyNG[gcZV1[W62IHnuJIh2dWGwIFj1bFch[2WubIOgZYZ1\XJiNDDkZZl{KGK7IHnueJJi[2WubIXsZZIhUEKYIFTORUBz\XCuaXPheIlwdiCjc4PhfUwhTUN3ME2xMlXPxE1? NVfleppCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwO|I3QTRpPkG5NFczPjl2PD;hQi=>
Huh7 Mn7FRY51cX[rcnHsJIF{e2G7 NInwSlY1KGSjeYO= MVLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDh[IVnd3[rcj3y[ZNqe3SjboSgTGJXKHKndnXyd4UhfHKjboPjdolxfGG|ZTDMNVgxVSCvdYThcpQhcW5iaIXtZY4hUHWqNzDj[YxteyCjZoTldkA1KGSjeYOgZpkhcW62cnHj[YxtfWyjcjDIRnYhTE6DIILldIxq[2G2aX;uJIF{e2G7LDDFR|UxRTFwNt88US=> M4fMW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
Huh7 M{XMPGFvfGm4aYLhcEBie3OjeR?= NETLO|A1KGSjeYO= NILVOZlCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBi\GWob4\pdk1z\XOrc4ThcpQhUEKYIILleoVze2VidILhcpNkemmydHHz[UBNVS:{ZY\ldpNmKHS{YX7zZ5JqeHSjc3WgUXYh\G:3YnzlJI12fGGwdDDpckBpfW2jbjDIeYg4KGOnbHzzJIFnfGW{IESg[IF6eyCkeTDpcpRz[WOnbHz1cIFzKEiEVjDEUmEhemWybHnjZZRqd25iYYPzZZktKEWFNUC9NU43|ryP M{HBe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEeyOlk1Lz5zOUC3NlY6PDxxYU6=
Huh7 M3vCXmFvfGm4aYLhcEBie3OjeR?= NUe5VnlxPCCmYYnz M2O0N2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIFl\W[xdnnyMZJme2m|dHHueEBJSlZicnX2[ZJ{\SC2cnHud4NzcXC2YYPlJG0zODSLIH31eIFvfCCrbjDoeY1idiCKdXi3JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCrboTyZYNmdGy3bHHyJGhDXiCGTlGgdoVxdGmlYYTpc44h[XO|YYmsJGVEPTB;MT64{txO NHPFOVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC3NlY6PCd-MUmwO|I3QTR:L3G+
Huh7 M2Dh[mFvfGm4aYLhcEBie3OjeR?= MnHMOEBl[Xm| MnXrRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgZYRm\m:4aYKtdoV{cXO2YX70JGhDXiC{ZY\ldpNmKHS{YX7zZ5JqeHSjc3WgNlM3XCCvdYThcpQhcW5iaIXtZY4hUHWqNzDj[YxteyCjZoTldkA1KGSjeYOgZpkhcW62cnHj[YxtfWyjcjDIRnYhTE6DIILldIxq[2G2aX;uJIF{e2G7LDDFR|UxRTdwN988US=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB5Mk[5OEc,OTlyN{K2PVQ9N2F-
Huh7 Mlz6RY51cX[rcnHsJIF{e2G7 M{fmVlQh\GG7cx?= M4jvbmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIxidWm4dXTpcoUuemW|aYP0ZY51KEiEVjDy[ZZmenOnIITyZY5{[3KrcIThd4UhVDF6MF2gcZV1[W62IHnuJIh2dWGwIFj1bFch[2WubIOgZYZ1\XJiNDDkZZl{KGK7IHnueJJi[2WubIXsZZIhUEKYIFTORUBz\XCuaXPheIlwdiCjc4PhfUwhTUN3ME2xNO69VQ>? MoLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyN{K2PVQoRjF7MEeyOlk1RC:jPh?=
HuH7 NVzZNolGSW62aY\pdoFtKGG|c3H5 MoTYNlQhcHK| MXjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGJXKGmwZnXjeIVlKGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygeJJidnOrZX70JJRz[W6|ZnXjeIlwdiCjZoTldkAzPCCqcoOsJGVEPTB;MD6y{txO MnflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|OUi2OFgoRjF7M{m4OlQ5RC:jPh?=
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HuH7 NH\3dolCdnSrdnnyZYwh[XO|YYm= MX:yOEBpenN? NGqwdmJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaHHyZo9zcW6pIGLORUBxd2y7bXXyZZNmKE5{M{\UJI12fGGwdDDn[Y5mKGmwZnXjeIVlKGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygeJJidnOrZX70JJRz[W6|ZnXjeIlwdiCjZoTldkAzPCCqcoOsJGVEQTB;MT61{txO MmLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|OUi2OFgoRjF7M{m4OlQ5RC:jPh?=
HuH7 NVrmfo0zSW62aY\pdoFtKGG|c3H5 MnnXNlQhcHK| M1zWdGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJSlZiaX7m[YN1\WRiaX6gbJVu[W5iSIXIO{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC4aYLhcEB1emGwc3nlcpQhfHKjboPm[YN1cW:wIHHmeIVzKDJ2IHjyd{whTUN7ME2z{txO MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN7OE[0PEc,OTl|OUi2OFg9N2F-
HuH7 Ml\aRY51cX[rcnHsJIF{e2G7 NYXQdlNXOjRiaILz MnzJRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGijcnLvdolv\yCUTlGgdI9tgW2ncnHz[UBNOThyTTDteZRidnRiZ3Xu[UBqdm[nY4Tl[EBqdiCqdX3hckBJfUh5IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJJZqemGuIITyZY5{cWWwdDD0doFve2[nY4Tpc44h[W[2ZYKgNlQhcHK|LDDFR|UxRTF4zszN NX7wbIQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzPVg3PDhpPkG5N|k5PjR6PD;hQi=>
HuH7 MVXBcpRqfmm{YXygZZN{[Xl? NHzmTZczPCCqcoO= NHz2WnNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaHHyZo9zcW6pIGLORUBxd2y7bXXyZZNmKExzOEDNJI12fGGwdDDn[Y5mKGmwZnXjeIVlKGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygeJJidnOrZX70JJRz[W6|ZnXjeIlwdiCjZoTldkAzPCCqcoOsJGVEQTB;NEJOwG0> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN7OE[0PEc,OTl|OUi2OFg9N2F-
MT2 NIPZ[mxCdnSrdnnyZYwh[XO|YYm= Mk\aRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCrbn\lZ5Rm\CCrbjDoeY1idiCPVEKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBz\XCuaXPheIlwdixiSVO1NF0xNjcQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV7Nki4OUc,OTl3OU[4PFU9N2F-
HeLa P4/R5 M3XLNGFvfGm4aYLhcEBie3OjeR?= NV7IO2lCSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDpcoZm[3SnZDDpckBpfW2jbjDI[WxiKFB2L2K1JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25uIFnDOVA:OC55ON88US=> NUGxUoI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1PVY5QDVpPkG5OVk3QDh3PD;hQi=>
TZM-bl M{fFcmFvfGm4aYLhcEBie3OjeR?= MlrSO|IhcHK| MVjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHCQTEStN{Bp[XKkb4LpcochWmW4ZYLz[UB1emGwc3PybZB1[XOnIGCxNVlUKG23dHHueEBo\W6nIHnu[oVkfGWmIHnuJHRbVS2kbDDj[YxteyCjZoTldkA4OiCqcoOgZpkhdHWlaX\ldoF{\SCjc4PhfUwhTUN3ME2wMlQ4|ryP M2X2eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{C0NVMyLz5zOUewOFE{OTxxYU6=
TZM-bl NIPoNYVCdnSrdnnyZYwh[XO|YYm= M33vS2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRvdInw[UBJUVZzIIDOUFQuOyCrbn\lZ5Rm\CCrbjDUXm0u[mxiY3XscJMh[nlibIXjbYZmemG|ZTDhd5NigSxiRVO1NF0xNjZ3zszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTdyNEGzNUc,OTl5MESxN|E9N2F-
TZM-bl MojLRY51cX[rcnHsJIF{e2G7 NHeyOGg4OiCqcoO= MXfBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHCQTEStN{Bp[XKkb4LpcochWmW4ZYLz[UB1emGwc3PybZB1[XOnIGSxOlVCKG23dHHueEBo\W6nIHnu[oVkfGWmIHnuJHRbVS2kbDDj[YxteyCjZoTldkA4OiCqcoOgZpkhdHWlaX\ldoF{\SCjc4PhfUwhTUN3ME2xMlA5|ryP M1zCUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{C0NVMyLz5zOUewOFE{OTxxYU6=
TZM-bl M1m4W2FvfGm4aYLhcEBie3OjeR?= NHPqSZM4OiCqcoO= M37RWWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFicF7MOE0{KGijcnLvdolv\yCUZY\ldpNmKHS{YX7zZ5JqeHSjc3WgVFEyQVNxVEG2OWEhdXW2YX70JIdmdmViaX7m[YN1\WRiaX6gWHpONWKuIHPlcIx{KGGodHXyJFczKGi{czDifUBtfWOrZnXyZZNmKGG|c3H5MEBGSzVyPUKuNe69VQ>? M4PqZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{C0NVMyLz5zOUewOFE{OTxxYU6=
HepG2.2.15 NH75RZhCdnSrdnnyZYwh[XO|YYm= M3nHUGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDHNk4zNjF3IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygSG5CKHKncHzpZ4F1cW:wLDDJR|UxRTBwMkNOwG0> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh7N{O2N{c,OTl6OUezOlM9N2F-
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HepG22.2.15 NWq2dWFFSW62aY\pdoFtKGG|c3H5 MVOzJIRigXN? M2P0bmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDHNlIvOi5zNTDj[YxteyCjZoTldkA{KGSjeYOgZpkhdmW3dILhcEBz\WRiZInlMYJie2WmIIDsZZF2\SC{ZXT1Z5Rqd25iYYPzZZktKEmFNUC9NE4xOzoQvF2= NHHpTpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK2NFM{Pid-Mk[yOlA{OzZ:L3G+
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HepG2(2.2.15) NXr3OYZiSW62aY\pdoFtKGG|c3H5 NWPCXVZ3PiCmYYnz MWXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYh[Xm5MTDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOih{LkKuNVUqKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCC{ZYDsbYNifGmxbjDifUBu\WG|dYLpcochcW62cnHj[YxtfWyjcjD2bZJidCCGTlGgZ49xgSCwdX3i[ZIh[W[2ZYKgOkBl[Xm|IHL5JJJm[WxvdHnt[UByWEOUIH3leIhw\CxiRVO1NFwxNjBzzszN MlPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5NEi1PVAoRjJ5N{S4OVkxRC:jPh?=
HepG2(2.2.15) Mki1RY51cX[rcnHsJIF{e2G7 NWPLNXNrPiCmYYnz MoDGRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGJXKGG7d{GgbY5n\WO2ZXSgbY4hcHWvYX6gTIVxTzJqMj6yMlE2MSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxicnXwcIlk[XSrb36gZpkhdWWjc4XybY5oKGW6dILhZ4VtdHWuYYKgeolz[WxiRF7BJINweHliboXtZoVzKGGodHXyJFYh\GG7czDifUBz\WGuLYTpcYUheVCFUjDt[ZRpd2RuIFXDOVA:OC5yMd88US=> MorSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5NEi1PVAoRjJ5N{S4OVkxRC:jPh?=
MT4 MVjBcpRqfmm{YXygZZN{[Xl? MkjDOUBl[Xm| MYXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnu[oVkfGWmIHnuJIh2dWGwIF3UOEBk\WyuczDhd5Nme3OnZDDhd{Bxem:2ZXP0bY9vKGGpYXnud5Qhfmm{dYOtbY5lfWOnZDDjfZRweGG2aHnjJIVn\mWldDDh[pRmeiB3IHThfZMh[nliTWTUJIF{e2G7LDDFR|UxRTJwMkNOwG0> MonEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5NEi1PVAoRjJ5N{S4OVkxRC:jPh?=
MT4 MmW4RY51cX[rcnHsJIF{e2G7 MlHFOUBl[Xm| Mn;mRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOiCUT1SgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KHC{b4TlZ5Rqd25iYXfhbY5{fCC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIFnfGW{IEWg[IF6eyCkeTDNWHQh[XO|YYmsJGVEPTB;OD64Ne69VQ>? M4fENlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{S4OVkxLz5{N{e0PFU6ODxxYU6=
Huh7 NV\BSIRISW62aY\pdoFtKGG|c3H5 MlvhRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgcIFucX[3ZHnu[U1z\XOrc4ThcpQhf2muZDD0fZBmKGincHH0bZRqeyCEII\pdpV{KGmwZnXjeIVlKGmwIHj1cYFvKEi3aEegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKHOnY4LleIVlKESQQTDs[ZZmdHNibXXhd5Vz\WRib36g[IF6KDdiYomgdoVidC22aX3lJHBEWiCjbnHsfZNqeyxiRVO1NF0xNjB3Nd88US=> NE\MbVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4NlA3QCd-MkiwPFIxPjh:L3G+
HepG2.215 M{fBUWFvfGm4aYLhcEBie3OjeR?= NYfu[nNuSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhcGWyYYTpeIl{KEJidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hUGWyR{KuNlE2KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCCGTlGgdoVxdGmlYYTpc44hdWWjc4Xy[YQhd25iZHH5JFch[nlicnXhcEB1cW2nIGDDVkBidmGueYPpd{whTUN3ME2wMlHPxE1? M3Hw[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEiyNFY5Lz5{OEC4NlA3QDxxYU6=
Huh7 MofLRY51cX[rcnHsJIF{e2G7 MV3BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDsZY1qfnWmaX7lMZJme2m|dHHueEB4cWymIIT5dIUhcGWyYYTpeIl{KEJidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hUHWqNzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hcW62cnHj[YxtfWyjcjDEUmEhdGW4ZXzzJI1m[XO3cnXkJI9vKGSjeTC3JIJ6KHKnYXyteIlu\SCSQ2KgZY5idHm|aYOsJGVEPTB;MD6yN|fPxE1? NF\PdlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4NlA3QCd-MkiwPFIxPjh:L3G+
HepG2.215 NIfEe3JCdnSrdnnyZYwh[XO|YYm= NYHN[Yp2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhcGWyYYTpeIl{KEJidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hUGWyR{KuNlE2KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCCGTlGgdoVxdGmlYYTpc44hdWWjc4Xy[YQhd25iZHH5JFch[nlicnXhcEB1cW2nIGDDVkBidmGueYPpd{whTUN7ME2y{txO NXH3fJRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFIxPjhpPkK4NFgzODZ6PD;hQi=>
HepAD38 MYTBcpRqfmm{YXygZZN{[Xl? M1[xN|Uh\GG7cx?= NID1dnZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJSlZiaX7m[YN1\WRiaX6gbJVu[W5iSHXwRWQ{QCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGRPSSC{ZYDsbYNifGmxbjDh[pRmeiB3IHThfZMh[nliUmStVGNTKG2ndHjv[EwhTUN3ME2wMlA1|ryP M4LBOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEm0NVc6Lz5{OEC5OFE4QTxxYU6=
P4R5 MAGI M1LCNGZ2dmO2aX;uJIF{e2G7 MnP5NkBpenN? NHXkNYpKdmirYnn0bY9vKG:oIFjJWlEhSmGOIHTpcpVkdGWxc3nk[UBz\X[ncoPlJJRz[W6|Y4LpdJRie2ViaX7m[YN1\WRiaX6gbJVu[W5iUETSOUBOSUeLIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckB3cXKjbDDpcoZm[3Srb36gZYZ1\XJiMjDodpMh[nliR3HsZYN1dy2VdHHyJIJmfGFvZ3HsZYN1d3OrZHHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVEyNjQQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN3MUW4PEc,Ojh|NUG1PFg9N2F-
P4R5 MAGI MULGeY5kfGmxbjDhd5NigQ>? M13OSFIhcHK| MUPJcohq[mm2aX;uJI9nKEiLVkGgN2Ih\GmwdXPs[Y9{cWSnIILleoVze2VidILhcpNkemmydHHz[UBqdm[nY4Tl[EBqdiCqdX3hckBRPFJ3IF3BS2kh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKGmwZnXjeIlwdiCjZoTldkAzKGi{czDifUBI[WyjY4TvMXN1[XJiYnX0ZU1o[WyjY4Tvd4ll[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;M{KuO:69VQ>? NWPx[Yl[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkizOVE2QDhpPkK4N|UyPTh6PD;hQi=>
HepG2.2.15 M{f3ZmFvfGm4aYLhcEBie3OjeR?= MV[4JIRigXN? MXHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDI[ZBifGm2aYOgRkB3cXK3czDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOi5{LkG1JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhTE6DIILldIxq[2G2aX;uJIFnfGW{IEig[IF6eyCkeTDy[YFtKHSrbXWg[ox2d3Knc3PlcpQhWEOUIH3leIhw\CxiSVO1NF0xNjYQvF2= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7NEK1Nkc,Ojh2OUSyOVI9N2F-
MT4 MkLKRY51cX[rcnHsJIF{e2G7 NF\6c2w2KGSjeYO= MkDFRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCjZoTldkA2KGSjeYOgZpkhVVSWIHHzd4F6NCCHQ{WwQVIvPTUQvF2= M2TLclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW5NlQ3Lz5{OE[1PVI1PjxxYU6=
HepG2(2.2.15) NHvybJdCdnSrdnnyZYwh[XO|YYm= MUi2JIRigXN? MYPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYh[Xm5MTDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOih{LkKuNVUqKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCC{ZYDsbYNifGmxbjDifUBu\WG|dYLpcochcW62cnHj[YxtfWyjcjD2bZJidCCGTlGgZ49xgSCwdX3i[ZIh[W[2ZYKgOkBl[Xm|IHL5dkBjgSC{ZXHsMZRqdWVicWDDVkBie3OjeTygSWM2OD1yLkCwPFg4|ryP NIXj[W89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4NlA3Pyd-Mki2PFIxPjd:L3G+
HepG2(2.2.15) NXv2V5J4SW62aY\pdoFtKGG|c3H5 MYS2JIRigXN? MYLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIRnYh[Xm5MTDpcoZm[3SnZDDpckBpfW2jbjDI[ZBIOih{LkKuNVUqKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCC{ZYDsbYNifGmxbjDifUBu\WG|dYLpcoch\Xi2cnHj[YxtfWyjcjD2bZJidCCGTlGgZ49xgSCwdX3i[ZIh[W[2ZYKgOkBl[Xm|IHL5JJJm[WxvdHnt[UByWEOUIH3leIhw\CxiRVO1NF0xNjB{zszN MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ6MkC2O{c,Ojh4OEKwOlc9N2F-
HepAD38 NGPISFhCdnSrdnnyZYwh[XO|YYm= NUjMNXlsSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHnu[oVkfGWmIHnuJIh2dWGwIFjldGFFOzhiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIlvfHKjY3XscJVt[XJidnnyZYwhTE6DIHzleoVtKGK7IGLUMXBEWiCvZYToc4QtKEWFNUC8NE4xOc7:TR?= NWDqeVNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFgzQDBpPkK4Olg5OjhyPD;hQi=>
HepAD38 NH\3OnVCdnSrdnnyZYwh[XO|YYm= M1O5fmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDBSFM5KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCC{ZYDsbYNifGmxbjDifUBTXC2SQ2KgcYV1cG:mLDDFR|UxRTBwMEVOwG0> M3HE[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4NlgxLz5{OE[4PFI5ODxxYU6=
HepAD38 NFz1RWNCdnSrdnnyZYwh[XO|YYm= NXLkZVZUSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHnu[oVkfGWmIHnuJIh2dWGwIFjldGFFOzhiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIV5fHKjY3XscJVt[XJidnnyZYwhTE6DIHzleoVtKGK7IGLUMXBEWiCvZYToc4QtKEWFNUC9NE4xPM7:TR?= Mn\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4OEiyPFAoRjJ6Nki4NlgxRC:jPh?=
HepAD38 M3\kb2FvfGm4aYLhcEBie3OjeR?= M{D2SWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDBSFM5KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCC{ZYDsbYNifGmxbjDifUBTXC2SQ2KgcYV1cG:mLDDFR|kxRTBwM988US=> NFXHXmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PFI5OCd-Mki2PFgzQDB:L3G+
HepAD38 M2L6V2FvfGm4aYLhcEBie3OjeR?= NYntXGNKSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHnu[oVkfGWmIHnuJIh2dWGwIFjldGFFOzhiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIV5fHKjY3XscJVt[XJidnnyZYwhTE6DIHzleoVtKGK7IGLUMXBEWiCvZYToc4QtKEWFOUC9NE4{|ryP NYHHSXJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFgzQDBpPkK4Olg5OjhyPD;hQi=>
HepAD38 M{XpO2FvfGm4aYLhcEBie3OjeR?= NWXlT3c1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEKYIHnu[oVkfGWmIHnuJIh2dWGwIFjldGFFOzhiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIlvfHKjY3XscJVt[XJidnnyZYwhTE6DIHzleoVtKGK7IGLUMXBEWiCvZYToc4QtKEWFOUC9NE44|ryP M4\Be|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4NlgxLz5{OE[4PFI5ODxxYU6=
SupT1 M4nTOWFvfGm4aYLhcEBie3OjeR?= M37PU|Q5KGi{cx?= MmfERY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgWnNXNUdicIPleYRwfHmyZXSgTGlXOSCrbn\lZ5Rm\CCrbjDoeY1idiCVdYDUNUBk\WyuczDh[pRmeiB2ODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNe69VQ>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTBzNkGzNUc,OjlyMU[xN|E9N2F-
HepG2(2.2.15) M4fVbGFvfGm4aYLhcEBie3OjeR?= MYS2JIRigXN? M1iyRWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhDXiCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDHNkgzNjJwMUWpJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhTE6DIILldIxq[2G2aX;uJIFnfGW{IE[g[IF6eyCkeTDSWE1RS1JibXX0bI9lNCCLQ{WwQFAvOc7:TR?= NWfuZ|I1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyPFg6PDNpPkK5Nlg5QTR|PD;hQi=>
A549 M{j0emFvfGm4aYLhcEBie3OjeR?= NV\TbZdJSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTD2bZJqd26|IHTldol3\WRiZoLvcUBJUVZxVmPHMWchd3JiSFnWM2hCKH[rcnnvcpMhcW6oZXP0[YQhOjl|VDDj[YxteyC3c3nu[{BpfW2jbjDBOVQ6KGOnbHzzJIF{KHSjcnfleEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIIXzbY5oKGOxbYDveY5lKHC{ZT3tbZhm\CC4aYLpc45{KG[xbHzve4VlKGK7IHnuZ5Vj[XSrb36ge4l1cCC2YYLn[ZQh[2WuLDDJR|UxRTBwNUJOwG0> M3;hT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Nkm5PVI1Lz5{OU[5PVkzPDxxYU6=
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Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 229.26
Formula

C8H11N3O3S

CAS No. 134678-17-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(OC(S1)CO)N2C=CC(=NC2=O)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04133012 Recruiting Other: Samplings HIV-1 Infection ANRS Emerging Infectious Diseases|ViiV Healthcare February 10 2020 Not Applicable
NCT03869944 Recruiting Drug: Lamivudine Oral Solution HIV-1 ANRS Emerging Infectious Diseases|Institut National de la Santé Et de la Recherche Médicale France|Centre Muraz December 4 2019 Phase 2
NCT04034862 Not yet recruiting Drug: Treatment simplification (dolutegravir lamivudine) HIV Infections University of Liege October 1 2019 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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