Hydrocortisone

Catalog No.S1696

Hydrocortisone  Chemical Structure

Molecular Weight(MW): 362.46

Hydrocortisone is a steroid hormone or glucocorticoid produced by the adrenal gland.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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Choose Selective Glucocorticoid Receptor Inhibitors

Biological Activity

Description Hydrocortisone is a steroid hormone or glucocorticoid produced by the adrenal gland.
Targets
Glucocorticoid receptor [1]
()
In vitro

Hydrocortisone prevents endothelial barrier breakdown in response to pro-inflammatory stimuli (TNFalpha administration) in the human brain microvascular endothelial cell line hCMEC/D3, which could be demonstrated to be partly based on maintenance of occludin levels. [1] Hydrocortisone-treated dendritic cells (DCs) show a decreased expression of MHC II molecules, the costimulatory molecule CD86, and the DC-specific marker CD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibits production of IFN-gamma but induces an increased release of IL-4 and no change in IL-5. Hydrocortisone reduces T-cell proliferation in dendritic cells. [2] Hydrocortisone prevents TNF-alpha induced severe degradation of the glycocalyx, increased coronary resistance, heightened vascular leak and permeability to hydroxyethyl starch and caused mast-cell degranulation in isolated guinea pig hearts. [3] Hydrocortisone reduces postischemic oxidative stress, perfusion pressure, and transudate formation in isolated guinea pig hearts. Hydrocortisone inhibits postischemic shedding of syndecan-1, heparan sulfate, and hyaluronan, as well as release of histamine from resident mast cells. [4] Hydrocortisone increases the levels of IL-4-induced germ line C epsilon transcripts by twofold and delivers the signal required for transcription of mature C epsilon mRNA. Hydrocortisone induces S mu-S epsilon deletional switch recombination in IL-4-treated B cells, and support a model of sequential isotype switching from IgM to IgE via IgG4. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells MXPGeY5kfGmxbjDhd5NigQ>? M4LFfVI1KGh? M1OxSmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCLRl6t[4FudWFiaX7keYNm\CCQTzDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCkeTDHdolme3NicnXhZ5Rqd25uIFnDOVA:OC5yNkGg{txO NXrkTpRoOjF|NkGzN|g>
mouse L929 cells MofSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWnFbm56PiCmYYnz M2PXWmdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJGw6OjliY3XscJMh[W[2ZYKgOkBl[Xm| M3LqXVkzPjFzMx?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
parkin / AIMP2 ; 

PubMed: 28366931     


Western blot analysis of parkin and its toxic substrate AIMP2 in SH-SY5Y cells treated with hydrocortisone or DMSO control (left panel). β-actin was used as an internal loading control. Relative AIMP2 protein levels (right panel, n = 3) were normalized to that of β-actin. 

CREB; 

PubMed: 28366931     


Expression of parkin and CREB in SH-SY5Y cells in response to treatment of hydrocortisone in increasing dose determined by western blot.

28366931
Immunofluorescence
Glut2; 

PubMed: 29717618     


Double immunofluorescence staining showed that C-PEPTIDE expressing cells coexpressed GLUT2 after HC treatment of R1 mES-derived cells. Scale bar = 50 μm. HC, hydrocortisone.

29717618
Growth inhibition assay
Cell viability; 

PubMed: 22829975     


CLL cells were cultured alone with increasing concentrations of Hydrocortisone (f) for 1 day. Viable CLL cells were quantified by flow cytometry as percentage of Annexin V−/7-AAD− (lower left quadrant) after electronic gating on CD5+CD19+CD45+ CLL cells. The percentage of specific viable CLL cells was calculated. *P<0.05 was considered statistically significant. 

22829975

Protocol

Solubility (25°C)

In vitro DMSO 73 mg/mL (201.4 mM)
Ethanol 23 mg/mL (63.45 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.46
Formula

C21H30O5

CAS No. 50-23-7
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03688789 Not yet recruiting Other: Hydrocortisone supplementation Haematological Malignancy Rennes University Hospital January 1 2019 Not Applicable
NCT01422707 Withdrawn Drug: Hydrocortisone Hyperandrogenemia|Obesity|Polycystic Ovary Syndrome University of Virginia January 19 2018 Early Phase 1
NCT03282487 Enrolling by invitation Drug: Modified release hydrocortisone Adrenal Insufficiency The Adelaide and Meath Hospital incorporating The National Children''s Hospital September 5 2017 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID