Hydrocortisone (NSC 10483)

For research use only.

Catalog No.S1696 Synonyms: Cortisol

13 publications

Hydrocortisone (NSC 10483) Chemical Structure

CAS No. 50-23-7

Hydrocortisone (NSC 10483, Cortisol) is a steroid hormone or glucocorticoid produced by the adrenal gland.

Selleck's Hydrocortisone (NSC 10483) has been cited by 13 publications

Purity & Quality Control

Choose Selective Glucocorticoid Receptor Inhibitors

Biological Activity

Description Hydrocortisone (NSC 10483, Cortisol) is a steroid hormone or glucocorticoid produced by the adrenal gland.
Targets
Glucocorticoid receptor [1]
()
In vitro

Hydrocortisone prevents endothelial barrier breakdown in response to pro-inflammatory stimuli (TNFalpha administration) in the human brain microvascular endothelial cell line hCMEC/D3, which could be demonstrated to be partly based on maintenance of occludin levels. [1] Hydrocortisone-treated dendritic cells (DCs) show a decreased expression of MHC II molecules, the costimulatory molecule CD86, and the DC-specific marker CD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibits production of IFN-gamma but induces an increased release of IL-4 and no change in IL-5. Hydrocortisone reduces T-cell proliferation in dendritic cells. [2] Hydrocortisone prevents TNF-alpha induced severe degradation of the glycocalyx, increased coronary resistance, heightened vascular leak and permeability to hydroxyethyl starch and caused mast-cell degranulation in isolated guinea pig hearts. [3] Hydrocortisone reduces postischemic oxidative stress, perfusion pressure, and transudate formation in isolated guinea pig hearts. Hydrocortisone inhibits postischemic shedding of syndecan-1, heparan sulfate, and hyaluronan, as well as release of histamine from resident mast cells. [4] Hydrocortisone increases the levels of IL-4-induced germ line C epsilon transcripts by twofold and delivers the signal required for transcription of mature C epsilon mRNA. Hydrocortisone induces S mu-S epsilon deletional switch recombination in IL-4-treated B cells, and support a model of sequential isotype switching from IgM to IgE via IgG4. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NYeyV3pOTnWwY4Tpc44h[XO|YYm= M3nnTlI1KGh? Mmm5RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEmITj3nZY1u[SCrbnT1Z4VlKE6RIIDyc4R2[3Srb36gZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyC{ZXHjeIlwdixiSVO1NF0xNjB4MTFOwG0> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN4MUOzPEc,OjF|NkGzN|g9N2F-
mouse L929 cells NUPONGE1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NG[5R3k3KGSjeYO= NX\SUXk4T3Kxd4ToJIlvcGmkaYTpc44hd2ZibX;1d4UhVDl{OTDj[YxteyCjZoTldkA3KGSjeYO= NX\OdGcxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUK2NVE{Lz57Mk[xNVM9N2F-
RAW264.7 NF3rdJJCdnSraX7mcIFudWG2b4L5JIF{e2G7 M1rXR2FvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGK7IF3UWEBie3OjeTygTWM2OCB;IESwMlY1KM7:TT6= NEDKPHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3PVc1Oyd-MkC4O|k4PDN:L3G+
RAW264.7 M17GdmZ2dmO2aX;uJIF{e2G7 M1HtUVI1KGi{cx?= Mn7xTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bi\nSncjCyOEBpenNiYomgS5Jq\XO|IILlZYN1cW:wIHLhd4VlKHOyZXP0do9xcG:2b33leJJ6NCCLQ{WwJF0hPDBwNkSg{txONg>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyN{WxN{c,OjF6MEe1NVM9N2F-
RAW264.7 MWLGeY5kfGmxbjDhd5NigQ>? Ml3LNlQhcHK| M1nNWmlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bi\nSncjCyOEBpenNiYomgS5Jq\XO|IILlZYN1cW:wLDDJR|UxKD1iNECuOlQh|ryPLh?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4N{W1NEc,OjNyNke1OVA9N2F-
RAW264.7 MVLBcpRqcW6obHHtcYF1d3K7IHHzd4F6 M4\SclI1KGi{cx?= MWLBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDhd5NigSxiSVO1NEA:KDR|Lkig{txONg>? M3H0c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{W1PFUxLz5{M{e1OVg2ODxxYU6=
L929 MXLGeY5kfGmxbjDhd5NigQ>? M37ScFIxKGi{cx?= MUfEbZNxdGGlZX3lcpQhd2ZiMTD4JFExLy16IF2gc4YhYzFuMjyzMVNJZS22cnnhcYNqdm:ub37lJIFk\XSxbnnk[UBnem:vIHfseYNw[2:{dHnjc4llKHKnY3XweI9zKGmwIIPvcJVjdGViZoLhZ5Rqd25ib3[gcY92e2ViTEmyPUBk\WyuczDh[pRmeiB{MDDodpMtKEumIE2gNE4xPDNizszNMi=> M{i4RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl{NkGxN{c,QTJ4MUGzQE9iRg>?
HEK293 MoTvSpVv[3Srb36gZZN{[Xl? M2T0UGlvcGmkaYTpc44hd2ZiTWKtcYVlcWG2ZXSgeJJidnOjY4TpeoF1cW:wIH;mJIdidGGldH;zbYRie2VicnXwc5J1\XJiZ3Xu[UBqdiCKRVuyPVMh[2WubIOg[ZhxemW|c3nu[{AyOWKndHHIV2Qy M3LJelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{W5NFg5Lz5zNke1PVA5QDxxYU6=
RAW264.7 M4O4SmFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MXewMlUhfG9iNUCgeYcwdWx? NV;sNJE5PCCqcoO= Mln3RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IFzQV{1{fGmvdXzheIVlKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiSVytNVAheHKxZIXjeIlwdiCjdDCwMlUhfG9iNUCgeYcwdWxicILleJJm[XSnZDDmc5IhPCCqcoOg[o9tdG:5ZXSgZpkh[2ijbHzlcodmKHerdHigNUB2\y:vTDDMVHMh\m:{IES4JIhzeyCkeTDFUGlUSSC{ZXzheIl3\SC2bzDjc451em:u M17pUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{i0PFQ2Lz5{MUO4OFg1PTxxYU6=
RAW264.7 Ml7MRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? Ml;GNE42KHSxIEWwJJVoN22u M1KxTVQhcHK| MkfzRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IFzQV{1{fGmvdXzheIVlKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiSVytOkBxem:mdXP0bY9vKGG2IECuOUB1dyB3MDD1[{9udCCycnX0doVifGWmIH\vdkA1KGi{czDmc4xtd3enZDDifUBkcGGubHXu[4Uhf2m2aDCxJJVoN22OIFzQV{Bnd3JiNEigbJJ{KGK7IFXMTXNCKHKnbHH0bZZmKHSxIHPvcpRzd2x? NV\vcpNGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzPFQ5PDVpPkKxN|g1QDR3PD;hQi=>
RAW264.7 M2q4OGFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NU\0Xm1KOC53IITvJFUxKHWpL33s NWLjXYl2PCCqcoO= MmS3RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IFzQV{1{fGmvdXzheIVlKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiSVyxMYJmfGFicILv[JVkfGmxbjDheEAxNjVidH:gOVAhfWdxbXygdJJmfHKnYYTl[EBnd3JiNDDodpMh\m:ubH;3[YQh[nliY3jhcIxmdmenIIfpeIghOSC3Zz;tUEBNWFNiZn;yJFQ5KGi{czDifUBGVEmVQTDy[YxifGm4ZTD0c{Bkd262cn;s NH;1O5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO4OFg1PSd-MkGzPFQ5PDV:L3G+
RAW264.7 MUDBcpRqcW6obHHtcYF1d3K7IHHzd4F6 M2rScVAvPSC2bzC1NEB2\y:vbB?= NW\Ecmo4PCCqcoO= NYOxeZRLSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBi\2GrboP0JGxRWy2|dHnteYxifGWmIH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gWG5H[WyyaHGgdJJw\HWldHnvckBifCByLkWgeI8hPTBidXevcYwheHKndILlZZRm\CCob4KgOEBpenNiZn;scI94\WRiYomgZ4hidGynbnflJJdqfGhiMTD1[{9uVCCOUGOg[o9zKDR6IHjyd{BjgSCHTFnTRUBz\WyjdHn2[UB1dyClb370do9t NVHJ[m5ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzPFQ5PDVpPkKxN|g1QDR3PD;hQi=>
RAW264.7 MnPORY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NWO3SnVnOC53IITvJFUxKHWpL33s MXe0JIhzew>? NVvQW5NxSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBi\2GrboP0JGxRWy2|dHnteYxifGWmIH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25iYYSgNE42KHSxIEWwJJVoN22uIIDy[ZRz\WG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KGOqYXzs[Y5o\SC5aYToJFEhfWdxbVygUHBUKG[xcjC0PEBpenNiYomgS5Jq\XO|IILlZYN1cW:wIH3leIhw\CC{ZXzheIl3\SC2bzDjc451em:u MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN6NEi0OUc,OjF|OES4OFU9N2F-
neural precursor cells MoHaSpVv[3Srb36gZZN{[Xl? NHPDVYhKdmirYnn0bY9vKG:oIH7leZJwe3CqZYLlJJBzd2yrZnXyZZRqd25ib3[gcY92e2VibnX1doFtKHC{ZXP1dpNweiClZXzsd{BjgSCPVGSgZZN{[Xl? NVjQVHFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
RAW264.7 MnO4RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? MV6xNEB2VQ>? M{j2VFIhcHK| MUDBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiaX7jdoVie2ViaX6gWG5HNWGucHjhJIxmfmWuIHH0JFExKHWPIHnuZ5Vj[XSnZDDmc5IhOiCqcoOgdJJqd3JidH:gUHBUKGOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAzOiCqcoOgZpkhTUyLU1GgdoVt[XSrdnWgeI8h[2:wdILvcC=> NUXlW212RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NVU2PjFpPkK1OVE2PTZzPD;hQi=>
RAW264.7 M1LYOWFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NI\mfGUyOCC3TR?= NEW0[|UzKGi{cx?= NX65OHBwSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJIlv[3KnYYPlJIlvKEmOLU[gcIV3\WxiYYSgNVAhfU1iaX7jeYJifGWmIH\vdkAzKGi{czDwdolweiC2bzDMVHMh[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFIzKGi{czDifUBGVEmVQTDy[YxifGm4ZTD0c{Bkd262cn;s NX\UT|dZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NVU2PjFpPkK1OVE2PTZzPD;hQi=>
RAW264.7 NYTpZVNrSW62aXnu[oxidW2jdH;yfUBie3OjeR?= MYexNEB2VQ>? MUWyJIhzew>? M1H1ZWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDpcoNz\WG|ZTDpckBKVC1zYnX0ZUBt\X[nbDDheEAyOCC3TTDpcoN2[mG2ZXSg[o9zKDJiaILzJJBzcW:{IITvJGxRWyClaHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhOjJiaILzJIJ6KEWOSWPBJJJmdGG2aY\lJJRwKGOxboTyc4w> NX\0V4dKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NVU2PjFpPkK1OVE2PTZzPD;hQi=>
RAW264.7 MUjBcpRqcW6obHHtcYF1d3K7IHHzd4F6 MmDSNVAhfU1? MlrkNkBpenN? MX;BcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44h[XRiMUCgeW0hcW6ldXLheIVlKG[xcjCyJIhzeyCycnnvdkB1dyCOUGOgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEKyJIhzeyC{ZXzheIl3\SC2bzD2[Yhq[2ynLYTy[YF1\WRiY3;ueJJwdA>? M{PnbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUG1OVYyLz5{NUWxOVU3OTxxYU6=
B16F10 MmDFRZBweHSxc3nzJIF{e2G7 MUSyNEB2VQ>? NFHhd|Q{PiCqcoO= M4XlUGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gcY92e2ViQkG2SlExKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHPs[YF3\WRiY3HzdIF{\SB|IHzleoVtKGG2IEKwJJVOKGGodHXyJFM3KGi{czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN? M3nreVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nkm1O|MzLz5{Nk[5OVc{OjxxYU6=
B16F10 MV;BdI9xfG:|aYOgZZN{[Xl? NVTTNlc2OjBidV2= NUnybW1wOzZiaILz MkHaTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SCEMU\GNVAh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5icEWzJIV5eHKnc4Ppc44h[XRiMkCgeW0h[W[2ZYKgN|YhcHK|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnegZoF{\WRiRlHDV{BidmGueYPpdy=> NHXtUpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[5OVc{Oid-Mk[2PVU4OzJ:L3G+
B16F10 Mn7lRZBweHSxc3nzJIF{e2G7 MX:yNEB2VQ>? MlPzN|YhcHK| MWjJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIH3veZNmKEJzNl[xNEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[YQhSmG6IHX4dJJme3Orb36gZZQhOjBidV2gZYZ1\XJiM{[gbJJ{KGK7IIfld5Rmem5iYnzveEBidmGueYPpdy=> NWO5TY5MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2PVU4OzJpPkK2Olk2PzN{PD;hQi=>
B16F10 Mn75RY51cXS3bX;yJIFkfGm4aYT5JIF{e2G7 MXS2MlEyKG2pL3vn MX7BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCEMU\GNVAh[2WubIOgfIVvd2e{YX\0[YQhcW5iQ{W3RmwwUiCvb4Xz[UBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJR2dW:{IHfyc5d1cCCjdDC2MlEyKG2pL3vnMEBqeA>? MlHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4OUW3N|IoRjJ4Nkm1O|MzRC:jPh?=
B16F10 MoLkRZBweHSxc3nzJIF{e2G7 M{Wwb|IxKHWP M4L5S|M3KGi{cx?= MnHMTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SCEMU\GNVAh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iY4n0c4Npem:vZTDDJIxmfmWuIHH0JFIxKHWPIHHmeIVzKDN4IHjyd{BjgSC5ZYP0[ZJvKGKub4SgZY5idHm|aYO= M1;4NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nkm1O|MzLz5{Nk[5OVc{OjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
parkin / AIMP2 ; 

PubMed: 28366931     


Western blot analysis of parkin and its toxic substrate AIMP2 in SH-SY5Y cells treated with hydrocortisone or DMSO control (left panel). β-actin was used as an internal loading control. Relative AIMP2 protein levels (right panel, n = 3) were normalized to that of β-actin. 

CREB; 

PubMed: 28366931     


Expression of parkin and CREB in SH-SY5Y cells in response to treatment of hydrocortisone in increasing dose determined by western blot.

28366931
Immunofluorescence
Glut2; 

PubMed: 29717618     


Double immunofluorescence staining showed that C-PEPTIDE expressing cells coexpressed GLUT2 after HC treatment of R1 mES-derived cells. Scale bar = 50 μm. HC, hydrocortisone.

29717618
Growth inhibition assay
Cell viability; 

PubMed: 22829975     


CLL cells were cultured alone with increasing concentrations of Hydrocortisone (f) for 1 day. Viable CLL cells were quantified by flow cytometry as percentage of Annexin V−/7-AAD− (lower left quadrant) after electronic gating on CD5+CD19+CD45+ CLL cells. The percentage of specific viable CLL cells was calculated. *P<0.05 was considered statistically significant. 

22829975

Protocol

Solubility (25°C)

In vitro DMSO 73 mg/mL (201.4 mM)
Water Insoluble
Ethanol ''23 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.46
Formula

C21H30O5

CAS No. 50-23-7
Storage powder
in solvent
Synonyms Cortisol
Smiles CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O

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Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04700644 Completed -- Central Adrenal Insufficiency|Prader-Willi Syndrome Medical University of Warsaw May 15 2020 --
NCT04325490 Completed Drug: Liquid powder|Drug: Hydrocortisone cream 1% Intertrigo Mahidol University|HOE pharmaceuticals Sdn. Bhd. October 1 2019 Phase 4
NCT03688789 Not yet recruiting Other: Hydrocortisone supplementation Haematological Malignancy Rennes University Hospital January 1 2019 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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