Amphotericin B

For research use only.

Catalog No.S1636 Synonyms: NSC 527017

8 publications

Amphotericin B  Chemical Structure

CAS No. 1397-89-3

Amphotericin B (AMB, NSC 527017) is an amphipathic polyene antibiotic which permeabilizes ergosterol-containing membranes.

Selleck's Amphotericin B has been cited by 8 publications

Purity & Quality Control

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Biological Activity

Description Amphotericin B (AMB, NSC 527017) is an amphipathic polyene antibiotic which permeabilizes ergosterol-containing membranes.
ergosterol [1]
In vitro

Amphotericin B administration is limited by infusion-related toxicity, including fever and chills, an effect postulated to result from proinflammatory cytokine production by innate immune cells. Amphotericin B induces signal transduction and inflammatory cytokine release from cells expressing TLR2 and CD14. [1] Amphotericin B interacts with cholesterol, the major sterol of mammal membranes, thus limiting the usefulness of Amphotericin B due to its relatively high toxicity. Amphotericin B is dispersed as a pre-micellar or as a highly aggregated state in the subphase. [2] Amphotericin B only kills unicellular Leishmania promastigotes (LPs) when aqueous pores permeable to small cations and anions are formed. Amphotericin B (0.1 mM) induces a polarization potential, indicating K+ leakage in KCl-loaded liposomes suspended in an iso-osmotic sucrose solution. Amphotericin B (0.05 mM) exhibits a nearly total collapse of the negative membrane potential, indicating Na+ entry into the cells. [3]

In vivo Amphotericin B results in prolonging the incubation time and decreasing PrPSc accumulation in the hamster scrapie model. Amphotericin B markedly reduces PrPSc levels in mice with transmissible subacute spongiform encephalopathies (TSSE). [4] Amphotericin B exerts a direct effect on Plasmodium falciparum and influences eryptosis of infected erythrocytes, parasitemia and hostsurvival in murine malaria. Amphotericin B tends to delay the increase of parasitemia and significantly delays host death plasmodium berghei-infected mice. [5]


Solubility (25°C)

In vitro DMSO 22 mg/mL (23.8 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 924.08


CAS No. 1397-89-3
Storage powder
in solvent
Synonyms NSC 527017

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04993222 Completed Drug: Amphotericin B liposome for injection|Drug: AmBisome® Bioequivalence CSPC Zhongnuo Pharmaceutical (Shijiazhuang) Co. Ltd. June 4 2020 Phase 1
NCT03905447 Terminated Drug: PC945|Drug: Standard of Care Aspergillosis|Lung Transplant Infection Pulmocide Ltd September 17 2019 Phase 2
NCT03828773 Recruiting Drug: Posaconazole|Drug: Fluconazole Candidiasis|Fungal Infection|Acute Myeloid Leukemia|Genetic Predisposition|Aspergillosis Bochud Pierre-Yves|Swiss National Science Foundation|Centre Hospitalier Universitaire Vaudois February 11 2019 Not Applicable
NCT03814343 Recruiting Drug: amphotericin B in 30% DMSO|Drug: 30% DMSO Fungal Infection|Onychomycosis|Fungus Nail Mahidol University January 15 2019 Phase 4
NCT03399955 Recruiting Drug: Paromomycin|Drug: Ambisome|Drug: Miltefosine PKDL - Post-Kala-Azar Dermal Leishmanioid Drugs for Neglected Diseases May 9 2018 Phase 2
NCT02226705 Terminated Procedure: Multiple transnasal endoscopic surgeries Rhinocerebral Mucormycosis Assistance Publique - Hôpitaux de Paris January 2015 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID