Amphotericin B

For research use only.

Catalog No.S1636 Synonyms: NSC 527017

6 publications

Amphotericin B  Chemical Structure

Molecular Weight(MW): 924.08

Amphotericin B (AmB) is an amphipathic polyene antibiotic which permeabilizes ergosterol-containing membranes.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 130 In stock
USD 97 In stock
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Biological Activity

Description Amphotericin B (AmB) is an amphipathic polyene antibiotic which permeabilizes ergosterol-containing membranes.
Targets
ergosterol [1]
In vitro

Amphotericin B administration is limited by infusion-related toxicity, including fever and chills, an effect postulated to result from proinflammatory cytokine production by innate immune cells. Amphotericin B induces signal transduction and inflammatory cytokine release from cells expressing TLR2 and CD14. [1] Amphotericin B interacts with cholesterol, the major sterol of mammal membranes, thus limiting the usefulness of Amphotericin B due to its relatively high toxicity. Amphotericin B is dispersed as a pre-micellar or as a highly aggregated state in the subphase. [2] Amphotericin B only kills unicellular Leishmania promastigotes (LPs) when aqueous pores permeable to small cations and anions are formed. Amphotericin B (0.1 mM) induces a polarization potential, indicating K+ leakage in KCl-loaded liposomes suspended in an iso-osmotic sucrose solution. Amphotericin B (0.05 mM) exhibits a nearly total collapse of the negative membrane potential, indicating Na+ entry into the cells. [3]

In vivo Amphotericin B results in prolonging the incubation time and decreasing PrPSc accumulation in the hamster scrapie model. Amphotericin B markedly reduces PrPSc levels in mice with transmissible subacute spongiform encephalopathies (TSSE). [4] Amphotericin B exerts a direct effect on Plasmodium falciparum and influences eryptosis of infected erythrocytes, parasitemia and hostsurvival in murine malaria. Amphotericin B tends to delay the increase of parasitemia and significantly delays host death plasmodium berghei-infected mice. [5]

Protocol

Solubility (25°C)

In vitro DMSO 22 mg/mL (23.8 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 924.08
Formula

C47H73NO17

CAS No. 1397-89-3
Storage powder
in solvent
Synonyms NSC 527017
Smiles CC1OC(OC/2CC3OC(O)(CC(O)CC(O)C(O)CCC(O)CC(O)CC(=O)OC(C)C(C)C(O)C(C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C2)CC(O)C3C(O)=O)C(O)C(N)C1O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03905447 Recruiting Drug: PC945|Drug: Standard of Care Aspergillosis|Lung Transplant Infection Pulmocide Ltd September 17 2019 Phase 2
NCT03828773 Recruiting Drug: Posaconazole|Drug: Fluconazole Candidiasis|Fungal Infection|Acute Myeloid Leukemia|Genetic Predisposition|Aspergillosis Bochud Pierre-Yves|Swiss National Science Foundation|Centre Hospitalier Universitaire Vaudois February 11 2019 Not Applicable
NCT03814343 Recruiting Drug: amphotericin B in 30% DMSO|Drug: 30% DMSO Fungal Infection|Onychomycosis|Fungus Nail Mahidol University January 15 2019 Phase 4
NCT03399955 Recruiting Drug: Paromomycin|Drug: Ambisome|Drug: Miltefosine PKDL - Post-Kala-Azar Dermal Leishmanioid Drugs for Neglected Diseases May 9 2018 Phase 2
NCT02226705 Unknown status Procedure: Multiple transnasal endoscopic surgeries Rhinocerebral Mucormycosis Assistance Publique - Hôpitaux de Paris January 2015 Phase 2
NCT01845727 Completed Drug: Topical Amphotericin B at 3% Cutaneous Leishmaniasis Drugs for Neglected Diseases February 2014 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID