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XRCC1 Rabbit mAb

Catalog No.: F4109

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:100 IHC1:200 - 1:800 IF1:800 - 1:3200

Application
WB, IP, IHC, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
82 kDa

Datasheet & SDS

Biological Description

Specificity
XRCC1 Rabbit mAb detects endogenous levels of total XRCC1 protein.

Clone
H9H12

Synonym(s)
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; XRCC1

Background
XRCC1 (X-ray repair cross-complementing protein 1) is a pivotal scaffold protein involved in the DNA damage response, primarily coordinating the repair of single-strand breaks (SSBs) and facilitating base excision repair (BER). It is composed of three globular domains: the N-terminal domain binds DNA polymerase β, the central BRCT domain contains a poly(ADP-ribose) (PAR)-binding motif for PARP-1–dependent recruitment to DNA damage sites, and the C-terminal BRCT domain interacts with DNA ligase IIIα. These domains are connected by unstructured linker regions enriched with nuclear localization signals and motifs for post-translational modifications. XRCC1 orchestrates the spatial and temporal assembly of multiple DNA repair enzymes, including kinases, phosphatases, polymerases, deadenylases, and ligases, to accelerate the repair process. Post-translational modifications such as phosphorylation regulate XRCC1’s binding to partners like PNKP, APTX, and APLF, while ubiquitylation controls its protein turnover. Additionally, a redox-sensitive disulfide switch within the N-terminal domain modulates the binding affinity to polymerase β, thereby influencing the stability of the complex. XRCC1 also contributes to translesion synthesis via interaction with Rev1, supporting lesion bypass. It acts as a non-enzymatic assembly hub rather than a catalytic component. XRCC1 exhibits significant polymorphism, with variants like Arg194Trp and Arg399Gln influencing its interaction network, recruitment efficiency, DNA repair capability, and susceptibility to diseases, particularly various cancers.

Background

XRCC1 (X-ray repair cross-complementing protein 1) is a pivotal scaffold protein involved in the DNA damage response, primarily coordinating the repair of single-strand breaks (SSBs) and facilitating base excision repair (BER). It is composed of three globular domains: the N-terminal domain binds DNA polymerase β, the central BRCT domain contains a poly(ADP-ribose) (PAR)-binding motif for PARP-1–dependent recruitment to DNA damage sites, and the C-terminal BRCT domain interacts with DNA ligase IIIα. These domains are connected by unstructured linker regions enriched with nuclear localization signals and motifs for post-translational modifications. XRCC1 orchestrates the spatial and temporal assembly of multiple DNA repair enzymes, including kinases, phosphatases, polymerases, deadenylases, and ligases, to accelerate the repair process. Post-translational modifications such as phosphorylation regulate XRCC1’s binding to partners like PNKP, APTX, and APLF, while ubiquitylation controls its protein turnover. Additionally, a redox-sensitive disulfide switch within the N-terminal domain modulates the binding affinity to polymerase β, thereby influencing the stability of the complex. XRCC1 also contributes to translesion synthesis via interaction with Rev1, supporting lesion bypass. It acts as a non-enzymatic assembly hub rather than a catalytic component. XRCC1 exhibits significant polymorphism, with variants like Arg194Trp and Arg399Gln influencing its interaction network, recruitment efficiency, DNA repair capability, and susceptibility to diseases, particularly various cancers.

References
https://pubmed.ncbi.nlm.nih.gov/25795425/ https://pubmed.ncbi.nlm.nih.gov/31324530/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%