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Tin2 Rabbit mAb

Catalog No.: F3723

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
50 kDa 38 kDa, 39 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Tin2 Rabbit mAb detects endogenous levels of total Tin2 protein.

Clone
K6G20

Synonym(s)
TIN2, TINF2, TERF1-interacting nuclear factor 2, TRF1-interacting nuclear protein 2

Background
TIN2 (TRF1-interacting nuclear factor 2) is a central scaffold protein within the shelterin complex, a six-protein assembly that protects mammalian telomeres, regulating telomere length and integrity. TIN2 contains a telomere repeat factor homology (TRFH) domain, a TRFH-binding motif (TBM), and a domain associated with dyskeratosis congenita mutations. TIN2 interacts with TRF1 and TRF2, double-stranded telomeric DNA-binding proteins, through its TBM and TRFH domains, and directly binds TPP1 to facilitate the assembly of POT1 and TPP1 on telomeres, crucial for protecting single-stranded telomeric DNA. There are two major TIN2 isoforms, TIN2S and TIN2L, which modulate telomere stability and architecture. TIN2 coordinates cis (DNA compaction) and trans (DNA-DNA or DNA-RNA bridging) interactions, maintaining telomere structural integrity. Loss of TIN2 leads to telomere deprotection, activation of DNA damage responses (ATM/ATR kinases), chromatid fusion, and may result in embryonic lethality. TIN2 also interacts with the cohesin subunit SA1, regulating sister telomere cohesion, while Tankyrase1 controls TRF1-TIN2 association with telomeres. Acting as a cooperative platform, TIN2 bridges TRF1, TRF2, and TPP1, enabling shelterin functions in chromosome end protection, telomerase regulation, and genome stability. Mutations or dysfunction of TIN2 are linked to premature aging syndromes and cancer susceptibility.

Background

TIN2 (TRF1-interacting nuclear factor 2) is a central scaffold protein within the shelterin complex, a six-protein assembly that protects mammalian telomeres, regulating telomere length and integrity. TIN2 contains a telomere repeat factor homology (TRFH) domain, a TRFH-binding motif (TBM), and a domain associated with dyskeratosis congenita mutations. TIN2 interacts with TRF1 and TRF2, double-stranded telomeric DNA-binding proteins, through its TBM and TRFH domains, and directly binds TPP1 to facilitate the assembly of POT1 and TPP1 on telomeres, crucial for protecting single-stranded telomeric DNA. There are two major TIN2 isoforms, TIN2S and TIN2L, which modulate telomere stability and architecture. TIN2 coordinates cis (DNA compaction) and trans (DNA-DNA or DNA-RNA bridging) interactions, maintaining telomere structural integrity. Loss of TIN2 leads to telomere deprotection, activation of DNA damage responses (ATM/ATR kinases), chromatid fusion, and may result in embryonic lethality. TIN2 also interacts with the cohesin subunit SA1, regulating sister telomere cohesion, while Tankyrase1 controls TRF1-TIN2 association with telomeres. Acting as a cooperative platform, TIN2 bridges TRF1, TRF2, and TPP1, enabling shelterin functions in chromosome end protection, telomerase regulation, and genome stability. Mutations or dysfunction of TIN2 are linked to premature aging syndromes and cancer susceptibility.

References
https://pubmed.ncbi.nlm.nih.gov/39109533/ https://pubmed.ncbi.nlm.nih.gov/29160297/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%