TGF β Receptor II Mouse mAb

Catalog No.: F2400

Application: Reactivity: Human

Usage Information

Dilution
IHC1:50 - 1:200

Application
IHC, FCM

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
TGF β Receptor II Mouse mAb detects endogenous levels of total TGF-β Receptor II protein.

Clone
J15L22

Synonym(s)
TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TbetaR-II, TGFBR2

Background
The TGF β Receptor II, (transforming growth factor-β type II receptor, TβRII) is a type I transmembrane glycoprotein and a constitutively active serine/threonine kinase that initiates TGF-β signaling. It comprises an extracellular ligand-binding ectodomain with a three-finger toxin fold stabilized by six disulfide bonds (four conserved among type II receptors and two unique to TβRII), a single transmembrane segment, and an intracellular kinase domain. The ectodomain contains 12 cysteines and features an extended first “finger” compared to related receptors, enabling specific ligand interactions. TβRII is expressed in many tissues and binds TGF-β ligands, recruiting and phosphorylating the type I receptor (TβRI) to activate SMAD-dependent and SMAD-independent pathways, including Src kinase signaling. Through these mechanisms, TβRII regulates development, tissue homeostasis, immune responses, and pathological processes such as cancer and fibrosis.

Background

The TGF β Receptor II, (transforming growth factor-β type II receptor, TβRII) is a type I transmembrane glycoprotein and a constitutively active serine/threonine kinase that initiates TGF-β signaling. It comprises an extracellular ligand-binding ectodomain with a three-finger toxin fold stabilized by six disulfide bonds (four conserved among type II receptors and two unique to TβRII), a single transmembrane segment, and an intracellular kinase domain. The ectodomain contains 12 cysteines and features an extended first “finger” compared to related receptors, enabling specific ligand interactions. TβRII is expressed in many tissues and binds TGF-β ligands, recruiting and phosphorylating the type I receptor (TβRI) to activate SMAD-dependent and SMAD-independent pathways, including Src kinase signaling. Through these mechanisms, TβRII regulates development, tissue homeostasis, immune responses, and pathological processes such as cancer and fibrosis.

References
https://pubmed.ncbi.nlm.nih.gov/12121646/ https://pubmed.ncbi.nlm.nih.gov/36378749/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%