Survivin (C6B16) Rabbit mAb

Catalog No.: F2849

Usage Information

Dilution
WB1:5000-1:20000 IP1:150 IHC1:1000 IF1:100 - 1:250 FCM1:500

Application
WB, IP, IHC, IF, FCM, ELISA

Source
Rabbit

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
16 kDa

Datasheet & SDS

Biological Description

Specificity
Survivin (C6B16) Rabbit mAb detects endogenous levels of total Survivin protein.

Clone
C6B16

Background
Survivin, also known as BIRC5, is the smallest member of the inhibitor of apoptosis (IAP) protein family, characterized by a single N-terminal baculovirus IAP repeat (BIR) domain and a C-terminal coiled-coil region. It is an evolutionarily conserved protein essential for both cell division and inhibition of apoptosis. Uniquely expressed during embryonic development and in actively proliferating adult and cancer cells—but largely absent in normal, differentiated tissues—survivin plays a dual role in regulating cell proliferation and preventing cell death. It is structurally organized as a homodimer and forms critical interactions within the chromosomal passenger complex (CPC), essential for mitosis. Survivin undergoes various post-translational modifications (e.g., phosphorylation, acetylation, ubiquitination), influencing its stability, localization, and function. Despite possessing only one BIR domain and lacking direct caspase-binding ability, survivin cooperates with other IAPs (e.g., XIAP, c-IAP1/2) and associated proteins (e.g., HBXIP, XAF1) to suppress apoptosis and enhance cell survival. Its overexpression in numerous cancers correlates with poor prognosis, chemoresistance, and tumor aggressiveness, making survivin a promising biomarker and therapeutic target for cancer treatment.

Background

Survivin, also known as BIRC5, is the smallest member of the inhibitor of apoptosis (IAP) protein family, characterized by a single N-terminal baculovirus IAP repeat (BIR) domain and a C-terminal coiled-coil region. It is an evolutionarily conserved protein essential for both cell division and inhibition of apoptosis. Uniquely expressed during embryonic development and in actively proliferating adult and cancer cells—but largely absent in normal, differentiated tissues—survivin plays a dual role in regulating cell proliferation and preventing cell death. It is structurally organized as a homodimer and forms critical interactions within the chromosomal passenger complex (CPC), essential for mitosis. Survivin undergoes various post-translational modifications (e.g., phosphorylation, acetylation, ubiquitination), influencing its stability, localization, and function. Despite possessing only one BIR domain and lacking direct caspase-binding ability, survivin cooperates with other IAPs (e.g., XIAP, c-IAP1/2) and associated proteins (e.g., HBXIP, XAF1) to suppress apoptosis and enhance cell survival. Its overexpression in numerous cancers correlates with poor prognosis, chemoresistance, and tumor aggressiveness, making survivin a promising biomarker and therapeutic target for cancer treatment.

References
[1] Wheatley SP, et al. J Cell Sci. 2019 Apr 4;132(7):jcs223826. [2] Garg H, et al. Cancer Cell Int. 2016 Jun 23;16:49.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%