Sodium Nitrite

Catalog No.S4074

Sodium Nitrite Chemical Structure

Molecular Weight(MW): 69

Sodium nitrite is a myeloperoxidase inhibitor with IC50 of 1.3 μM.

Size Price Stock Quantity  
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Choose Selective ROS Inhibitors

Biological Activity

Description Sodium nitrite is a myeloperoxidase inhibitor with IC50 of 1.3 μM.
myeloperoxidase [1]
1.3 μM(Kd)
In vitro

Sodium nitrite is well known for its role in inhibiting the growth of Clostridium botulinum by inhibiting iron-sulfur clusters essential to energy metabolism. Sodium nitrite slows chlorination by univalently reducing myeloperoxidase to an inactive form and as a consequence is oxidized to nitrogen dioxide. Myeloperoxidase oxidizes free tyrosine to tyrosyl radicals that exchange with tyrosyl residues in peptides. These peptide radicals then couple with nitrogen dioxide to form 3-nitrotyrosyl residues. With neutrophils, myeloperoxidase-dependent nitration required a high concentration of nitrite (1 mM), is doubled by tyrosine, and increases 4-fold by superoxide dismutase. Superoxide is likely to inhibit nitration by reacting with nitrogen dioxide and/or tyrosyl radicals. [1] Sodium nitrite results in intoxication of white mice with decrease of red cell superoxide dismutase (SOD) and catalase activity. The total activity of glucose-6-phosphate dehydrogenase and dehydrogenase of 6-phosphogluconate as well as the activity of glutathione reductase are higher in the group of mice that receive sodium nitrite in comparison with the control group. [3]

In vivo Peak plasma levels of nitrite are achieved in both sexes approximately 30 min after oral exposure. The model predicts that 10% of the hemoglobin is oxidized to the ferric form after oral doses of 15.9 mg/kg in male rats and 11.0 mg/kg in female rats and after intravenous doses of 8.9 and 7.1 mg/kg in male and female rats, respectively. The t1/2 for recovery from methemoglobinemia is 60 to 120 min depending on dose and route of administration. Replacement of the Vmax of methemoglobin reductase with a value representative of humans predicted a 10% methemoglobinemia following an intravenous dose of 5.8 mg/kg, in close agreement with an observed value of 5.7 mg/kg for humans. [2]


Kinase Assay:


+ Expand

H2O2 uptake assay:

Reactions are started by adding 10 nM myeloperoxidase to 30 μM H 2 O 2 and varying concentrations of nitrite, in 100 mM phosphate buffer, pH 7.4, containing 20 μM DTPA. Steady state rates of H 2 O 2 loss at 21°C are calculated over the first minute. Data are means and ranges of at least duplicate experiments.
Cell Research:


+ Expand
  • Cell lines: Jurkat cells
  • Concentrations: ~25 μM
  • Incubation Time: 36 h
  • Method:

    cell density

    (Only for Reference)
Animal Research:


+ Expand
  • Animal Models: Twelve-week old Fischer 344 rats
  • Formulation: deionized water
  • Dosages: 20 mg/kg
  • Administration: tail vein injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (188.4 mM)
Water 13 mg/mL warmed (188.4 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% propylene glycol, 5% Tween 80, 65% D5W
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 69


CAS No. 7632-00-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02517697 Not yet recruiting Metabolic Syndrome|Hypertension Gladwin Mark MD|University of Pittsburgh March 2019 Phase 2
NCT03331146 Withdrawn Acute Kidney Injury University of Alabama at Birmingham October 1 2018 Phase 3
NCT03452917 Recruiting Out-Of-Hospital Cardiac Arrest University of Washington|National Heart Lung and Blood Institute (NHLBI) February 8 2018 Phase 2|Phase 3
NCT03015402 Recruiting Pulmonary Hypertension Secondary|Heart Failure Gladwin Mark MD|National Heart Lung and Blood Institute (NHLBI)|University of Pittsburgh October 30 2017 Phase 2
NCT02863068 Recruiting Sickle Cell Disease Montefiore Medical Center|Food and Drug Administration (FDA) July 1 2017 Phase 2
NCT02918552 Recruiting Heart Failure Gladwin Mark MD|National Institute on Aging (NIA)|University of Pittsburgh April 3 2017 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Sodium Nitrite | Sodium Nitrite supplier | purchase Sodium Nitrite | Sodium Nitrite cost | Sodium Nitrite manufacturer | order Sodium Nitrite | Sodium Nitrite distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID