Rad52 Mouse mAb

Catalog No.: F3135

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:100-1:1000 IHC1:50-1:500 IF1:50-1:500

Application
WB, IP, IHC, IF, ELISA

Reactivity
Human, Mouse, Rat

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
48 kDa

Datasheet & SDS

Biological Description

Specificity
Rad52 Mouse mAb detects endogenous levels of total Rad52 protein.

Clone
E10D20

Synonym(s)
DNA repair protein RAD52 homolog

Background
RAD52 is a human DNA/RNA-binding protein of 418 amino acids that forms multimeric, ring-shaped oligomers—historically described as heptamers with its highly conserved N-terminal domain (aa 1–208) mediating DNA binding and oligomerization, and its less conserved C-terminal domain containing binding sites for RAD51 and replication protein A (RPA). RAD52 is expressed in the nucleus of proliferating cells, where it plays multifaceted roles in DNA double-strand break repair pathways, most prominently in single-strand annealing (SSA) and as a backup factor for homologous recombination (HR) in BRCA-deficient contexts. In SSA, RAD52 anneals complementary single-stranded DNA overhangs and stimulates ERCC1–XPF nuclease activity, while in HR it can facilitate RAD51 loading, promote second-end capture, and maintain genomic stability. Although dispensable for viability in normal vertebrate cells, RAD52 becomes essential under specific DNA repair deficiencies, making it an attractive target for synthetic lethality-based cancer therapies.

Background

RAD52 is a human DNA/RNA-binding protein of 418 amino acids that forms multimeric, ring-shaped oligomers—historically described as heptamers with its highly conserved N-terminal domain (aa 1–208) mediating DNA binding and oligomerization, and its less conserved C-terminal domain containing binding sites for RAD51 and replication protein A (RPA). RAD52 is expressed in the nucleus of proliferating cells, where it plays multifaceted roles in DNA double-strand break repair pathways, most prominently in single-strand annealing (SSA) and as a backup factor for homologous recombination (HR) in BRCA-deficient contexts. In SSA, RAD52 anneals complementary single-stranded DNA overhangs and stimulates ERCC1–XPF nuclease activity, while in HR it can facilitate RAD51 loading, promote second-end capture, and maintain genomic stability. Although dispensable for viability in normal vertebrate cells, RAD52 becomes essential under specific DNA repair deficiencies, making it an attractive target for synthetic lethality-based cancer therapies.

References
https://pubmed.ncbi.nlm.nih.gov/36980703/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%