PSF1 Rabbit mAb

Catalog No.: F3447

    Application: Reactivity:

    Usage Information

    Dilution
    1:10000 - 1:50000
    1:100
    Application
    WB, IF, FCM
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    23 kDa 23 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.
    Positive Control Human fetal heart; Mouse heart; Rat heart; HeLa cell; HepG2 cell; Jurkat cell
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity

    PSF1 Rabbit mAb recognizes endogenous levels of total PSF1 protein.

    Uniprot ID
    Q14691
    Clone
    E5F3
    Synonym(s)
    KIAA0186, PSF1, GINS1, DNA replication complex GINS protein PSF1, GINS complex subunit 1
    Background

    Psf1 (Partner of Sld5-1), also referred to as KIAA0186 or GINS1, is a core component of the GINS complex involved in DNA replication. Structurally, Psf1 contains a primarily α-helical A-domain and a predominantly β-stranded B-domain. It plays a critical role in regulating the cell cycle, supporting DNA replication, and is essential for early embryonic development as well as tissue regeneration. The human Psf1 gene is located on chromosome 20p11.21 and shares approximately 88% sequence similarity with its mouse counterpart. Psf1 is notably active in maintaining cellular proliferation and promoting mitosis by modulating cell cycle progression. Its expression is tightly linked to highly proliferative states and has been associated with tumorigenesis due to its overexpression in actively dividing cells. In adult mice, high levels of Psf1 expression are found in tissues with abundant proliferative activity, such as the bone marrow, thymus, ovaries, and testes. In contrast, Psf1 is virtually undetectable in fully differentiated tissues like the liver, kidney, and brain, which contain few mitotically active cells. At the cellular level, Psf1 is present in dividing cells but absent in quiescent and terminally differentiated cells.

    References
    • https://pubmed.ncbi.nlm.nih.gov/36482653/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.