Phospho-Estrogen Receptor α (Ser118) Rabbit mAb

Catalog No.: F3358

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IF1:200

Application
WB, IF

Source
Rabbit

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
66 kDa 60 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	MCF7 cell (treated with b-Estradiol and EGF); MCF-7 cell (treated with epidermal growth factor)
Negative Control

Datasheet & SDS

Biological Description

Specificity
Phospho-Estrogen Receptor α (Ser118) Rabbit mAb detects endogenous levels of estrogen receptor α only when phosphorylated at serine 118.

Uniprot ID
P03372

Clone
L4E1

Synonym(s)
ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Background
Phospho-Estrogen Receptor α (Ser118) (ERα) is a ligand-activated nuclear hormone receptor that mediates estrogen signaling by regulating transcription of estrogen-responsive genes. Structurally, ERα comprises a modular architecture with an intrinsically disordered N-terminal domain (NTD) containing Ser118, a central DNA-binding domain, and a C-terminal ligand-binding domain. It is abundantly expressed in estrogen-responsive tissues, particularly in breast epithelium and many breast cancers. Phosphorylation at Ser118—triggered by estrogen (E2), growth factors (via MAPK), or selective modulators—induces conformational changes in the NTD that modulate protein-protein and DNA interactions. This site-specific phosphorylation is critical for gene-specific recruitment of ERα and transcriptional coregulators, thereby determining differential gene expression outcomes and highlighting its essential role in fine-tuning estrogen-mediated transcriptional responses.

Background

Phospho-Estrogen Receptor α (Ser118) (ERα) is a ligand-activated nuclear hormone receptor that mediates estrogen signaling by regulating transcription of estrogen-responsive genes. Structurally, ERα comprises a modular architecture with an intrinsically disordered N-terminal domain (NTD) containing Ser118, a central DNA-binding domain, and a C-terminal ligand-binding domain. It is abundantly expressed in estrogen-responsive tissues, particularly in breast epithelium and many breast cancers. Phosphorylation at Ser118—triggered by estrogen (E2), growth factors (via MAPK), or selective modulators—induces conformational changes in the NTD that modulate protein-protein and DNA interactions. This site-specific phosphorylation is critical for gene-specific recruitment of ERα and transcriptional coregulators, thereby determining differential gene expression outcomes and highlighting its essential role in fine-tuning estrogen-mediated transcriptional responses.

References
[1] Duplessis TT, et al. Endocrinology. 2011 Jun;152(6):2517-26.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%