Ouabain

Catalog No.S4016 Synonyms: NSC 25485

For research use only.

Ouabain (NSC 25485) is a selective Na+/K+, -ATPase inhibitor, binds to α2 /α3 subunit with Ki of 41 nM/15 nM.

Ouabain Chemical Structure

CAS No. 630-60-4

Selleck's Ouabain has been cited by 6 Publications

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Biological Activity

Description Ouabain (NSC 25485) is a selective Na+/K+, -ATPase inhibitor, binds to α2 /α3 subunit with Ki of 41 nM/15 nM.
Features A glycoside poison that binds to and inhibits the action of the Na+/K+ pump in the cell membrane.
Targets
α3 [1] α2 [1]
15 nM(Ki) 41 nM(Ki)
In vitro

Ouabain (100 nM) inhibits ATPase activities with 25%. [1] Ouabain (0.1 μM-1.0 μM) inhibits the Na+ pump and increases stored Ca2+ in cultured rat astrocytes. High Ouabain affinity isoforms (alpha2 in astrocytes, alpha3 in neurons and myocytes) are confined to a reticular distribution within the PM that paralleled underlying endoplasmic or sarcoplasmic reticulum. [2] Ouabain (0.5-1.0 mM) increases the levels of alpha1 and beta1 mRNAs, whereas it decreases those of alpha2 and beta2 mRNAs in cultured rat astrocytes. Ouabain increases alpha1 and beta1, but not alpha2 and beta2, proteins, and that the isoforms in control and ouabain-treated cultures. The ouabain-induced increase in alpha1 mRNA is blocked by cycloheximide (10 mM), the intracellular Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetraacetoxymethyl ester (30 mM), and FK506 (1 nM) in cultured rat astrocytes. [3] Ouabain (10 μM) induces significant PMD in both cell lines (28.1% of MDCK cells and 47.9% of Ma104 cells are gated in region M1, against 11.8% and 14.6% of the respective controls), but unexpectedly this effect is more remarkable in Ma104 cells. Ouabain (10 μM) induces a sustained increase in P-Tyr in MDCK cells and GSH almost completely reverted this effect, while the effect is not significant in Ma104 cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDCK Mnm4WHBgXFKDTmPQU3JVTVJ8IHPlcIwh[WOldX31cIF1cW:wIHnu NGP3fZJVWF:WUlHOV3BQWlSHUkqgZ4VtdCCjY3P1cZVt[XSrb36gbY4hV0GWUETDNU1mgHC{ZYPzbY5oKE2GQ1ugZ4VtdHNuIFvtQVAvOzkQvF2= M{fa[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2OUmzOlA1Lz5zNEm5N|YxPDxxYU6=
THP1 M2XkO2NmdGy3bHHyJJVxfGGtZTDpci=> MVSyOVAhdWdxbHn0[ZI> NYTT[Gp6OjRiaILz NXLBflZWS2WubIXsZZIhfXC2YXvlJIlvKG2ndHjpZ4ltdGmwLYPlcpNqfGm4ZTDTeIFxcHmub3PvZ4N2eyCjdYLleZMhSVSFQzCyOVkzOyCyaHHnc4N6fGm8ZXSgbY4hcHWvYX6gcYRzOSC|aWLORZMuKGmwZIXj[YQhVUSUMTDk[YZq[2mnboSgbJVu[W5iVFjQNUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBi[2O3bYXsZZRqd25iYYSgNlUxKG2pL3zpeIVzKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV2OES5N{c,OTd3NEi0PVM9N2F-
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MG 63 (6-TG R) NFu2fXhyUFSVIHHzd4F6 M3rUbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ|IDi2MXRIKFJrIHPlcIx{ NWrpdIpRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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In vivo

Ouabain (14.4 mg/kg.d s.c. intermittent) further increases total peripheral resistance (TPR) in rats with heart failure due to myocardial infarction (MI), while continuous Ouabain treatment normalized TPR in rats. Ouabain (14.4 mg/kg.d s.c. continuous) significantly improves basal and maximal CO (basal: 83 mL/min; maximal: 134 mL/min). [5]

Protocol (from reference)

Animal Research:

[5]

  • Animal Models: Male Wistar rats with heart failure due to myocardial infarction (MI)
  • Dosages: 14.4 mg/kg
  • Administration: Subcutaneous

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 728.77
Formula

C29H44O12.8H2O

CAS No. 630-60-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1C(C(C(C(O1)OC2CC(C3(C4C(CCC3(C2)O)C5(CCC(C5(CC4O)C)C6=CC(=O)OC6)O)CO)O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03034005 Unknown status Drug: Budesonide Asthma Bispebjerg Hospital March 27 2017 Phase 4
NCT04630132 Recruiting -- Aging|Frailty|Hypertension|CKD IRCCS San Raffaele|Cariplo Foundation March 25 2017 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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