OAC1

Catalog No.S7217 Synonyms: BAS 00287861

For research use only.

OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming. OAC1 can activate both Oct4 and Nanog promoter-driven luciferase reporter genes.

OAC1 Chemical Structure

CAS No. 300586-90-7

Selleck's OAC1 has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming. OAC1 can activate both Oct4 and Nanog promoter-driven luciferase reporter genes.
Targets
Oct4 [1]
In vitro

OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state. [1]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Human ESCs transfected with human Oct4 and Nanog luciferase reporter genes
  • Concentrations: ~1 μM
  • Incubation Time: 24 h
  • Method: The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.

Solubility (25°C)

In vitro

DMSO 47 mg/mL
(198.09 mM)
Ethanol 21 mg/mL
(88.51 mM)
Water Insoluble

Chemical Information

Molecular Weight 237.26
Formula

C14H11N3O

CAS No. 300586-90-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)C(=O)NC2=NC=C3C(=C2)C=CN3

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01161836 Completed Drug: Iniparib Advanced Solid Tumors Sanofi July 2010 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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