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OAC1 OCT activator

Name: OAC1
Brand: Selleck Chemicals
SKU: S7217
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S7217

OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming. This compound can activate both Oct4 and Nanog promoter-driven luciferase reporter genes.

Chemical Structure

Molecular Weight: 237.26

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S721701 DMSO]47 mg/mL]false]Ethanol]21 mg/mL]false]Water]Insoluble]false Purity: 99.21%

99.21

Related Targets	JAK STAT TGF-beta/Smad Wnt/beta-catenin ERK GSK-3 Casein Kinase Notch ROCK Hedgehog/Smoothened PKA Secretase PORCN KLF Stemness kinase SFRP Fascin
Related Products	O4I2

Chemical Information, Storage & Stability

Molecular Weight	237.26	Formula	C₁₄H₁₁N₃O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	300586-90-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	BAS 00287861			Smiles	C1=CC=C(C=C1)C(=O)NC2=NC=C3C(=C2)C=CN3

Solubility

In vitro
Batch:

DMSO : 47 mg/mL ( (198.09 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 21 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.35mg/ml (9.90mM)	Taking the 1 mL working solution as an example, add 50 μL of 47 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.235mg/ml (0.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 4.7 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Oct4 ^[1]
In vitro	OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, this compound enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using it along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. It seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. This chemical may be used to enhance the reprogramming of somatic cells to a pluripotent state. ^[1]
References	https://pubmed.ncbi.nlm.nih.gov/23213213/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01161836	Completed	Advanced Solid Tumors	Sanofi	July 2010	Phase 1

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