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OAC1

Name: OAC1
Brand: Selleck Chemicals
SKU: S7217
Rating: 5 (3 reviews)

Synonyms: BAS 00287861

Catalog No.S7217 Purity: 99.21%

OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming. OAC1 can activate both Oct4 and Nanog promoter-driven luciferase reporter genes.

OAC1 Chemical Structure

CAS: 300586-90-7

Selleck's OAC1 has been cited by 3 publications

Purity & Quality Control

Batch: S721701 DMSO] 47 mg/mL] false] Ethanol] 21 mg/mL] false] Water] Insoluble] false Purity: 99.21%

99.21

OAC1 Related Products

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Biological Activity

Description

OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming. OAC1 can activate both Oct4 and Nanog promoter-driven luciferase reporter genes.

Targets

Oct4 ^[1]

In vitro
In vitro	OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state. ^[1]
Cell Research	Cell lines	Human ESCs transfected with human Oct4 and Nanog luciferase reporter genes
	Concentrations	~1 μM
	Incubation Time	24 h
	Method	The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT01161836	Completed	Advanced Solid Tumors	Sanofi	July 2010	Phase 1

References

[1] Li W, et al. Proc Natl Acad Sci U S A, 2012, 109(51), 20853-20858.

Chemical Information & Solubility

Molecular Weight	237.26	Formula	C₁₄H₁₁N₃O
CAS No.	300586-90-7	SDF	Download OAC1 SDF
Smiles	C1=CC=C(C=C1)C(=O)NC2=NC=C3C(=C2)C=CN3
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 47 mg/mL ( (198.09 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 21 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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