MUC16 Rabbit mAb

Catalog No.: F3453

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IHC1:1000 - 1:10000

Application
WB, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
1519 kDa

Datasheet & SDS

Biological Description

Specificity
MUC16 Rabbit mAb detects endogenous levels of total MUC16 protein.

Clone
B21H3

Synonym(s)
CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125

Background
MUC16 (Mucin 16) is the largest known type I transmembrane mucin, originally identified as carbohydrate antigen 125 (CA125), a classic ovarian cancer biomarker. Encoded on chromosome 19p13.2 and comprising 22,152 amino acids, MUC16 has a massive molecular weight and consists of three main domains: an N-terminal extracellular region rich in O-glycosylation, a large tandem repeat domain containing 16 SEA (sea urchin sperm protein, enterokinase, agrin) modules, and a C-terminal domain with an extracellular segment, a transmembrane region, and a short cytoplasmic tail featuring an NLS, ERM-binding site, and phosphorylated tyrosine. Normally expressed in epithelial tissues, MUC16 is aberrantly overexpressed or mutated in various cancers (notably ovarian, pancreatic, and endometrial), where it promotes tumor progression via pathways such as PI3K/AKT, JAK2/STAT3, ERK/FBW7/c-Myc, and by enhancing cell adhesion, migration, glucose metabolism, and epithelial–mesenchymal transition. It also facilitates immune evasion by inhibiting NK and T cell activity, making it both a diagnostic marker and a promising therapeutic target.

Background

MUC16 (Mucin 16) is the largest known type I transmembrane mucin, originally identified as carbohydrate antigen 125 (CA125), a classic ovarian cancer biomarker. Encoded on chromosome 19p13.2 and comprising 22,152 amino acids, MUC16 has a massive molecular weight and consists of three main domains: an N-terminal extracellular region rich in O-glycosylation, a large tandem repeat domain containing 16 SEA (sea urchin sperm protein, enterokinase, agrin) modules, and a C-terminal domain with an extracellular segment, a transmembrane region, and a short cytoplasmic tail featuring an NLS, ERM-binding site, and phosphorylated tyrosine. Normally expressed in epithelial tissues, MUC16 is aberrantly overexpressed or mutated in various cancers (notably ovarian, pancreatic, and endometrial), where it promotes tumor progression via pathways such as PI3K/AKT, JAK2/STAT3, ERK/FBW7/c-Myc, and by enhancing cell adhesion, migration, glucose metabolism, and epithelial–mesenchymal transition. It also facilitates immune evasion by inhibiting NK and T cell activity, making it both a diagnostic marker and a promising therapeutic target.

References
https://pubmed.ncbi.nlm.nih.gov/38758220/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%