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Mox1 Mouse mAb

Catalog No.: F4321

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:100-1:1000 IP1:50-1:500 IF1:50-1:500

Application
WB, IP, IF, ELISA

Reactivity
Human, Mouse, Rat

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
65 kDa, 59 kDa, 22 kDa

Datasheet & SDS

Biological Description

Specificity
Mox1 Mouse mAb detects endogenous levels of total Mox1 protein.

Clone
M9G22

Synonym(s)
NADPH oxidase 1, NOX-1, Mitogenic oxidase 1 (MOX-1), NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, MOX1, NOH1.

Background
The Mox gene family, comprising Mox1 and Mox2, forms a distinct subgroup of non-clustered, divergent antennapedia-like homeobox genes expressed across various mesodermal tissues. Both Mox and Pax family genes are involved in overlapping developmental processes, exhibiting strong spatial and temporal co-expression during embryogenesis. Mox1 and Mox2 show high expression in developing somites, particularly within the sclerotome, where they are co-expressed with Pax1. Additionally, Mox2 and Pax3 are both present in migrating myoblasts. Functionally, Mox1 is essential for axial skeleton formation, while Mox2 plays a key role in limb muscle development. Beyond development, Mox1 is involved in the regulation of reactive oxygen species (ROS), particularly in non-phagocytic cells, and shares structural similarity with the glycoprotein gp91^phox, a core subunit of the NADPH oxidase complex. This complex generates superoxide, a reactive molecule central to numerous signaling pathways and immune responses. Mox1 expression is most abundant in the colon, with lower levels in the uterus and prostate, and is upregulated in vascular smooth muscle cells in response to platelet-derived growth factor stimulation. Mox1 contributes to the modulation of NADPH oxidase activity in tissues where ROS production is critical for signaling and adaptation to hypoxic conditions. The distinct tissue distribution of Mox1 compared to gp91phox further points to potential functional divergence, with Mox1 acting as a specialized regulator of oxidative stress responses.

Background

The Mox gene family, comprising Mox1 and Mox2, forms a distinct subgroup of non-clustered, divergent antennapedia-like homeobox genes expressed across various mesodermal tissues. Both Mox and Pax family genes are involved in overlapping developmental processes, exhibiting strong spatial and temporal co-expression during embryogenesis. Mox1 and Mox2 show high expression in developing somites, particularly within the sclerotome, where they are co-expressed with Pax1. Additionally, Mox2 and Pax3 are both present in migrating myoblasts. Functionally, Mox1 is essential for axial skeleton formation, while Mox2 plays a key role in limb muscle development. Beyond development, Mox1 is involved in the regulation of reactive oxygen species (ROS), particularly in non-phagocytic cells, and shares structural similarity with the glycoprotein gp91^phox, a core subunit of the NADPH oxidase complex. This complex generates superoxide, a reactive molecule central to numerous signaling pathways and immune responses. Mox1 expression is most abundant in the colon, with lower levels in the uterus and prostate, and is upregulated in vascular smooth muscle cells in response to platelet-derived growth factor stimulation. Mox1 contributes to the modulation of NADPH oxidase activity in tissues where ROS production is critical for signaling and adaptation to hypoxic conditions. The distinct tissue distribution of Mox1 compared to gp91phox further points to potential functional divergence, with Mox1 acting as a specialized regulator of oxidative stress responses.

References
https://pubmed.ncbi.nlm.nih.gov/1363541/#&dopt=Abstract https://pubmed.ncbi.nlm.nih.gov/11423130/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%