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Monocyte + Macrophage Rat mAb

Catalog No.: F3741

Application: Reactivity: Mouse

Usage Information

Dilution

Application
IHC, FCM

Reactivity
Mouse

Source
Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
Monocyte + Macrophage Rat mAb detects endogenous levels of total Monocyte + Macrophage antigen. This antibody recognises an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs in all mouse strains.

Clone
E17C4

Background
Monocytes and macrophages are essential components of the innate immune system, playing a central role in initiating and coordinating inflammatory responses. Beyond driving the production of inflammatory mediators and shaping both innate and adaptive immunity, they are equally critical in resolving inflammation and restoring tissue homeostasis. Dysregulation of their function is therefore a common feature in the pathogenesis of chronic infections, autoimmune conditions, and severe sterile inflammatory diseases. Monocytes, which represent 5–10% of circulating leukocytes, are bone marrow–derived mononuclear cells with a short life span of 1–3 days. Under steady-state conditions, they support homeostasis and retain the capacity to differentiate into tissue macrophages. During inflammation, monocytes are actively recruited to affected sites, where they differentiate into inflammatory macrophages or dendritic cells. Macrophages are distributed throughout all tissues of the body and display remarkable functional heterogeneity. They are indispensable for tissue development, immune surveillance, and the maintenance of local homeostasis. Many macrophage populations are established during embryogenesis, arising from yolk sac or fetal liver progenitors, and can persist independently of monocyte replenishment under normal conditions. By contrast, macrophages in tissues such as the skin, heart, and intestine are initially seeded by embryonic progenitors but are rapidly replaced after birth by monocytes derived from hematopoietic stem cells. Importantly, tissue-resident macrophages possess both proliferative capacity and self-renewal potential. The activation and polarization of both monocytes and macrophages are triggered by their recognition of pathogen-associated or damage-associated molecular patterns (PAMPs and DAMPs) via pattern recognition receptors (PRRs), enabling them to adapt their responses to a wide variety of physiological and pathological signals.

Background

Monocytes and macrophages are essential components of the innate immune system, playing a central role in initiating and coordinating inflammatory responses. Beyond driving the production of inflammatory mediators and shaping both innate and adaptive immunity, they are equally critical in resolving inflammation and restoring tissue homeostasis. Dysregulation of their function is therefore a common feature in the pathogenesis of chronic infections, autoimmune conditions, and severe sterile inflammatory diseases. Monocytes, which represent 5–10% of circulating leukocytes, are bone marrow–derived mononuclear cells with a short life span of 1–3 days. Under steady-state conditions, they support homeostasis and retain the capacity to differentiate into tissue macrophages. During inflammation, monocytes are actively recruited to affected sites, where they differentiate into inflammatory macrophages or dendritic cells. Macrophages are distributed throughout all tissues of the body and display remarkable functional heterogeneity. They are indispensable for tissue development, immune surveillance, and the maintenance of local homeostasis. Many macrophage populations are established during embryogenesis, arising from yolk sac or fetal liver progenitors, and can persist independently of monocyte replenishment under normal conditions. By contrast, macrophages in tissues such as the skin, heart, and intestine are initially seeded by embryonic progenitors but are rapidly replaced after birth by monocytes derived from hematopoietic stem cells. Importantly, tissue-resident macrophages possess both proliferative capacity and self-renewal potential. The activation and polarization of both monocytes and macrophages are triggered by their recognition of pathogen-associated or damage-associated molecular patterns (PAMPs and DAMPs) via pattern recognition receptors (PRRs), enabling them to adapt their responses to a wide variety of physiological and pathological signals.

References
https://pubmed.ncbi.nlm.nih.gov/35741108/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%