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Mono Methyl-Histone H4 (Lys20) Rabbit mAb

Catalog No.: F3868

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:5000 IHC1:100 IF1:100 CHIP4 µg for 7 µg chromatin

Application
WB, IHC, IF, ChIP

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
11 kDa 11 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Mono Methyl-Histone H4 (Lys20) Rabbit mAb detects endogenous levels of total Mono Methyl-Histone H4 (Lys20) protein.

Clone
P14F3

Synonym(s)
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H

Background
The nucleosome, composed of the four core histone proteins H2A, H2B, H3, and H4, represents the fundamental structural unit of chromatin. While once regarded as a passive scaffold for DNA packaging, histones are now recognized as highly dynamic proteins that undergo a wide variety of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation plays a central role in defining transcriptionally active and inactive chromatin regions and is essential for proper genome programming during development. Lysine residues are the primary targets of histone methylation, occurring most prominently on histone H3 (at Lys4, Lys9, Lys27, Lys36, and Lys79) and histone H4 (at Lys20). These modifications can function in either transcriptional activation or repression, depending on their position and context. Methylated lysines act as docking sites for chromatin-associated proteins that contain specialized methyl-lysine recognition modules, including chromodomains (e.g., HP1, PRC1), PHD fingers (e.g., BPTF, ING2), tudor domains (e.g., 53BP1), and WD40 repeats (e.g., WDR5). Through these interactions, histone methylation coordinates the recruitment of chromatin-modifying complexes and thereby regulates genome organization and gene expression.

Background

The nucleosome, composed of the four core histone proteins H2A, H2B, H3, and H4, represents the fundamental structural unit of chromatin. While once regarded as a passive scaffold for DNA packaging, histones are now recognized as highly dynamic proteins that undergo a wide variety of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation plays a central role in defining transcriptionally active and inactive chromatin regions and is essential for proper genome programming during development. Lysine residues are the primary targets of histone methylation, occurring most prominently on histone H3 (at Lys4, Lys9, Lys27, Lys36, and Lys79) and histone H4 (at Lys20). These modifications can function in either transcriptional activation or repression, depending on their position and context. Methylated lysines act as docking sites for chromatin-associated proteins that contain specialized methyl-lysine recognition modules, including chromodomains (e.g., HP1, PRC1), PHD fingers (e.g., BPTF, ING2), tudor domains (e.g., 53BP1), and WD40 repeats (e.g., WDR5). Through these interactions, histone methylation coordinates the recruitment of chromatin-modifying complexes and thereby regulates genome organization and gene expression.

References
https://pubmed.ncbi.nlm.nih.gov/15268870/ https://pubmed.ncbi.nlm.nih.gov/15479858/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%