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MCU (A6E7) Rabbit mAb

Catalog No.: F3883

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:500 FCM1:500

Application
WB, IP, IHC, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
40 kDa 30 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
MCU (A6E7) Rabbit mAb detects endogenous levels of total MCU protein.

Clone
A6E7

Synonym(s)
C10orf42, CCDC109A, MCU, HsMCU, Coiled-coil domain-containing protein 109A

Background
The Mitochondrial Calcium Uniporter (MCU), encoded by the CCDC109A gene, is the pore-forming subunit of a vital calcium channel located in the inner mitochondrial membrane. It facilitates the selective uptake of Ca²⁺ ions into the mitochondrial matrix, playing a central role in calcium homeostasis, cellular metabolism, energy production, and apoptotic signaling. MCU contains two transmembrane helices connected by a loop featuring a conserved DIME motif critical for Ca²⁺ selectivity, along with regulatory N-terminal and C-terminal domains. Functioning as a tetramer, each MCU subunit helps form a selective filter that allows single Ca²⁺ ions to pass, driven by the mitochondrial membrane potential. MCU activity is precisely regulated by MICU1, MICU2, and EMRE, which together form the mitochondrial calcium uniporter complex (uniplex). MICU1 and MICU2 act as calcium-sensitive gatekeepers, preventing calcium overload at rest and activating uptake when cytosolic Ca²⁺ rises, while EMRE stabilizes the complex and facilitates inter-subunit communication. This tight regulation supports aerobic metabolism, ROS modulation, and the coordination of cell death pathways. Abnormal MCU function or regulation is linked to diseases such as neurodegeneration, cancer, and ischemia-reperfusion injury.

Background

The Mitochondrial Calcium Uniporter (MCU), encoded by the CCDC109A gene, is the pore-forming subunit of a vital calcium channel located in the inner mitochondrial membrane. It facilitates the selective uptake of Ca²⁺ ions into the mitochondrial matrix, playing a central role in calcium homeostasis, cellular metabolism, energy production, and apoptotic signaling. MCU contains two transmembrane helices connected by a loop featuring a conserved DIME motif critical for Ca²⁺ selectivity, along with regulatory N-terminal and C-terminal domains. Functioning as a tetramer, each MCU subunit helps form a selective filter that allows single Ca²⁺ ions to pass, driven by the mitochondrial membrane potential. MCU activity is precisely regulated by MICU1, MICU2, and EMRE, which together form the mitochondrial calcium uniporter complex (uniplex). MICU1 and MICU2 act as calcium-sensitive gatekeepers, preventing calcium overload at rest and activating uptake when cytosolic Ca²⁺ rises, while EMRE stabilizes the complex and facilitates inter-subunit communication. This tight regulation supports aerobic metabolism, ROS modulation, and the coordination of cell death pathways. Abnormal MCU function or regulation is linked to diseases such as neurodegeneration, cancer, and ischemia-reperfusion injury.

References
https://pubmed.ncbi.nlm.nih.gov/28194521/ https://pubmed.ncbi.nlm.nih.gov/28740830/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%