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Histone H2B Mouse mAb

Catalog No.: F3792

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:20000-1:50000 IF1:2000 FCM1:100 CHIPUse 2 µg for 25 µg of chromatin.

Application
WB, IP, IHC, IF, FCM, ChIP

Reactivity
Mouse, Rat, Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
15 kDa 15 kDa, 17 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Histone H2B Mouse mAb detects endogenous levels of total Histone H2B protein.

Clone
P21H8

Synonym(s)
H2BFF, HIST1H2BB, H2BC3, Histone H2B type 1-B, H2B-clustered histone 3, Histone H2B.1, Histone H2B.f, H2B/f

Background
Histone H2B is one of the five major histone proteins, along with H1/H5, H2A, H3, and H4. Within the nucleosome—the fundamental structural unit of chromatin—two molecules each of H2A, H2B, H3, and H4 form an octameric core around which DNA wraps in a left-handed superhelix. Histones are subject to numerous post-translational modifications (PTMs) that are critical for regulating transcription, DNA replication and repair, and maintaining chromosome stability. While H2A and H3 isoforms fulfill essential roles in chromatin regulation, H2B also has distinct, specialized functions. In humans, there are 16 known H2B isoforms—13 expressed in somatic cells and 3 restricted to the testes. The somatic isoforms are encoded by genes located in histone cluster 1 on chromosome 6 (6p21–6p22) and in clusters 2 (1q21) and 3 (1q42) on chromosome 1. These genes produce mRNA containing characteristic 3′ stem-loop structures and histone downstream elements, features that promote transcript stability during S-phase, when histone production sharply increases. Histone H2B is also involved in gene transcription regulation and can function outside the nucleus as a receptor and antimicrobial agent. Its N-terminal tail, rich in lysine and arginine residues, is a hotspot for epigenetic modifications—including ubiquitination, phosphorylation, methylation, and acetylation—which influence chromatin dynamics and transcriptional activity. Under stimulation by pathogens such as bacteria, viruses, or parasites, H2B can act as a recognition receptor, triggering intrinsic immune responses that strengthen host defenses. Extrachromosomal H2B also exhibits potent antimicrobial activity, making it a potential target for the development of future antibiotic strategies.

Background

Histone H2B is one of the five major histone proteins, along with H1/H5, H2A, H3, and H4. Within the nucleosome—the fundamental structural unit of chromatin—two molecules each of H2A, H2B, H3, and H4 form an octameric core around which DNA wraps in a left-handed superhelix. Histones are subject to numerous post-translational modifications (PTMs) that are critical for regulating transcription, DNA replication and repair, and maintaining chromosome stability. While H2A and H3 isoforms fulfill essential roles in chromatin regulation, H2B also has distinct, specialized functions. In humans, there are 16 known H2B isoforms—13 expressed in somatic cells and 3 restricted to the testes. The somatic isoforms are encoded by genes located in histone cluster 1 on chromosome 6 (6p21–6p22) and in clusters 2 (1q21) and 3 (1q42) on chromosome 1. These genes produce mRNA containing characteristic 3′ stem-loop structures and histone downstream elements, features that promote transcript stability during S-phase, when histone production sharply increases. Histone H2B is also involved in gene transcription regulation and can function outside the nucleus as a receptor and antimicrobial agent. Its N-terminal tail, rich in lysine and arginine residues, is a hotspot for epigenetic modifications—including ubiquitination, phosphorylation, methylation, and acetylation—which influence chromatin dynamics and transcriptional activity. Under stimulation by pathogens such as bacteria, viruses, or parasites, H2B can act as a recognition receptor, triggering intrinsic immune responses that strengthen host defenses. Extrachromosomal H2B also exhibits potent antimicrobial activity, making it a potential target for the development of future antibiotic strategies.

References
https://pubmed.ncbi.nlm.nih.gov/25922622/ https://pubmed.ncbi.nlm.nih.gov/38657835/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%