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Histone H2A.Z Rabbit mAb

Catalog No.: F3691

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:2000 IF1:100 - 1:250 FCM1:2500

Application
WB, IHC, IF, FCM, ChIP

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
13 kDa 13 kDa, 15 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Histone H2A.Z Rabbit mAb detects endogenous levels of total Histone H2A.Z protein.

Clone
A14P4

Synonym(s)
H2AFZ, H2AZ, H2AZ1, Histone H2A.Z, H2A/z

Background
Histone H2A.Z is a conserved variant of the canonical H2A histone found in most eukaryotes and plays a key role in chromatin organization and epigenetic regulation. It replaces H2A in about 5–10% of nucleosomes and is structurally distinct due to its extended L1 loop and unique C-terminal docking domain. These differences enhance histone-histone interactions and influence nucleosome stability and flexibility. Nucleosomes containing H2A.Z wrap approximately 11 base pairs less DNA on one side, increasing DNA accessibility and nucleosome mobility. The flexible DNA ends and altered docking domain enable differential interactions with linker histones and chromatin-associated proteins such as HP1, contributing to changes in chromatin compaction and structure. H2A.Z’s distinctive acidic patch and tails support both gene repression and activation, depending on context. It is involved in transcription regulation, DNA repair, maintenance of chromatin boundaries, and chromosome segregation. H2A.Z is deposited by the SWR chromatin remodeling complex, and its precise incorporation is essential for genome stability. Dysregulation of H2A.Z has been linked to cancer and developmental disorders due to its impact on gene expression and chromatin dynamics.

Background

Histone H2A.Z is a conserved variant of the canonical H2A histone found in most eukaryotes and plays a key role in chromatin organization and epigenetic regulation. It replaces H2A in about 5–10% of nucleosomes and is structurally distinct due to its extended L1 loop and unique C-terminal docking domain. These differences enhance histone-histone interactions and influence nucleosome stability and flexibility. Nucleosomes containing H2A.Z wrap approximately 11 base pairs less DNA on one side, increasing DNA accessibility and nucleosome mobility. The flexible DNA ends and altered docking domain enable differential interactions with linker histones and chromatin-associated proteins such as HP1, contributing to changes in chromatin compaction and structure. H2A.Z’s distinctive acidic patch and tails support both gene repression and activation, depending on context. It is involved in transcription regulation, DNA repair, maintenance of chromatin boundaries, and chromosome segregation. H2A.Z is deposited by the SWR chromatin remodeling complex, and its precise incorporation is essential for genome stability. Dysregulation of H2A.Z has been linked to cancer and developmental disorders due to its impact on gene expression and chromatin dynamics.

References
https://pubmed.ncbi.nlm.nih.gov/34643712/ https://pubmed.ncbi.nlm.nih.gov/36765119/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%