Choose Your Country or Region

HA-Tag Mouse mAb

Catalog No.: F5084

1/

Cited by 1 publication

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP, Elisa

Source
Mouse

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃

Storage (from the date of receipt)

Datasheet & SDS

Biological Description

Specificity
HA-Tag Mouse mAb detects exogenously expressed proteins containing the HA epitope tag. The antibody may cross-react with a protein of unknown origin ~100kDa.

Synonym(s)
Hemagglutinin Tag

Clone
G15H8

Background

Epitope tags are widely applied in protein labeling and detection via techniques such as immunoblotting, immunoprecipitation, and immunostaining. Due to their small molecular size, they typically have minimal impact on the biochemical characteristics of the target protein. The HA tag, originally derived from a segment of the influenza virus hemagglutinin protein, has become a commonly used tag in various expression systems.

References
[1] Field, J. et al. (1988) Mol Cell Biol 8, 2159-65

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field