Choose Your Country or Region

Glut4 Mouse mAb

Catalog No.: F0174

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse, Rat

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50 kDa

Datasheet & SDS

Biological Description

Specificity
Glut4 Mouse mAb recognizes endogenous levels of total Glut4 protein.

Clone
G3B7

Synonym(s)
Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, SLC2A4, GLUT4

Background
GLUT4 (Glucose Transporter Type 4) is a member of the SLC2 family of facilitative glucose transporters, primarily found in insulin-sensitive tissues such as skeletal muscle, adipose tissue, and the heart. It is essential for the regulation of glucose homeostasis, as it facilitates glucose uptake into cells in response to insulin or exercise. Structurally, GLUT4 is a 12-transmembrane protein, with key residues involved in glucose binding and transport located in the membrane-spanning regions. It contains specific motifs that regulate its trafficking and fusion with the plasma membrane, which is essential for its function. Under basal conditions, GLUT4 is sequestered in intracellular vesicles, mainly in the trans-Golgi network (TGN) and endosomes. Upon insulin stimulation or exercise, GLUT4 undergoes translocation to the cell surface, a process facilitated by the PI3K/Akt signaling pathway. This process is tightly regulated by proteins including AS160 and Rab GTPases, which control the mobilization of GLUT4 storage vesicles, and SNAREs (Syntaxin 4, SNAP23, and VAMP2), which mediate vesicle fusion with the plasma membrane. GLUT4 plays a central role in maintaining blood glucose levels by increasing glucose uptake, especially after meals or during physical activity. Its dysfunction is a key factor in the development of insulin resistance and Type 2 diabetes.

Background

GLUT4 (Glucose Transporter Type 4) is a member of the SLC2 family of facilitative glucose transporters, primarily found in insulin-sensitive tissues such as skeletal muscle, adipose tissue, and the heart. It is essential for the regulation of glucose homeostasis, as it facilitates glucose uptake into cells in response to insulin or exercise. Structurally, GLUT4 is a 12-transmembrane protein, with key residues involved in glucose binding and transport located in the membrane-spanning regions. It contains specific motifs that regulate its trafficking and fusion with the plasma membrane, which is essential for its function. Under basal conditions, GLUT4 is sequestered in intracellular vesicles, mainly in the trans-Golgi network (TGN) and endosomes. Upon insulin stimulation or exercise, GLUT4 undergoes translocation to the cell surface, a process facilitated by the PI3K/Akt signaling pathway. This process is tightly regulated by proteins including AS160 and Rab GTPases, which control the mobilization of GLUT4 storage vesicles, and SNAREs (Syntaxin 4, SNAP23, and VAMP2), which mediate vesicle fusion with the plasma membrane. GLUT4 plays a central role in maintaining blood glucose levels by increasing glucose uptake, especially after meals or during physical activity. Its dysfunction is a key factor in the development of insulin resistance and Type 2 diabetes.

References
https://pubmed.ncbi.nlm.nih.gov/11994746/ https://pubmed.ncbi.nlm.nih.gov/17403369/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%