Geminin Rabbit mAb

Catalog No.: F4229

Application: Reactivity: Human, Monkey

Usage Information

Dilution
WB1:1000 IP1:100 IHC1:200 - 1:800 IF1:800 - 1:1600

Application
WB, IP, IHC, IF

Reactivity
Human, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
25 kDa

Datasheet & SDS

Biological Description

Specificity
Geminin Rabbit mAb detects endogenous levels of total geminin protein.

Clone
L11K4

Synonym(s)
Geminin, GMNN

Background
Geminin is a small, unstable regulatory protein expressed in vertebrates and higher eukaryotes that plays dual roles in controlling DNA replication and cell differentiation. Structurally, it contains a coiled-coil domain that mediates dimerization (essential for activity), a bipartite nuclear localization signal adjacent to a destruction box that targets it for ubiquitin-dependent degradation at mitosis, and a Cdt1-binding domain overlapping the dimerization region. Geminin functions by inhibiting the replication licensing factor Cdt1 to prevent DNA re-replication during S and G2 phases, and it also promotes mitotic entry, partly through suppression of the checkpoint kinase Chk1. Beyond cell cycle regulation, geminin influences embryonic development by inducing neural fate, inhibiting eye formation, and altering axial patterning through interactions with transcription factors such as Six3 and Hox proteins, linking its activity to both replication control and cell fate determination.

Background

Geminin is a small, unstable regulatory protein expressed in vertebrates and higher eukaryotes that plays dual roles in controlling DNA replication and cell differentiation. Structurally, it contains a coiled-coil domain that mediates dimerization (essential for activity), a bipartite nuclear localization signal adjacent to a destruction box that targets it for ubiquitin-dependent degradation at mitosis, and a Cdt1-binding domain overlapping the dimerization region. Geminin functions by inhibiting the replication licensing factor Cdt1 to prevent DNA re-replication during S and G2 phases, and it also promotes mitotic entry, partly through suppression of the checkpoint kinase Chk1. Beyond cell cycle regulation, geminin influences embryonic development by inducing neural fate, inhibiting eye formation, and altering axial patterning through interactions with transcription factors such as Six3 and Hox proteins, linking its activity to both replication control and cell fate determination.

References
https://pubmed.ncbi.nlm.nih.gov/15308655/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%