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GBP1 Rabbit mAb

Catalog No.: F3653

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:50 - 1:100 IF1:100 - 1:250

Application
WB, IHC, IF

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
68 kDa 68 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
GBP1 Rabbit mAb detects endogenous levels of total GBP1 protein.

Clone
A8M6

Synonym(s)
Guanylate-binding protein 1, GTP-binding protein 1, Guanine nucleotide-binding protein 1, Interferon-induced guanylate-binding protein 1, GBP-1, HuGBP-1, hGBP1, GBP1

Background
Guanylate‑Binding Protein 1 (GBP1) is a ~65 kDa interferon‑inducible large GTPase of the dynamin superfamily that plays a central role in host innate immunity against intracellular pathogens. While its N‑terminal Large GTPase (LG) domain, with a guanine cap and unique phosphate‑binding sites, provides the rare ability to hydrolyze GTP to both GDP and GMP, the biological significance of GBP1 lies in how these structural features fuel its immune functions. Upon interferon stimulation, GTP binding and hydrolysis drive conformational changes that promote homodimerization and assembly into higher‑order oligomers. A C‑terminal CaaX motif directs GBP1 to membranes, where it forms dense antimicrobial coats around pathogens such as Shigella and Salmonella, restricting their replication. GBP1 also recruits additional immune effectors, promotes pathogen vacuole lysis, and facilitates inflammasome activation, thereby amplifying cytokine responses. In viral infections, it interferes with replication complexes, contributing to antiviral defense. GBP1 influences autophagy by targeting bacterial membranes for degradation and modulates inflammatory signaling pathways, impacting NF‑κB activation and cell survival. Its expression levels and activity correlate with protection in bacterial and viral infections, whereas dysregulation is associated with chronic inflammation and tissue damage.

Background

Guanylate‑Binding Protein 1 (GBP1) is a ~65 kDa interferon‑inducible large GTPase of the dynamin superfamily that plays a central role in host innate immunity against intracellular pathogens. While its N‑terminal Large GTPase (LG) domain, with a guanine cap and unique phosphate‑binding sites, provides the rare ability to hydrolyze GTP to both GDP and GMP, the biological significance of GBP1 lies in how these structural features fuel its immune functions. Upon interferon stimulation, GTP binding and hydrolysis drive conformational changes that promote homodimerization and assembly into higher‑order oligomers. A C‑terminal CaaX motif directs GBP1 to membranes, where it forms dense antimicrobial coats around pathogens such as Shigella and Salmonella, restricting their replication. GBP1 also recruits additional immune effectors, promotes pathogen vacuole lysis, and facilitates inflammasome activation, thereby amplifying cytokine responses. In viral infections, it interferes with replication complexes, contributing to antiviral defense. GBP1 influences autophagy by targeting bacterial membranes for degradation and modulates inflammatory signaling pathways, impacting NF‑κB activation and cell survival. Its expression levels and activity correlate with protection in bacterial and viral infections, whereas dysregulation is associated with chronic inflammation and tissue damage.

References
https://pubmed.ncbi.nlm.nih.gov/39394410/ https://pubmed.ncbi.nlm.nih.gov/32117203/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%