GALT Rabbit mAb

Catalog No.: F3972

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000-1:5000 IP1:10 - 1:100

Application
WB, IP

Reactivity
Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
43 kDa

Datasheet & SDS

Biological Description

Specificity
GALT Rabbit mAb detects endogenous levels of total GALT protein.

Clone
C15E2

Synonym(s)
Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, GALT

Background
GALT (Galactose-1-phosphate uridylyltransferase) is a homodimeric enzyme of the histidine triad (HIT) superfamily, with each of its 379-amino-acid subunits containing an active-site His-Pro-His motif at the dimer interface. It catalyzes a key step in the Leloir pathway, converting galactose-1-phosphate (Gal-1-P) and UDP-glucose (UDP-Glc) into glucose-1-phosphate (Glc-1-P) and UDP-galactose (UDP-Gal) via a ping-pong mechanism. The human GALT gene, located on chromosome 9p13, is expressed in many tissues, particularly the liver, and is especially important in the neonatal period due to the high galactose intake from milk. GALT deficiency, caused by mutations in the GALT gene, leads to type 1 galactosemia—an autosomal recessive metabolic disorder characterized by impaired galactose metabolism—manifesting in varying severity depending on residual enzyme activity.

Background

GALT (Galactose-1-phosphate uridylyltransferase) is a homodimeric enzyme of the histidine triad (HIT) superfamily, with each of its 379-amino-acid subunits containing an active-site His-Pro-His motif at the dimer interface. It catalyzes a key step in the Leloir pathway, converting galactose-1-phosphate (Gal-1-P) and UDP-glucose (UDP-Glc) into glucose-1-phosphate (Glc-1-P) and UDP-galactose (UDP-Gal) via a ping-pong mechanism. The human GALT gene, located on chromosome 9p13, is expressed in many tissues, particularly the liver, and is especially important in the neonatal period due to the high galactose intake from milk. GALT deficiency, caused by mutations in the GALT gene, leads to type 1 galactosemia—an autosomal recessive metabolic disorder characterized by impaired galactose metabolism—manifesting in varying severity depending on residual enzyme activity.

References
https://pubmed.ncbi.nlm.nih.gov/35883524/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%