FoxD3 Rabbit mAb

Catalog No.: F1332

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
60 kDa

Datasheet & SDS

Biological Description

Specificity
FoxD3 Rabbit mAb detects transfected levels of total FoxD3 protein.

Clone
N18E17

Synonym(s)
Forkhead box protein D3, HNF3/FH transcription factor genesis, FOXD3, HFH2

Background
The Foxd3 gene encodes a transcriptional repressor that belongs to the Forkhead Box (Fox) family, a group of proteins defined by a conserved winged-helix DNA-binding domain of approximately 100 amino acids, along with regions that mediate transcriptional activation or repression. Initially identified for its expression in embryonic stem (ES) cells and multipotent neural crest (NC) cells, FoxD3 plays a critical role in early development. As a key transcriptional regulator, FoxD3 is essential for maintaining pluripotency during the pre-implantation and peri-implantation stages of mouse embryogenesis and is also necessary for proper trophoblast development. Beyond its role in early embryonic stages, FoxD3 is crucial for the development and maintenance of the mammalian neural crest. Additionally, FoxD3 participates in a core regulatory network with other key pluripotency factors such as Oct-4 and NANOG, working together to preserve the undifferentiated state of ES cells and regulate cell fate decisions during development.

Background

The Foxd3 gene encodes a transcriptional repressor that belongs to the Forkhead Box (Fox) family, a group of proteins defined by a conserved winged-helix DNA-binding domain of approximately 100 amino acids, along with regions that mediate transcriptional activation or repression. Initially identified for its expression in embryonic stem (ES) cells and multipotent neural crest (NC) cells, FoxD3 plays a critical role in early development. As a key transcriptional regulator, FoxD3 is essential for maintaining pluripotency during the pre-implantation and peri-implantation stages of mouse embryogenesis and is also necessary for proper trophoblast development. Beyond its role in early embryonic stages, FoxD3 is crucial for the development and maintenance of the mammalian neural crest. Additionally, FoxD3 participates in a core regulatory network with other key pluripotency factors such as Oct-4 and NANOG, working together to preserve the undifferentiated state of ES cells and regulate cell fate decisions during development.

References
https://pubmed.ncbi.nlm.nih.gov/12381664/ https://pubmed.ncbi.nlm.nih.gov/18367558/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%