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Fos B Rabbit mAb

Catalog No.: F3379

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:10000 IP1:50 IHC1:1000 IF1:500

Application
WB, IP, IHC, IF

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
36 kDa 38 kDa,48 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse hippocampus; Mouse kidney; Rat hippocampus; Rat kidney; HeLa cell (serum starved overnight, 200nM TPA for 4hr at 10 µg); NIH/3T3 cell (serum starved overnight, serum stimulated for 4hr at 10 µg); C6 cell (serum starved overnight, then serum stimulated for 4hr at 10 µg)
Negative Control	HeLa cell; NIH/3T3 cell; C6 cell

Datasheet & SDS

Biological Description

Specificity
Fos B Rabbit mAb detects endogenous levels of total Fos B protein.

Uniprot ID
P53539

Clone
G14F16

Synonym(s)
G0S3, FOSB, Protein FosB, G0/G1 switch regulatory protein 3, Transcription factor AP-1 subunit FosB

Background
FosB is an immediate early gene and member of the Fos family of transcription factors that regulates gene expression in response to various stimuli, including stress, drugs, and neuronal activity. Structurally, FosB contains a basic leucine zipper (bZIP) domain essential for DNA binding and dimerization with Jun proteins to form active AP-1 transcription factor complexes. The FosB gene consists of four exons and generates multiple splice variants, including full-length FosB, the highly stable ΔFosB, and Δ2ΔFosB, each with distinct regulatory properties. FosB is dynamically expressed across tissues, with prominent induction in the brain, particularly in the nucleus accumbens, following repeated stress or drug exposure. Functionally, ΔFosB accumulates due to the absence of degron domains and phosphorylation at Ser27, enabling long-lasting transcriptional effects that promote reward sensitivity, stress resilience, and responses to antidepressants, thus playing a critical role in neuroplasticity and behavioral adaptation.

Background

FosB is an immediate early gene and member of the Fos family of transcription factors that regulates gene expression in response to various stimuli, including stress, drugs, and neuronal activity. Structurally, FosB contains a basic leucine zipper (bZIP) domain essential for DNA binding and dimerization with Jun proteins to form active AP-1 transcription factor complexes. The FosB gene consists of four exons and generates multiple splice variants, including full-length FosB, the highly stable ΔFosB, and Δ2ΔFosB, each with distinct regulatory properties. FosB is dynamically expressed across tissues, with prominent induction in the brain, particularly in the nucleus accumbens, following repeated stress or drug exposure. Functionally, ΔFosB accumulates due to the absence of degron domains and phosphorylation at Ser27, enabling long-lasting transcriptional effects that promote reward sensitivity, stress resilience, and responses to antidepressants, thus playing a critical role in neuroplasticity and behavioral adaptation.

References
[1] Nestler EJ. Eur J Pharmacol. 2015 Apr 15;753:66-72.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%